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Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents.

Nainys J, Timinskas A, Schneider J, Ulrich RG, Gedvilaite A - PLoS ONE (2015)

Bottom Line: Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus.The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively.In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

View Article: PubMed Central - PubMed

Affiliation: Department of Eukaryote Genetic Engineering, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.

ABSTRACT
Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames for early regulatory proteins large T antigen and small T antigen on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand. Virus-like particles (VLPs) were generated by yeast expression of the VP1 protein of both PyVs. VLP-based ELISA and large T-antigen sequence-targeted polymerase-chain reaction investigations demonstrated signs of infection of these novel PyVs in about 42% of bank voles and 18% of common voles. In most cases only viral DNA, but not VP1-specific antibodies were detected. In additional animals exclusively VP1-specific antibodies, but no viral DNA was detected, indicative for virus clearance. Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus. The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively. In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

No MeSH data available.


Related in: MedlinePlus

Schematic presentation of the genome organization of BVPyV (A) and CVPyV (B) and alignment of ORI region sequences of BVPyV strains (KS/14/281 and KS/13/999) and CVPyV strain KS/13/947 (C).The positions of LTag introns are shown uncolored. Putative LTag binding sites (GAGGC pentanucleotides) are shown in red; AT-rich region/TATA box is shown in green; palindromic repeats shown in magenta; early palindrome (EP) region is underlined; LTag ATG codon is shown in blue (according [38, 39]).
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pone.0140916.g001: Schematic presentation of the genome organization of BVPyV (A) and CVPyV (B) and alignment of ORI region sequences of BVPyV strains (KS/14/281 and KS/13/999) and CVPyV strain KS/13/947 (C).The positions of LTag introns are shown uncolored. Putative LTag binding sites (GAGGC pentanucleotides) are shown in red; AT-rich region/TATA box is shown in green; palindromic repeats shown in magenta; early palindrome (EP) region is underlined; LTag ATG codon is shown in blue (according [38, 39]).

Mentions: All seven cloned and sequenced genomes had the typical PyV genome organization, including putative ORFs for early regulatory proteins (LTag and STag) on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand (Fig 1A and 1B). Both regions were separated by the NCCR. LTag and STag encoding sequences were identified based on homology to known PyV proteins, on genomic location and sequences resembling classical eukaryotic splice donor and acceptor consensus signals. Two splicing sites were detected for LTag early transcript (S2 Table). Such analysis showed that LTag mRNA contains three exons encoding 651 aa protein for four BVPyV strains (or 650 amino acids, aa, for KS/13/999) and 642 aa for both CVPyV strains. Further, the early genome regions of CVPyV and BVPyV do not encode MTag or ALTO proteins, which is in contrast to HaPyV or MPyV [7]. However, further experiments with mRNA isolated from virus infected cells are needed to fully investigate the presence of LTag splicing variants because LTag in other rodent PyVs are encoded by only two exons. An additional putative ORF (tentatively named X-ORF) was found in the NCCR region of BVPyV and CVPyV (Fig 1A and 1B, magenta arrow). ORF sizes and locations were not conserved between BVPyV strains KS/14/281 (162 bp) and KS/13/999 (189 bp) or CVPyV (426 bp) genomes (Fig 1 and S2 Table). The putative proteins encoded by these X-ORFs in BVPyV strains KS/14/281 (53 aa) and KS/13/999 (62 aa) or both CVPyV strains (141 aa) had different length, were less than 35% identical in their amino acid sequences and had no sequence similarity to any protein from protein data bases, so they are unlikely to be synthesized and/or of functional relevance.


Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents.

