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Analysis of a Pool of Small Plasmids from Soil Heterotrophic Cultivable Bacterial Communities.

Papaleo MC, Fondi M, Maida I, Perrin E, Bevivino A, Dalmastri C, Fani R - Open Microbiol J (2015)

Bottom Line: Bacterial communities were isolated from two different sites of a 13-year experimental field with a clay-silt texture.Plasmid molecules were detected at low frequency (27 isolates, 2%) with a size ranging between 2 Kb and 40 Kb.As it might be expected, even though the viable cells title did not differ significantly between the two samplings, the overall data disclosed an uneven distribution of both species and plasmid-harboring strains.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, I-50019 Sesto Fiorentino (Florence), Italy.

ABSTRACT
In this work the analysis of the plasmid presence on soil aerobic cultivable heterotrophic bacterial communities was carried out checking a panel of 1,200 isolates, in order to establish the frequency of plasmid presence as well as the degree of plasmid flow between strains affiliated to the same or different taxon. Bacterial communities were isolated from two different sites of a 13-year experimental field with a clay-silt texture. Plasmid molecules were detected at low frequency (27 isolates, 2%) with a size ranging between 2 Kb and 40 Kb. The RAPD analysis performed on the plasmid-harboring isolates and the phylogenetic analysis of the whole community using the 16S rRNA gene sequences revealed the existence of transfer of the same plasmids between strains belonging to the same species and, in some cases, to different species of the same genus. As it might be expected, even though the viable cells title did not differ significantly between the two samplings, the overall data disclosed an uneven distribution of both species and plasmid-harboring strains.

No MeSH data available.


Schematic representation of the overall experimental strategy used in this work.
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Figure 1: Schematic representation of the overall experimental strategy used in this work.

Mentions: The plasmids ecology is still poorly understood and we know little of their distribution and diversity. In spite of the importance of plasmid molecules, an extensive analysis of the presence and frequency of plasmids in large natural cultivable microbial communities has not been performed up to now. Therefore, the aim of this work was to analyze the presence of plasmids in a large heterotrophic cultivable bacterial community isolated from soil and to check the degree of genetic flow between strains belonging to the same or different species/genus. To this purpose we used the experimental strategy schematically represented in Fig. (1).


Analysis of a Pool of Small Plasmids from Soil Heterotrophic Cultivable Bacterial Communities.

Papaleo MC, Fondi M, Maida I, Perrin E, Bevivino A, Dalmastri C, Fani R - Open Microbiol J (2015)

Schematic representation of the overall experimental strategy used in this work.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4598422&req=5

Figure 1: Schematic representation of the overall experimental strategy used in this work.
Mentions: The plasmids ecology is still poorly understood and we know little of their distribution and diversity. In spite of the importance of plasmid molecules, an extensive analysis of the presence and frequency of plasmids in large natural cultivable microbial communities has not been performed up to now. Therefore, the aim of this work was to analyze the presence of plasmids in a large heterotrophic cultivable bacterial community isolated from soil and to check the degree of genetic flow between strains belonging to the same or different species/genus. To this purpose we used the experimental strategy schematically represented in Fig. (1).

Bottom Line: Bacterial communities were isolated from two different sites of a 13-year experimental field with a clay-silt texture.Plasmid molecules were detected at low frequency (27 isolates, 2%) with a size ranging between 2 Kb and 40 Kb.As it might be expected, even though the viable cells title did not differ significantly between the two samplings, the overall data disclosed an uneven distribution of both species and plasmid-harboring strains.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, I-50019 Sesto Fiorentino (Florence), Italy.

ABSTRACT
In this work the analysis of the plasmid presence on soil aerobic cultivable heterotrophic bacterial communities was carried out checking a panel of 1,200 isolates, in order to establish the frequency of plasmid presence as well as the degree of plasmid flow between strains affiliated to the same or different taxon. Bacterial communities were isolated from two different sites of a 13-year experimental field with a clay-silt texture. Plasmid molecules were detected at low frequency (27 isolates, 2%) with a size ranging between 2 Kb and 40 Kb. The RAPD analysis performed on the plasmid-harboring isolates and the phylogenetic analysis of the whole community using the 16S rRNA gene sequences revealed the existence of transfer of the same plasmids between strains belonging to the same species and, in some cases, to different species of the same genus. As it might be expected, even though the viable cells title did not differ significantly between the two samplings, the overall data disclosed an uneven distribution of both species and plasmid-harboring strains.

No MeSH data available.