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Intracellular calcium levels as screening tool for nanoparticle toxicity.

Meindl C, Kueznik T, Bösch M, Roblegg E, Fröhlich E - J Appl Toxicol (2015)

Bottom Line: Effects occurring upon short contact of particles with cells, for instance in the systemic blood circulation, could be identified according to increases of intracellular [Ca(2+) ] levels, which are caused by variety of toxic stimuli.Negatively charged, neutral and positively charged polystyrene particles of different sizes were used to study the role of size and surface properties on viability, membrane disruption, apoptosis, lysosome function, intracellular [Ca(2+) ] levels and generation of oxidative stress.Silica particles served to test this hypothesis.

View Article: PubMed Central - PubMed

Affiliation: Center for Medical Research, Medical University of Graz, Austria.

No MeSH data available.


Related in: MedlinePlus

EAhy926 cells exposed to PS particles for 24 h and assessed for viability (MTS, a) and membrane integrity (LDH release, b). Testing for apoptosis by caspase 3/7 activation (c) was performed after 8 h. The most cytotoxic particles PPS20 and AMI20 particles are characterized by decrease in viability and increase of LDH release and caspase 3/7 activation. Means ± SD of four independent experiments are shown. LDH, lactic dehydrogenase.
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Figure 2: EAhy926 cells exposed to PS particles for 24 h and assessed for viability (MTS, a) and membrane integrity (LDH release, b). Testing for apoptosis by caspase 3/7 activation (c) was performed after 8 h. The most cytotoxic particles PPS20 and AMI20 particles are characterized by decrease in viability and increase of LDH release and caspase 3/7 activation. Means ± SD of four independent experiments are shown. LDH, lactic dehydrogenase.

Mentions: Cytotoxicity screening with EAhy926 cells was performed to identify toxic ranges of NPs and in SH-SY5Y cells used for the measurements of intracellular [Ca2+] levels. Small particles of all charges acted more cytotoxic than 200 nm particles (Fig. 2a, Table 2). Among the small particles, PPS20 and AMI20 were more cytotoxic than CPS20 and APS20 particles. There was no significant decrease in viability after exposure up to 200 μgml−1 PS particles of 200 nm of size (Fig. 1S, Supplementary Material).


Intracellular calcium levels as screening tool for nanoparticle toxicity.

Meindl C, Kueznik T, Bösch M, Roblegg E, Fröhlich E - J Appl Toxicol (2015)

EAhy926 cells exposed to PS particles for 24 h and assessed for viability (MTS, a) and membrane integrity (LDH release, b). Testing for apoptosis by caspase 3/7 activation (c) was performed after 8 h. The most cytotoxic particles PPS20 and AMI20 particles are characterized by decrease in viability and increase of LDH release and caspase 3/7 activation. Means ± SD of four independent experiments are shown. LDH, lactic dehydrogenase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4606983&req=5

Figure 2: EAhy926 cells exposed to PS particles for 24 h and assessed for viability (MTS, a) and membrane integrity (LDH release, b). Testing for apoptosis by caspase 3/7 activation (c) was performed after 8 h. The most cytotoxic particles PPS20 and AMI20 particles are characterized by decrease in viability and increase of LDH release and caspase 3/7 activation. Means ± SD of four independent experiments are shown. LDH, lactic dehydrogenase.
Mentions: Cytotoxicity screening with EAhy926 cells was performed to identify toxic ranges of NPs and in SH-SY5Y cells used for the measurements of intracellular [Ca2+] levels. Small particles of all charges acted more cytotoxic than 200 nm particles (Fig. 2a, Table 2). Among the small particles, PPS20 and AMI20 were more cytotoxic than CPS20 and APS20 particles. There was no significant decrease in viability after exposure up to 200 μgml−1 PS particles of 200 nm of size (Fig. 1S, Supplementary Material).

Bottom Line: Effects occurring upon short contact of particles with cells, for instance in the systemic blood circulation, could be identified according to increases of intracellular [Ca(2+) ] levels, which are caused by variety of toxic stimuli.Negatively charged, neutral and positively charged polystyrene particles of different sizes were used to study the role of size and surface properties on viability, membrane disruption, apoptosis, lysosome function, intracellular [Ca(2+) ] levels and generation of oxidative stress.Silica particles served to test this hypothesis.

View Article: PubMed Central - PubMed

Affiliation: Center for Medical Research, Medical University of Graz, Austria.

No MeSH data available.


Related in: MedlinePlus