Nainys J, Timinskas A, Schneider J, Ulrich RG, Gedvilaite A - PLoS ONE (2015)

Schematic presentation of the genome organization of BVPyV (A) and CVPyV (B) and alignment of ORI region sequences of BVPyV strains (KS/14/281 and KS/13/999) and CVPyV strain KS/13/947 (C).The positions of LTag introns are shown uncolored. Putative LTag binding sites (GAGGC pentanucleotides) are shown in red; AT-rich region/TATA box is shown in green; palindromic repeats shown in magenta; early palindrome (EP) region is underlined; LTag ATG codon is shown in blue (according [38, 39]).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608572&req=5

pone.0140916.g001: Schematic presentation of the genome organization of BVPyV (A) and CVPyV (B) and alignment of ORI region sequences of BVPyV strains (KS/14/281 and KS/13/999) and CVPyV strain KS/13/947 (C).The positions of LTag introns are shown uncolored. Putative LTag binding sites (GAGGC pentanucleotides) are shown in red; AT-rich region/TATA box is shown in green; palindromic repeats shown in magenta; early palindrome (EP) region is underlined; LTag ATG codon is shown in blue (according [38, 39]).
Mentions: All seven cloned and sequenced genomes had the typical PyV genome organization, including putative ORFs for early regulatory proteins (LTag and STag) on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand (Fig 1A and 1B). Both regions were separated by the NCCR. LTag and STag encoding sequences were identified based on homology to known PyV proteins, on genomic location and sequences resembling classical eukaryotic splice donor and acceptor consensus signals. Two splicing sites were detected for LTag early transcript (S2 Table). Such analysis showed that LTag mRNA contains three exons encoding 651 aa protein for four BVPyV strains (or 650 amino acids, aa, for KS/13/999) and 642 aa for both CVPyV strains. Further, the early genome regions of CVPyV and BVPyV do not encode MTag or ALTO proteins, which is in contrast to HaPyV or MPyV [7]. However, further experiments with mRNA isolated from virus infected cells are needed to fully investigate the presence of LTag splicing variants because LTag in other rodent PyVs are encoded by only two exons. An additional putative ORF (tentatively named X-ORF) was found in the NCCR region of BVPyV and CVPyV (Fig 1A and 1B, magenta arrow). ORF sizes and locations were not conserved between BVPyV strains KS/14/281 (162 bp) and KS/13/999 (189 bp) or CVPyV (426 bp) genomes (Fig 1 and S2 Table). The putative proteins encoded by these X-ORFs in BVPyV strains KS/14/281 (53 aa) and KS/13/999 (62 aa) or both CVPyV strains (141 aa) had different length, were less than 35% identical in their amino acid sequences and had no sequence similarity to any protein from protein data bases, so they are unlikely to be synthesized and/or of functional relevance.

Bottom Line: Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus.The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively.In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

View Article: PubMed Central - PubMed

Affiliation: Department of Eukaryote Genetic Engineering, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania.

ABSTRACT
Two novel polyomaviruses (PyVs) were identified in kidney and chest-cavity fluid samples of wild bank voles (Myodes glareolus) and common voles (Microtus arvalis) collected in Germany. All cloned and sequenced genomes had the typical PyV genome organization, including putative open reading frames for early regulatory proteins large T antigen and small T antigen on one strand and for structural late proteins (VP1, VP2 and VP3) on the other strand. Virus-like particles (VLPs) were generated by yeast expression of the VP1 protein of both PyVs. VLP-based ELISA and large T-antigen sequence-targeted polymerase-chain reaction investigations demonstrated signs of infection of these novel PyVs in about 42% of bank voles and 18% of common voles. In most cases only viral DNA, but not VP1-specific antibodies were detected. In additional animals exclusively VP1-specific antibodies, but no viral DNA was detected, indicative for virus clearance. Phylogenetic and clustering analysis including all known PyV genomes placed novel bank vole and common vole PyVs amongst members of the tentative Wukipolymavirus genus. The other known four rodent PyVs, Murine PyV and Hamster PyV, and Murine pneumotropic virus and Mastomys PyV belong to different phylogenetic clades, tentatively named Orthopolyomavirus I and Orthopolyomavirus II, respectively. In conclusion, the finding of novel vole-borne PyVs may suggest an evolutionary origin of ancient wukipolyomaviruses in rodents and may offer the possibility to develop a vole-based animal model for human wukipolyomaviruses.

No MeSH data available.


Related in: MedlinePlus