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Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents.

Nainys J, Timinskas A, Schneider J, Ulrich RG, Gedvilaite A - PLoS ONE (2015)

Bottom Line: Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus.The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively.In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

View Article: PubMed Central - PubMed

Affiliation: Department of Eukaryote Genetic Engineering, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.

ABSTRACT
Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames for early regulatory proteins large T antigen and small T antigen on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand. Virus-like particles (VLPs) were generated by yeast expression of the VP1 protein of both PyVs. VLP-based ELISA and large T-antigen sequence-targeted polymerase-chain reaction investigations demonstrated signs of infection of these novel PyVs in about 42% of bank voles and 18% of common voles. In most cases only viral DNA, but not VP1-specific antibodies were detected. In additional animals exclusively VP1-specific antibodies, but no viral DNA was detected, indicative for virus clearance. Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus. The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively. In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

No MeSH data available.


Related in: MedlinePlus

Detection of BVPyV (A) and CVPyV (B) VP1 protein VLP formation by electron microscopy.Bars, 100 nm.
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pone.0140916.g002: Detection of BVPyV (A) and CVPyV (B) VP1 protein VLP formation by electron microscopy.Bars, 100 nm.

Mentions: Recombinant VP1 proteins of BVPyV and CVPyV were efficiently generated in yeast. The ability of the VP1proteins to self-assemble into VLPs was tested after purification by electron microscopy (EM) analysis. It was demonstrated that VP1 proteins derived from both new viruses were assembling into PyV-like VLPs (Fig 2A and 2B). The size of BVPyV- derived VLPs was 45–50 nm and that of CVPyV-derived VLPs 50–55 nm in diameter. The VP1 VLPs were used for serological screening of vole CCF samples by indirect ELISA. A parallel analysis of WUPyV-VP1-derived VLPs should allow conclusions on the potential cross-reactivity of antibodies with VLPs of this PyV and the two novel vole PyVs.


Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents.

Nainys J, Timinskas A, Schneider J, Ulrich RG, Gedvilaite A - PLoS ONE (2015)

Detection of BVPyV (A) and CVPyV (B) VP1 protein VLP formation by electron microscopy.Bars, 100 nm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608572&req=5

pone.0140916.g002: Detection of BVPyV (A) and CVPyV (B) VP1 protein VLP formation by electron microscopy.Bars, 100 nm.
Mentions: Recombinant VP1 proteins of BVPyV and CVPyV were efficiently generated in yeast. The ability of the VP1proteins to self-assemble into VLPs was tested after purification by electron microscopy (EM) analysis. It was demonstrated that VP1 proteins derived from both new viruses were assembling into PyV-like VLPs (Fig 2A and 2B). The size of BVPyV- derived VLPs was 45–50 nm and that of CVPyV-derived VLPs 50–55 nm in diameter. The VP1 VLPs were used for serological screening of vole CCF samples by indirect ELISA. A parallel analysis of WUPyV-VP1-derived VLPs should allow conclusions on the potential cross-reactivity of antibodies with VLPs of this PyV and the two novel vole PyVs.

Bottom Line: Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus.The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively.In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

View Article: PubMed Central - PubMed

Affiliation: Department of Eukaryote Genetic Engineering, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.

ABSTRACT
Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames for early regulatory proteins large T antigen and small T antigen on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand. Virus-like particles (VLPs) were generated by yeast expression of the VP1 protein of both PyVs. VLP-based ELISA and large T-antigen sequence-targeted polymerase-chain reaction investigations demonstrated signs of infection of these novel PyVs in about 42% of bank voles and 18% of common voles. In most cases only viral DNA, but not VP1-specific antibodies were detected. In additional animals exclusively VP1-specific antibodies, but no viral DNA was detected, indicative for virus clearance. Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus. The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively. In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

No MeSH data available.


Related in: MedlinePlus