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Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents.

Nainys J, Timinskas A, Schneider J, Ulrich RG, Gedvilaite A - PLoS ONE (2015)

Bottom Line: Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus.The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively.In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

View Article: PubMed Central - PubMed

Affiliation: Department of Eukaryote Genetic Engineering, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.

ABSTRACT
Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames for early regulatory proteins large T antigen and small T antigen on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand. Virus-like particles (VLPs) were generated by yeast expression of the VP1 protein of both PyVs. VLP-based ELISA and large T-antigen sequence-targeted polymerase-chain reaction investigations demonstrated signs of infection of these novel PyVs in about 42% of bank voles and 18% of common voles. In most cases only viral DNA, but not VP1-specific antibodies were detected. In additional animals exclusively VP1-specific antibodies, but no viral DNA was detected, indicative for virus clearance. Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus. The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively. In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of 98 representatives of Polyomaviridae family obtained using ML methods for concatenated LTag, STag, VP1 and VP2 protein sequence alignment.The tree is rooted at the most distant PyVs, namely fish polyomaviruses. Numbers at the nodes indicate statistical support (bootstrap values) for branch placements. Red triangles mark taxons with least confidence in their placement, as determined using RogueNaRok. The five lineages: Orthopolyomavirus I, Orthopolyomavirus II, Avipolyomavirus, Malawipolyomavirus and Wukipolyomavirus are highlighted in different colors. Rodent polyomaviruses Murine PyV (MPyV), Hamster PyV (HaPyV), Murine pneumotropic virus (MPtV) and Mastomys PyV (MasPyV) are labeled in green. The novel viruses BVPyV and CVPyV are labeled in blue.
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pone.0140916.g003: Phylogenetic tree of 98 representatives of Polyomaviridae family obtained using ML methods for concatenated LTag, STag, VP1 and VP2 protein sequence alignment.The tree is rooted at the most distant PyVs, namely fish polyomaviruses. Numbers at the nodes indicate statistical support (bootstrap values) for branch placements. Red triangles mark taxons with least confidence in their placement, as determined using RogueNaRok. The five lineages: Orthopolyomavirus I, Orthopolyomavirus II, Avipolyomavirus, Malawipolyomavirus and Wukipolyomavirus are highlighted in different colors. Rodent polyomaviruses Murine PyV (MPyV), Hamster PyV (HaPyV), Murine pneumotropic virus (MPtV) and Mastomys PyV (MasPyV) are labeled in green. The novel viruses BVPyV and CVPyV are labeled in blue.

Mentions: LTag, STag, VP1 and VP2 proteins known to be common for all PyVs were selected for phylogenetic analysis. As the quality of phylogenetic analysis depends highly on the quality of provided sequence alignments, in this study four separate alignments for each LTag, STag, VP1 and VP2 proteins as well as a concatenated one of 98 representative PyVs were produced using three different methods. A maximum likelihood tree with highest bootstrap support values and lowest uncertainty of branch placement was obtained for MAFFT alignment with “—ep 0.123” when the alignment was not reduced by use of GBLOCKS (Fig 3).


Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents.

Nainys J, Timinskas A, Schneider J, Ulrich RG, Gedvilaite A - PLoS ONE (2015)

Phylogenetic tree of 98 representatives of Polyomaviridae family obtained using ML methods for concatenated LTag, STag, VP1 and VP2 protein sequence alignment.The tree is rooted at the most distant PyVs, namely fish polyomaviruses. Numbers at the nodes indicate statistical support (bootstrap values) for branch placements. Red triangles mark taxons with least confidence in their placement, as determined using RogueNaRok. The five lineages: Orthopolyomavirus I, Orthopolyomavirus II, Avipolyomavirus, Malawipolyomavirus and Wukipolyomavirus are highlighted in different colors. Rodent polyomaviruses Murine PyV (MPyV), Hamster PyV (HaPyV), Murine pneumotropic virus (MPtV) and Mastomys PyV (MasPyV) are labeled in green. The novel viruses BVPyV and CVPyV are labeled in blue.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608572&req=5

pone.0140916.g003: Phylogenetic tree of 98 representatives of Polyomaviridae family obtained using ML methods for concatenated LTag, STag, VP1 and VP2 protein sequence alignment.The tree is rooted at the most distant PyVs, namely fish polyomaviruses. Numbers at the nodes indicate statistical support (bootstrap values) for branch placements. Red triangles mark taxons with least confidence in their placement, as determined using RogueNaRok. The five lineages: Orthopolyomavirus I, Orthopolyomavirus II, Avipolyomavirus, Malawipolyomavirus and Wukipolyomavirus are highlighted in different colors. Rodent polyomaviruses Murine PyV (MPyV), Hamster PyV (HaPyV), Murine pneumotropic virus (MPtV) and Mastomys PyV (MasPyV) are labeled in green. The novel viruses BVPyV and CVPyV are labeled in blue.
Mentions: LTag, STag, VP1 and VP2 proteins known to be common for all PyVs were selected for phylogenetic analysis. As the quality of phylogenetic analysis depends highly on the quality of provided sequence alignments, in this study four separate alignments for each LTag, STag, VP1 and VP2 proteins as well as a concatenated one of 98 representative PyVs were produced using three different methods. A maximum likelihood tree with highest bootstrap support values and lowest uncertainty of branch placement was obtained for MAFFT alignment with “—ep 0.123” when the alignment was not reduced by use of GBLOCKS (Fig 3).

Bottom Line: Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus.The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively.In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

View Article: PubMed Central - PubMed

Affiliation: Department of Eukaryote Genetic Engineering, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.

ABSTRACT
Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames for early regulatory proteins large T antigen and small T antigen on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand. Virus-like particles (VLPs) were generated by yeast expression of the VP1 protein of both PyVs. VLP-based ELISA and large T-antigen sequence-targeted polymerase-chain reaction investigations demonstrated signs of infection of these novel PyVs in about 42% of bank voles and 18% of common voles. In most cases only viral DNA, but not VP1-specific antibodies were detected. In additional animals exclusively VP1-specific antibodies, but no viral DNA was detected, indicative for virus clearance. Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus. The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively. In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

No MeSH data available.


Related in: MedlinePlus