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MicroRNA profiles reveal female allotetraploid hybrid fertility.

Zhou R, Wu Y, Tao M, Zhang C, Liu S - BMC Genet. (2015)

Bottom Line: The differential expressions of miRNAs were confirmed by RT-PCR.The results indicated that most of the highly expressed miRNAs were closely correlated with ovary maturation, and displayed no significant differences in expression.Moreover, 34 up-regulated and nine down-regulated miRNAs were found in 4nAT.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, People's Republic of China. zhourong_1218@163.com.

ABSTRACT

Background: The bisexual fertile tetraploid fish is important in biological evolution. Tetraploid fish fertility is the key factor for stable inheritance. Therefore, elucidating tetraploid fish fertility at the molecular level is essential. MicroRNAs regulate gene expression and are involved in many aspects of gonad development.

Methods: Total RNA was isolated using TRIzol, followed by constructing small RNA libraries. And then, the qualified libraries were sequenced with the HiSeq 2500 SE50 system. The obtained clean reads were analyzed to identify conserved and novel miRNAs, and evaluate the expression, and also predict the target genes. The differential expressions of miRNAs were confirmed by RT-PCR.

Results: In this study, allotetraploid hybrid fish (4nAT) and diploid red crucian carp (RCC) ovaries were used to compare miRNA profiles. The results indicated that most of the highly expressed miRNAs were closely correlated with ovary maturation, and displayed no significant differences in expression. Moreover, 34 up-regulated and nine down-regulated miRNAs were found in 4nAT. The differentially expressed miRNAs were primarily involved in metabolism, defense mechanisms, and cytoskeleton production.

Conclusions: This is the first study to provide new epigenetic evidences for tetraploid fish fertility and phenotypic changes as a result of increased ploidy.

No MeSH data available.


Related in: MedlinePlus

Base bias of small RNA at each position in diploid RCC (a) and 4nAT (b)
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Fig2: Base bias of small RNA at each position in diploid RCC (a) and 4nAT (b)

Mentions: The length distribution of clean reads mainly concentrated in two regions, 21–23 nt and 26–29 nt (Fig. 1a). However, the 21–23 nt reads were most likely to be the miRNAs (Fig. 1b). Statistical analysis of the base sequences indicated that the first base is biased to “U”, the tenth base biased to “A”, and the probability of “U” at the second to forth bases was very low (Fig. 2).Fig. 1


MicroRNA profiles reveal female allotetraploid hybrid fertility.

Zhou R, Wu Y, Tao M, Zhang C, Liu S - BMC Genet. (2015)

Base bias of small RNA at each position in diploid RCC (a) and 4nAT (b)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4607245&req=5

Fig2: Base bias of small RNA at each position in diploid RCC (a) and 4nAT (b)
Mentions: The length distribution of clean reads mainly concentrated in two regions, 21–23 nt and 26–29 nt (Fig. 1a). However, the 21–23 nt reads were most likely to be the miRNAs (Fig. 1b). Statistical analysis of the base sequences indicated that the first base is biased to “U”, the tenth base biased to “A”, and the probability of “U” at the second to forth bases was very low (Fig. 2).Fig. 1

Bottom Line: The differential expressions of miRNAs were confirmed by RT-PCR.The results indicated that most of the highly expressed miRNAs were closely correlated with ovary maturation, and displayed no significant differences in expression.Moreover, 34 up-regulated and nine down-regulated miRNAs were found in 4nAT.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, People's Republic of China. zhourong_1218@163.com.

ABSTRACT

Background: The bisexual fertile tetraploid fish is important in biological evolution. Tetraploid fish fertility is the key factor for stable inheritance. Therefore, elucidating tetraploid fish fertility at the molecular level is essential. MicroRNAs regulate gene expression and are involved in many aspects of gonad development.

Methods: Total RNA was isolated using TRIzol, followed by constructing small RNA libraries. And then, the qualified libraries were sequenced with the HiSeq 2500 SE50 system. The obtained clean reads were analyzed to identify conserved and novel miRNAs, and evaluate the expression, and also predict the target genes. The differential expressions of miRNAs were confirmed by RT-PCR.

Results: In this study, allotetraploid hybrid fish (4nAT) and diploid red crucian carp (RCC) ovaries were used to compare miRNA profiles. The results indicated that most of the highly expressed miRNAs were closely correlated with ovary maturation, and displayed no significant differences in expression. Moreover, 34 up-regulated and nine down-regulated miRNAs were found in 4nAT. The differentially expressed miRNAs were primarily involved in metabolism, defense mechanisms, and cytoskeleton production.

Conclusions: This is the first study to provide new epigenetic evidences for tetraploid fish fertility and phenotypic changes as a result of increased ploidy.

No MeSH data available.


Related in: MedlinePlus