Limits...
Comparison of the Cobas 4800 HPV and HPV 9G DNA Chip Tests for Detection of High-Risk Human Papillomavirus in Cervical Specimens of Women with Consecutive Positive HPV Tests But Negative Pap Smears.

Jun SY, Park ES, Kim J, Kang J, Lee JJ, Bae Y, Kim SI, Maeng LS - PLoS ONE (2015)

Bottom Line: Detecting high-risk (HR) HPV is important for clinical management of women with persistent HPV-positive and Pap-negative results.For HR genotypes other than HPV types 16 and 18, the two tests agreed for 81.1% of the samples.The sensitivity of both assays for detecting HR HPV was 100%, regardless of HR genotypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Incheon St. Mary's Hospital, Incheon, The Catholic University of Korea, Incheon, Republic of Korea.

ABSTRACT
Detecting high-risk (HR) HPV is important for clinical management of women with persistent HPV-positive and Pap-negative results. The Cobas 4800 HPV test is the first FDA-approved HPV DNA test that can be used alone as a first-line screening tool. The HPV 9G DNA chip test is a PCR-based DNA microarray assay. We evaluated the patients of consecutive HPV-positivity on HPV 9G DNA chip test without cytologic abnormalities. We then compared the performances of HPV 9G DNA chip and the Cobas 4800 HPV tests for detecting HR HPV with each other and confirmed HPV genotyping using direct sequencing. All 214 liquid-based cytology specimens were collected from 100 women with consecutive HPV-positive and Pap-negative results on the HPV 9G DNA chip test between May 2012 and Dec 2013, but only 180 specimens were available for comparing HPV test results. The HPV 9G DNA chip and the Cobas 4800 HPV tests agreed with each other in 81.7% of the samples, and the concordance rate was greater than 97.2% for detecting HPV-16 or -18. For HR genotypes other than HPV types 16 and 18, the two tests agreed for 81.1% of the samples. The sensitivity of both assays for detecting HR HPV was 100%, regardless of HR genotypes. The HPV 9G DNA chip test may be as effective as the Cobas 4800 HPV test in detecting HR HPV, and has a similar ability to identify HPV-16 and -18.

No MeSH data available.


Related in: MedlinePlus

Concordance between the results of two HPV DNA tests according to high-risk (HR) HPV genotypes.(Abbreviations: k, kappa coefficient).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4607436&req=5

pone.0140336.g001: Concordance between the results of two HPV DNA tests according to high-risk (HR) HPV genotypes.(Abbreviations: k, kappa coefficient).

Mentions: Regardless of HR HPV genotypes, the results of the two tests moderately agreed in 81.7% of all cases (k = 0.598) when detecting HR HPV (Fig 1). The proportions of positive and negative agreement for HPV status of any of the 14 HR HPV types were 0.736 and 0.860, respectively. The proportion of positive agreement was lower than that of negative one, ranging from 0.660 in HR HPV-other than types 16 and 18 to 0.857 in HPV-18. Among the 23 samples with positive HR HPV results only on the Cobas 4800 HPV test, 11 were negative for HR genotypes by sequencing. On the other hand, 3 of 10 specimens which were positive for HR HPV only on the HPV 9G DNA chip test were negative for HR genotypes by sequencing. The other specimens with discrepant results were not able to be sequenced due to a lack of remnant DNA.


Comparison of the Cobas 4800 HPV and HPV 9G DNA Chip Tests for Detection of High-Risk Human Papillomavirus in Cervical Specimens of Women with Consecutive Positive HPV Tests But Negative Pap Smears.

Jun SY, Park ES, Kim J, Kang J, Lee JJ, Bae Y, Kim SI, Maeng LS - PLoS ONE (2015)

Concordance between the results of two HPV DNA tests according to high-risk (HR) HPV genotypes.(Abbreviations: k, kappa coefficient).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4607436&req=5

pone.0140336.g001: Concordance between the results of two HPV DNA tests according to high-risk (HR) HPV genotypes.(Abbreviations: k, kappa coefficient).
Mentions: Regardless of HR HPV genotypes, the results of the two tests moderately agreed in 81.7% of all cases (k = 0.598) when detecting HR HPV (Fig 1). The proportions of positive and negative agreement for HPV status of any of the 14 HR HPV types were 0.736 and 0.860, respectively. The proportion of positive agreement was lower than that of negative one, ranging from 0.660 in HR HPV-other than types 16 and 18 to 0.857 in HPV-18. Among the 23 samples with positive HR HPV results only on the Cobas 4800 HPV test, 11 were negative for HR genotypes by sequencing. On the other hand, 3 of 10 specimens which were positive for HR HPV only on the HPV 9G DNA chip test were negative for HR genotypes by sequencing. The other specimens with discrepant results were not able to be sequenced due to a lack of remnant DNA.

Bottom Line: Detecting high-risk (HR) HPV is important for clinical management of women with persistent HPV-positive and Pap-negative results.For HR genotypes other than HPV types 16 and 18, the two tests agreed for 81.1% of the samples.The sensitivity of both assays for detecting HR HPV was 100%, regardless of HR genotypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Incheon St. Mary's Hospital, Incheon, The Catholic University of Korea, Incheon, Republic of Korea.

ABSTRACT
Detecting high-risk (HR) HPV is important for clinical management of women with persistent HPV-positive and Pap-negative results. The Cobas 4800 HPV test is the first FDA-approved HPV DNA test that can be used alone as a first-line screening tool. The HPV 9G DNA chip test is a PCR-based DNA microarray assay. We evaluated the patients of consecutive HPV-positivity on HPV 9G DNA chip test without cytologic abnormalities. We then compared the performances of HPV 9G DNA chip and the Cobas 4800 HPV tests for detecting HR HPV with each other and confirmed HPV genotyping using direct sequencing. All 214 liquid-based cytology specimens were collected from 100 women with consecutive HPV-positive and Pap-negative results on the HPV 9G DNA chip test between May 2012 and Dec 2013, but only 180 specimens were available for comparing HPV test results. The HPV 9G DNA chip and the Cobas 4800 HPV tests agreed with each other in 81.7% of the samples, and the concordance rate was greater than 97.2% for detecting HPV-16 or -18. For HR genotypes other than HPV types 16 and 18, the two tests agreed for 81.1% of the samples. The sensitivity of both assays for detecting HR HPV was 100%, regardless of HR genotypes. The HPV 9G DNA chip test may be as effective as the Cobas 4800 HPV test in detecting HR HPV, and has a similar ability to identify HPV-16 and -18.

No MeSH data available.


Related in: MedlinePlus