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Plasmodium falciparum Gametocyte-Specific Antibody Profiling Reveals Boosting through Natural Infection and Identifies Potential Markers of Gametocyte Exposure.

Skinner J, Huang CY, Waisberg M, Felgner PL, Doumbo OK, Ongoiba A, Kayentao K, Traore B, Crompton PD, Williamson KC - Infect. Immun. (2015)

Bottom Line: Malaria elimination efforts would benefit from vaccines that block transmission of Plasmodium falciparum gametocytes from humans to mosquitoes.Using publicly available proteomic data, we classified 91 proteins as gametocyte specific and 69 as proteins not expressed by gametocytes.In addition, IgGs specific for the gametocyte proteins Pfmdv1, Pfs16, PF3D7_1346400, and PF3D7_1024800 were detected in nearly all subjects, suggesting that seroconversion to these proteins may be a sensitive indicator of gametocyte exposure, although further studies are needed to determine the specificity and kinetics of these potential serologic markers.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA.

No MeSH data available.


Related in: MedlinePlus

Seroprevalence of GS and NGS proteins before and after the malaria season. Each symbol represents an individual antigen (194 GS and 126 NGS). Lines and whiskers represent means and standard deviations, respectively. P values determined by a linear mixed model with Bonferroni-adjusted contrasts. *, P < 0.05; ***, P < 0.001.
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Figure 3: Seroprevalence of GS and NGS proteins before and after the malaria season. Each symbol represents an individual antigen (194 GS and 126 NGS). Lines and whiskers represent means and standard deviations, respectively. P values determined by a linear mixed model with Bonferroni-adjusted contrasts. *, P < 0.05; ***, P < 0.001.

Mentions: Efforts to reduce the prevalence of gametocytes and thus malaria transmission would benefit from serologic markers that reflect gametocyte exposure (43–45). GS antigens with a high seroprevalence are likely to be sensitive indicators of gametocyte exposure and thus could be further evaluated as potential serologic markers. We defined the seroprevalence of each antigen as the percentage of plasma samples with IgG reactivity that exceeded 2 SD above the negative control. Figure 3 shows the distribution of seroprevalence for each of the GS and NGS antigens at the end of the dry season and after the malaria season. The average seroprevalence of GS proteins before the malaria season was significantly lower than the average seroprevalence of NGS proteins at the same time point (Fig. 3; GS mean before: 21.7% [SD 20.7%]; NGS mean before: 30.2% [SD 24.5%]; P = 0.01). Similarly, the average seroprevalence of GS proteins after the malaria season was significantly lower than the seroprevalence of NGS proteins at the same time point (Fig. 3; GS mean after: 30.0% [SD 26.7%]; NGS mean after: 41.2% [SD 29.8%], P < 0.001). We also found that the average seroprevalence of GS proteins increased from before to after the malaria season (P < 0.0001) (Fig. 3). Likewise, the average seroprevalence of NGS proteins increased from before to after the malaria season (P < 0.0001) (Fig. 3).


Plasmodium falciparum Gametocyte-Specific Antibody Profiling Reveals Boosting through Natural Infection and Identifies Potential Markers of Gametocyte Exposure.

Skinner J, Huang CY, Waisberg M, Felgner PL, Doumbo OK, Ongoiba A, Kayentao K, Traore B, Crompton PD, Williamson KC - Infect. Immun. (2015)

Seroprevalence of GS and NGS proteins before and after the malaria season. Each symbol represents an individual antigen (194 GS and 126 NGS). Lines and whiskers represent means and standard deviations, respectively. P values determined by a linear mixed model with Bonferroni-adjusted contrasts. *, P < 0.05; ***, P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4598406&req=5

Figure 3: Seroprevalence of GS and NGS proteins before and after the malaria season. Each symbol represents an individual antigen (194 GS and 126 NGS). Lines and whiskers represent means and standard deviations, respectively. P values determined by a linear mixed model with Bonferroni-adjusted contrasts. *, P < 0.05; ***, P < 0.001.
Mentions: Efforts to reduce the prevalence of gametocytes and thus malaria transmission would benefit from serologic markers that reflect gametocyte exposure (43–45). GS antigens with a high seroprevalence are likely to be sensitive indicators of gametocyte exposure and thus could be further evaluated as potential serologic markers. We defined the seroprevalence of each antigen as the percentage of plasma samples with IgG reactivity that exceeded 2 SD above the negative control. Figure 3 shows the distribution of seroprevalence for each of the GS and NGS antigens at the end of the dry season and after the malaria season. The average seroprevalence of GS proteins before the malaria season was significantly lower than the average seroprevalence of NGS proteins at the same time point (Fig. 3; GS mean before: 21.7% [SD 20.7%]; NGS mean before: 30.2% [SD 24.5%]; P = 0.01). Similarly, the average seroprevalence of GS proteins after the malaria season was significantly lower than the seroprevalence of NGS proteins at the same time point (Fig. 3; GS mean after: 30.0% [SD 26.7%]; NGS mean after: 41.2% [SD 29.8%], P < 0.001). We also found that the average seroprevalence of GS proteins increased from before to after the malaria season (P < 0.0001) (Fig. 3). Likewise, the average seroprevalence of NGS proteins increased from before to after the malaria season (P < 0.0001) (Fig. 3).

Bottom Line: Malaria elimination efforts would benefit from vaccines that block transmission of Plasmodium falciparum gametocytes from humans to mosquitoes.Using publicly available proteomic data, we classified 91 proteins as gametocyte specific and 69 as proteins not expressed by gametocytes.In addition, IgGs specific for the gametocyte proteins Pfmdv1, Pfs16, PF3D7_1346400, and PF3D7_1024800 were detected in nearly all subjects, suggesting that seroconversion to these proteins may be a sensitive indicator of gametocyte exposure, although further studies are needed to determine the specificity and kinetics of these potential serologic markers.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA.

No MeSH data available.


Related in: MedlinePlus