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Blood plasma lipidome profile of dairy cows during the transition period.

Imhasly S, Bieli C, Naegeli H, Nyström L, Ruetten M, Gerspach C - BMC Vet. Res. (2015)

Bottom Line: Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate.The resulting data were processed by principal component analysis, revealing over 60 lipid masses that change in abundance over the test period ranging from two weeks before calving to four weeks postpartum.Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pharmacology and Toxicology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057, Zurich, Switzerland. sandro.imhasly@vetpharm.uzh.ch.

ABSTRACT

Background: The transition period of dairy cows, around parturition and the onset of lactation, involves endocrine and metabolic changes to compensate for an increased energy requirement aggravated by reduced feed intake. Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate.

Results: To study the biochemical adaptations underlying this physiologic adjustment and possible pathologic derangements, we analyzed the blood plasma lipidome of transition cows by ultra-pressure liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry. The resulting data were processed by principal component analysis, revealing over 60 lipid masses that change in abundance over the test period ranging from two weeks before calving to four weeks postpartum. Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation.

Conclusion: This newly identified shift in phospholipid composition delivers a potential biomarker to detect aberrant metabolic pathways in transition cows and also provides insights into how to prevent and treat associated disorders like fatty liver disease.

No MeSH data available.


Related in: MedlinePlus

Principal component analysis. Principal components were determined with all 579 detected masses of the 82 measured samples and arranged in 7 groups according to the time of sampling relative to the date of calving (−14 days to +28 days)
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Fig4: Principal component analysis. Principal components were determined with all 579 detected masses of the 82 measured samples and arranged in 7 groups according to the time of sampling relative to the date of calving (−14 days to +28 days)

Mentions: The lipid composition of plasma samples obtained at the different time points were compared by liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. This analysis yielded over 500 masses detected in the positive ion mode, and over 200 masses in the negative ion mode, representing lipids and other non-polar metabolites. To illustrate the differences in the lipidome profile at different stages of the transition period, from 14 days ante partum to 28 days post partum, the multivariate data were subjected to principal component analysis (PCA), which is an unsupervised clustering method that provides an overview of the results by reducing the dimensionality of complex findings. The principal components of the data acquired in the positive ion mode were graphically plotted as a score plot (Fig. 4), thus revealing that the samples from each time point cluster together forming distinguishable groups. Instead, the masses acquired in the negative ion mode did not shown any such clustering (data not shown).Fig. 4


Blood plasma lipidome profile of dairy cows during the transition period.

Imhasly S, Bieli C, Naegeli H, Nyström L, Ruetten M, Gerspach C - BMC Vet. Res. (2015)

Principal component analysis. Principal components were determined with all 579 detected masses of the 82 measured samples and arranged in 7 groups according to the time of sampling relative to the date of calving (−14 days to +28 days)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4597432&req=5

Fig4: Principal component analysis. Principal components were determined with all 579 detected masses of the 82 measured samples and arranged in 7 groups according to the time of sampling relative to the date of calving (−14 days to +28 days)
Mentions: The lipid composition of plasma samples obtained at the different time points were compared by liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. This analysis yielded over 500 masses detected in the positive ion mode, and over 200 masses in the negative ion mode, representing lipids and other non-polar metabolites. To illustrate the differences in the lipidome profile at different stages of the transition period, from 14 days ante partum to 28 days post partum, the multivariate data were subjected to principal component analysis (PCA), which is an unsupervised clustering method that provides an overview of the results by reducing the dimensionality of complex findings. The principal components of the data acquired in the positive ion mode were graphically plotted as a score plot (Fig. 4), thus revealing that the samples from each time point cluster together forming distinguishable groups. Instead, the masses acquired in the negative ion mode did not shown any such clustering (data not shown).Fig. 4

Bottom Line: Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate.The resulting data were processed by principal component analysis, revealing over 60 lipid masses that change in abundance over the test period ranging from two weeks before calving to four weeks postpartum.Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pharmacology and Toxicology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057, Zurich, Switzerland. sandro.imhasly@vetpharm.uzh.ch.

ABSTRACT

Background: The transition period of dairy cows, around parturition and the onset of lactation, involves endocrine and metabolic changes to compensate for an increased energy requirement aggravated by reduced feed intake. Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate.

Results: To study the biochemical adaptations underlying this physiologic adjustment and possible pathologic derangements, we analyzed the blood plasma lipidome of transition cows by ultra-pressure liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry. The resulting data were processed by principal component analysis, revealing over 60 lipid masses that change in abundance over the test period ranging from two weeks before calving to four weeks postpartum. Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation.

Conclusion: This newly identified shift in phospholipid composition delivers a potential biomarker to detect aberrant metabolic pathways in transition cows and also provides insights into how to prevent and treat associated disorders like fatty liver disease.

No MeSH data available.


Related in: MedlinePlus