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Selected metabolites profiling of Orthosiphon stamineus Benth leaves extracts combined with chemometrics analysis and correlation with biological activities.

Saidan NH, Hamil MS, Memon AH, Abdelbari MM, Hamdan MR, Mohd KS, Majid AM, Ismail Z - BMC Complement Altern Med (2015)

Bottom Line: FTIR fingerprints combined with chemometrics showed that the extracts have been clustered based on protein and polysaccharide contents.Ten different extracts of O. stamineus have showed significant differences in the content of selected primary and secondary metabolites as well as the biological activities.Chemometric tools were able to classify and discriminate the distinctive features of extracts thus can be correlated with the biological activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Pulau Pinang, Malaysia. hafizohsaidan@yahoo.com.

ABSTRACT

Background: Studies on selected metabolites profiling of Orthosiphon stamineus extracts using chromatographic and spectroscopic techniques combined with chemometric tools have not been fully elucidated. Thus present study was performed to profile selected metabolites in O. stamineus leaves extracts using HPLC and FTIR combined with chemometric tools and correlated with biological activities.

Methods: Five different extracts were prepared using three methods; maceration, soxhlet and reflux. The extracts were analyzed using UV-Vis, HPLC and FTIR techniques. Analysis of selected primary and secondary metabolites was also evaluated. The antioxidant and cytotoxic activities of the extracts were evaluated. Chemometric tools were employed to classify the extracts based on HPLC analysis and FTIR fingerprints.

Results: The ethanolic extract using maceration characterized high content of phenolics and flavonoids, (rosmarinic acid and eupatorin) with high antioxidant activity. Ethanolic (50%) and methanolic extracts using soxhlet showed high proteins and glycosaponins. Water extracts using reflux and maceration showed high polysaccharides. Methanolic extract (50%) using soxhlet and methanolic extract using maceration showed strong cytotoxic effect against MCF7 and HCT116 cell lines, respectively. Antioxidant and cytotoxic activities showed significant correlation with selected primary and secondary metabolites. HPLC fingerprints combined with chemometrics showed the extracts have been clustered based on selected major peaks profile. FTIR fingerprints combined with chemometrics showed that the extracts have been clustered based on protein and polysaccharide contents.

Conclusion: Ten different extracts of O. stamineus have showed significant differences in the content of selected primary and secondary metabolites as well as the biological activities. Chemometric tools were able to classify and discriminate the distinctive features of extracts thus can be correlated with the biological activities.

No MeSH data available.


Related in: MedlinePlus

FTIR spectra of different extracts of O. stamineus leaves using; a maceration b soxleth and reflux (water) with principal component analysis c scores plot and d HCA dendogram
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Fig3: FTIR spectra of different extracts of O. stamineus leaves using; a maceration b soxleth and reflux (water) with principal component analysis c scores plot and d HCA dendogram

Mentions: In this study, FTIR spectra of each crude extract was recorded in IR region from 4000–600 cm−1 with 16 scans. The FTIR spectra was used to identify the functional groups in ten different crude extracts. Figure 3a and b present the FTIR spectra of ten different crude extracts extracted from three different techniques; maceration, soxhlet and reflux, respectively. In all crude extracts, the broad peak at 3550–3200 cm−1 are due to the intermolecular of hydrogen bonding. The vibration area of 2900–2800 cm−1 are assigned to the asymmetric and symmetric stretching vibration of methylene groups (CH2 and CH3), methoxy derivatives, C-H (aldehydes) including cis double bonds [9]. No peak at these region was observed for water extracts.Fig. 3


Selected metabolites profiling of Orthosiphon stamineus Benth leaves extracts combined with chemometrics analysis and correlation with biological activities.

Saidan NH, Hamil MS, Memon AH, Abdelbari MM, Hamdan MR, Mohd KS, Majid AM, Ismail Z - BMC Complement Altern Med (2015)

FTIR spectra of different extracts of O. stamineus leaves using; a maceration b soxleth and reflux (water) with principal component analysis c scores plot and d HCA dendogram
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4597610&req=5

Fig3: FTIR spectra of different extracts of O. stamineus leaves using; a maceration b soxleth and reflux (water) with principal component analysis c scores plot and d HCA dendogram
Mentions: In this study, FTIR spectra of each crude extract was recorded in IR region from 4000–600 cm−1 with 16 scans. The FTIR spectra was used to identify the functional groups in ten different crude extracts. Figure 3a and b present the FTIR spectra of ten different crude extracts extracted from three different techniques; maceration, soxhlet and reflux, respectively. In all crude extracts, the broad peak at 3550–3200 cm−1 are due to the intermolecular of hydrogen bonding. The vibration area of 2900–2800 cm−1 are assigned to the asymmetric and symmetric stretching vibration of methylene groups (CH2 and CH3), methoxy derivatives, C-H (aldehydes) including cis double bonds [9]. No peak at these region was observed for water extracts.Fig. 3

Bottom Line: FTIR fingerprints combined with chemometrics showed that the extracts have been clustered based on protein and polysaccharide contents.Ten different extracts of O. stamineus have showed significant differences in the content of selected primary and secondary metabolites as well as the biological activities.Chemometric tools were able to classify and discriminate the distinctive features of extracts thus can be correlated with the biological activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Pulau Pinang, Malaysia. hafizohsaidan@yahoo.com.

ABSTRACT

Background: Studies on selected metabolites profiling of Orthosiphon stamineus extracts using chromatographic and spectroscopic techniques combined with chemometric tools have not been fully elucidated. Thus present study was performed to profile selected metabolites in O. stamineus leaves extracts using HPLC and FTIR combined with chemometric tools and correlated with biological activities.

Methods: Five different extracts were prepared using three methods; maceration, soxhlet and reflux. The extracts were analyzed using UV-Vis, HPLC and FTIR techniques. Analysis of selected primary and secondary metabolites was also evaluated. The antioxidant and cytotoxic activities of the extracts were evaluated. Chemometric tools were employed to classify the extracts based on HPLC analysis and FTIR fingerprints.

Results: The ethanolic extract using maceration characterized high content of phenolics and flavonoids, (rosmarinic acid and eupatorin) with high antioxidant activity. Ethanolic (50%) and methanolic extracts using soxhlet showed high proteins and glycosaponins. Water extracts using reflux and maceration showed high polysaccharides. Methanolic extract (50%) using soxhlet and methanolic extract using maceration showed strong cytotoxic effect against MCF7 and HCT116 cell lines, respectively. Antioxidant and cytotoxic activities showed significant correlation with selected primary and secondary metabolites. HPLC fingerprints combined with chemometrics showed the extracts have been clustered based on selected major peaks profile. FTIR fingerprints combined with chemometrics showed that the extracts have been clustered based on protein and polysaccharide contents.

Conclusion: Ten different extracts of O. stamineus have showed significant differences in the content of selected primary and secondary metabolites as well as the biological activities. Chemometric tools were able to classify and discriminate the distinctive features of extracts thus can be correlated with the biological activities.

No MeSH data available.


Related in: MedlinePlus