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Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco.

Xiang Y, Lu YH, Song M, Wang Y, Xu W, Wu L, Wang H, Ma Z - PLoS ONE (2015)

Bottom Line: Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants.Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions.Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants.

View Article: PubMed Central - PubMed

Affiliation: Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang, China; Crop Genomics and Bioinformatics Center and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants. However, the roles played by this protein in salt stress responses in wheat (Triticum aestivum) remain obscure. In this study, three TaCRT genes were identified in wheat and named TaCRT1, TaCRT2 and TaCRT3-1 based on their sequence characteristics and their high homology to other known CRT genes. Quantitative real-time PCR expression data revealed that these three genes exhibit different expression patterns in different tissues and are strongly induced under salt stress in wheat. The calcium-binding properties of the purified recombinant TaCRT1 protein were determined using a PIPES/Arsenazo III analysis. TaCRT1 gene overexpression in Nicotiana tabacum decreased salt stress damage in transgenic tobacco plants. Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions. Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants.

No MeSH data available.


Related in: MedlinePlus

TaCRT1 segments in transgenic tobacco exhibited greater chlorophyll content.(A) Phenotypes of transgenic tobacco lines under irrigation with 250 mM NaCl every 2 d. (B) Response of leaf discs obtained from WT and transgenic tobacco lines floating on a 400 mM NaCl solution. (C) Chlorophyll content of leaf discs obtained from WT and transgenic tobacco lines. WT represents non-transgenic tobacco; T6, T19 and T27 represent transgenic tobacco lines. D0 represents no treatment, D20 represents NaCl treatment for 20 d; Ca: chlorophyll a; Cb: chlorophyll b. Error bars represent the standard deviation of results obtained for three replicate experiments; asterisks indicate significant differences from WT plants at P = 0.05.
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pone.0140591.g006: TaCRT1 segments in transgenic tobacco exhibited greater chlorophyll content.(A) Phenotypes of transgenic tobacco lines under irrigation with 250 mM NaCl every 2 d. (B) Response of leaf discs obtained from WT and transgenic tobacco lines floating on a 400 mM NaCl solution. (C) Chlorophyll content of leaf discs obtained from WT and transgenic tobacco lines. WT represents non-transgenic tobacco; T6, T19 and T27 represent transgenic tobacco lines. D0 represents no treatment, D20 represents NaCl treatment for 20 d; Ca: chlorophyll a; Cb: chlorophyll b. Error bars represent the standard deviation of results obtained for three replicate experiments; asterisks indicate significant differences from WT plants at P = 0.05.

Mentions: The growth of T3 transgenic tobacco seedlings of T6 (entire TaCRT1), T19 (omission of N-domain), T27 (omission of N- and P-domain) and WT seedlings was examined under exposure to 250 mM NaCl stress (Fig 6A). In each case, the growth of the transgenic seedlings was indistinguishable from that of WT seedlings grown under normal conditions. The results showed that the transgenic lines overexpressing the entire TaCRT1 gene or partial TaCRT1 segments exhibited better tolerance to salinity stress than their WT counterparts.


Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco.

Xiang Y, Lu YH, Song M, Wang Y, Xu W, Wu L, Wang H, Ma Z - PLoS ONE (2015)

TaCRT1 segments in transgenic tobacco exhibited greater chlorophyll content.(A) Phenotypes of transgenic tobacco lines under irrigation with 250 mM NaCl every 2 d. (B) Response of leaf discs obtained from WT and transgenic tobacco lines floating on a 400 mM NaCl solution. (C) Chlorophyll content of leaf discs obtained from WT and transgenic tobacco lines. WT represents non-transgenic tobacco; T6, T19 and T27 represent transgenic tobacco lines. D0 represents no treatment, D20 represents NaCl treatment for 20 d; Ca: chlorophyll a; Cb: chlorophyll b. Error bars represent the standard deviation of results obtained for three replicate experiments; asterisks indicate significant differences from WT plants at P = 0.05.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4607401&req=5

pone.0140591.g006: TaCRT1 segments in transgenic tobacco exhibited greater chlorophyll content.(A) Phenotypes of transgenic tobacco lines under irrigation with 250 mM NaCl every 2 d. (B) Response of leaf discs obtained from WT and transgenic tobacco lines floating on a 400 mM NaCl solution. (C) Chlorophyll content of leaf discs obtained from WT and transgenic tobacco lines. WT represents non-transgenic tobacco; T6, T19 and T27 represent transgenic tobacco lines. D0 represents no treatment, D20 represents NaCl treatment for 20 d; Ca: chlorophyll a; Cb: chlorophyll b. Error bars represent the standard deviation of results obtained for three replicate experiments; asterisks indicate significant differences from WT plants at P = 0.05.
Mentions: The growth of T3 transgenic tobacco seedlings of T6 (entire TaCRT1), T19 (omission of N-domain), T27 (omission of N- and P-domain) and WT seedlings was examined under exposure to 250 mM NaCl stress (Fig 6A). In each case, the growth of the transgenic seedlings was indistinguishable from that of WT seedlings grown under normal conditions. The results showed that the transgenic lines overexpressing the entire TaCRT1 gene or partial TaCRT1 segments exhibited better tolerance to salinity stress than their WT counterparts.

Bottom Line: Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants.Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions.Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants.

View Article: PubMed Central - PubMed

Affiliation: Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang, China; Crop Genomics and Bioinformatics Center and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants. However, the roles played by this protein in salt stress responses in wheat (Triticum aestivum) remain obscure. In this study, three TaCRT genes were identified in wheat and named TaCRT1, TaCRT2 and TaCRT3-1 based on their sequence characteristics and their high homology to other known CRT genes. Quantitative real-time PCR expression data revealed that these three genes exhibit different expression patterns in different tissues and are strongly induced under salt stress in wheat. The calcium-binding properties of the purified recombinant TaCRT1 protein were determined using a PIPES/Arsenazo III analysis. TaCRT1 gene overexpression in Nicotiana tabacum decreased salt stress damage in transgenic tobacco plants. Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions. Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants.

No MeSH data available.


Related in: MedlinePlus