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Loss of lysyl oxidase-like 3 causes cleft palate and spinal deformity in mice.

Zhang J, Yang R, Liu Z, Hou C, Zong W, Zhang A, Sun X, Gao J - Hum. Mol. Genet. (2015)

Bottom Line: Failure of any one of these processes can result in embryonic malformation.We found that the obvious decrease of collagen cross-links in palate and spine that was induced by the lack of LOXL3 resulted in cleft palate and spinal deformity.The Loxl3 gene may be a candidate disease gene resulting in cleft palate and spinal deformity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, 27 Shanda Nanlu, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus

Hydroxyproline and desmosine changes. Eviscerated embryos with craniofacial bones and spines were detected at E14.5. (A) The hydroxyproline assay demonstrated that the hydroxyproline content of Loxl3−/− mice compared with Loxl3+/+ mice was significantly decreased (n = 5 in each group, mean ± SD, **P < 0.01). For Loxl3+/− mice, the hydroxyproline content was also less than that of Loxl3+/+ mice (n = 5 in each group, mean ± SD,*P < 0.05). (B) The desmosine concentration was analysed by ELISA. No significant difference was found among the three groups (n = 5 in each group).
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DDV333F11: Hydroxyproline and desmosine changes. Eviscerated embryos with craniofacial bones and spines were detected at E14.5. (A) The hydroxyproline assay demonstrated that the hydroxyproline content of Loxl3−/− mice compared with Loxl3+/+ mice was significantly decreased (n = 5 in each group, mean ± SD, **P < 0.01). For Loxl3+/− mice, the hydroxyproline content was also less than that of Loxl3+/+ mice (n = 5 in each group, mean ± SD,*P < 0.05). (B) The desmosine concentration was analysed by ELISA. No significant difference was found among the three groups (n = 5 in each group).

Mentions: To examine the amount of collagen fibres, a hydroxyproline assay was used to quantify the collagen content in eviscerated embryos at E14.5. The hydroxyproline content of Loxl3−/− mice was significantly less than that of Loxl3+/+ mice (P < 0.01, Fig. 11A). A decrease of the hydroxyproline content in Loxl3+/− mice was also observed (P < 0.05, Fig. 11A). We also examined the concentration of desmosine, a cross-linking amino acid of mature elastin. There was no significant difference in desmosine concentrations among the three groups (Fig. 11B).Figure 11.


Loss of lysyl oxidase-like 3 causes cleft palate and spinal deformity in mice.

Zhang J, Yang R, Liu Z, Hou C, Zong W, Zhang A, Sun X, Gao J - Hum. Mol. Genet. (2015)

Hydroxyproline and desmosine changes. Eviscerated embryos with craniofacial bones and spines were detected at E14.5. (A) The hydroxyproline assay demonstrated that the hydroxyproline content of Loxl3−/− mice compared with Loxl3+/+ mice was significantly decreased (n = 5 in each group, mean ± SD, **P < 0.01). For Loxl3+/− mice, the hydroxyproline content was also less than that of Loxl3+/+ mice (n = 5 in each group, mean ± SD,*P < 0.05). (B) The desmosine concentration was analysed by ELISA. No significant difference was found among the three groups (n = 5 in each group).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4599675&req=5

DDV333F11: Hydroxyproline and desmosine changes. Eviscerated embryos with craniofacial bones and spines were detected at E14.5. (A) The hydroxyproline assay demonstrated that the hydroxyproline content of Loxl3−/− mice compared with Loxl3+/+ mice was significantly decreased (n = 5 in each group, mean ± SD, **P < 0.01). For Loxl3+/− mice, the hydroxyproline content was also less than that of Loxl3+/+ mice (n = 5 in each group, mean ± SD,*P < 0.05). (B) The desmosine concentration was analysed by ELISA. No significant difference was found among the three groups (n = 5 in each group).
Mentions: To examine the amount of collagen fibres, a hydroxyproline assay was used to quantify the collagen content in eviscerated embryos at E14.5. The hydroxyproline content of Loxl3−/− mice was significantly less than that of Loxl3+/+ mice (P < 0.01, Fig. 11A). A decrease of the hydroxyproline content in Loxl3+/− mice was also observed (P < 0.05, Fig. 11A). We also examined the concentration of desmosine, a cross-linking amino acid of mature elastin. There was no significant difference in desmosine concentrations among the three groups (Fig. 11B).Figure 11.

Bottom Line: Failure of any one of these processes can result in embryonic malformation.We found that the obvious decrease of collagen cross-links in palate and spine that was induced by the lack of LOXL3 resulted in cleft palate and spinal deformity.The Loxl3 gene may be a candidate disease gene resulting in cleft palate and spinal deformity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, 27 Shanda Nanlu, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus