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W3 Is a New Wax Locus That Is Essential for Biosynthesis of β-Diketone, Development of Glaucousness, and Reduction of Cuticle Permeability in Common Wheat.

Zhang Z, Wei W, Zhu H, Challa GS, Bi C, Trick HN, Li W - PLoS ONE (2015)

Bottom Line: W3 is essential for β-diketone biosynthesis but suppresses its hydroxylation.Loss-of-function mutation w3 significantly increased cuticle permeability in terms of water loss and chlorophyll efflux.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, 57007, United States of America.

ABSTRACT

Key message: W3 is essential for β-diketone biosynthesis but suppresses its hydroxylation. Loss-of-function mutation w3 significantly increased cuticle permeability in terms of water loss and chlorophyll efflux.

No MeSH data available.


SEM micrographs of cuticle surfaces of flag leaf sheaths.(A) BW and (B) w3 mutant. The scale bars indicated 1 μm.
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pone.0140524.g004: SEM micrographs of cuticle surfaces of flag leaf sheaths.(A) BW and (B) w3 mutant. The scale bars indicated 1 μm.

Mentions: At stage F10.5.1, wheat plants are flowering, and glaucousness is fully developed. We inspected the cuticle surfaces of flag leaf sheaths of BW and w3 mutant under a scanning electron microscope (SEM) at this developmental stage. The electron micrographs showed clear differences between BW and w3 mutant (Fig 4). In BW, cuticle surface was covered with thick meshwork of long wax crystal tubes (Fig 4A). In contrast, short wax bodies were appressed to the leaf cuticle surface of w3 mutant at low density (Fig 4B). This result indicated that the W3 locus has a major effect on cuticle, epicuticular wax in particular, development. Considering that the great contrast in wax crystal morphology and density between BW and w3 mutant was found in the flag leaf sheath, we used this tissue for measuring cuticle permeability, profiling composition of the cuticular waxes and quantifying transcription of cuticle genes.


W3 Is a New Wax Locus That Is Essential for Biosynthesis of β-Diketone, Development of Glaucousness, and Reduction of Cuticle Permeability in Common Wheat.

Zhang Z, Wei W, Zhu H, Challa GS, Bi C, Trick HN, Li W - PLoS ONE (2015)

SEM micrographs of cuticle surfaces of flag leaf sheaths.(A) BW and (B) w3 mutant. The scale bars indicated 1 μm.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4607432&req=5

pone.0140524.g004: SEM micrographs of cuticle surfaces of flag leaf sheaths.(A) BW and (B) w3 mutant. The scale bars indicated 1 μm.
Mentions: At stage F10.5.1, wheat plants are flowering, and glaucousness is fully developed. We inspected the cuticle surfaces of flag leaf sheaths of BW and w3 mutant under a scanning electron microscope (SEM) at this developmental stage. The electron micrographs showed clear differences between BW and w3 mutant (Fig 4). In BW, cuticle surface was covered with thick meshwork of long wax crystal tubes (Fig 4A). In contrast, short wax bodies were appressed to the leaf cuticle surface of w3 mutant at low density (Fig 4B). This result indicated that the W3 locus has a major effect on cuticle, epicuticular wax in particular, development. Considering that the great contrast in wax crystal morphology and density between BW and w3 mutant was found in the flag leaf sheath, we used this tissue for measuring cuticle permeability, profiling composition of the cuticular waxes and quantifying transcription of cuticle genes.

Bottom Line: W3 is essential for β-diketone biosynthesis but suppresses its hydroxylation.Loss-of-function mutation w3 significantly increased cuticle permeability in terms of water loss and chlorophyll efflux.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, 57007, United States of America.

ABSTRACT

Key message: W3 is essential for β-diketone biosynthesis but suppresses its hydroxylation. Loss-of-function mutation w3 significantly increased cuticle permeability in terms of water loss and chlorophyll efflux.

No MeSH data available.