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Blood plasma lipidome profile of dairy cows during the transition period.

Imhasly S, Bieli C, Naegeli H, Nyström L, Ruetten M, Gerspach C - BMC Vet. Res. (2015)

Bottom Line: Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate.Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation.This newly identified shift in phospholipid composition delivers a potential biomarker to detect aberrant metabolic pathways in transition cows and also provides insights into how to prevent and treat associated disorders like fatty liver disease.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pharmacology and Toxicology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057, Zurich, Switzerland. sandro.imhasly@vetpharm.uzh.ch.

ABSTRACT

Background: The transition period of dairy cows, around parturition and the onset of lactation, involves endocrine and metabolic changes to compensate for an increased energy requirement aggravated by reduced feed intake. Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate.

Results: To study the biochemical adaptations underlying this physiologic adjustment and possible pathologic derangements, we analyzed the blood plasma lipidome of transition cows by ultra-pressure liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry. The resulting data were processed by principal component analysis, revealing over 60 lipid masses that change in abundance over the test period ranging from two weeks before calving to four weeks postpartum. Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation.

Conclusion: This newly identified shift in phospholipid composition delivers a potential biomarker to detect aberrant metabolic pathways in transition cows and also provides insights into how to prevent and treat associated disorders like fatty liver disease.

No MeSH data available.


Related in: MedlinePlus

Ketone bodies. History of β-hydroxybutyrate (BHB) concentrations in the plasma of the 12 cows over the experimental period (median values with range). The time points are indicated relative to the date of calving. **P < 0.01 and ***P < 0.001 compared to β-hydroxybutyrate concentrations at the beginning of the study (day −14). ANOVA F (6,77) = 3.333, P value = 0.0057
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Fig3: Ketone bodies. History of β-hydroxybutyrate (BHB) concentrations in the plasma of the 12 cows over the experimental period (median values with range). The time points are indicated relative to the date of calving. **P < 0.01 and ***P < 0.001 compared to β-hydroxybutyrate concentrations at the beginning of the study (day −14). ANOVA F (6,77) = 3.333, P value = 0.0057

Mentions: Blood samples were obtained ante partum (on days −14 and −7) on the day of calving and post partum (on days +7, +14, +21 and +28). The blood plasma concentration of NEFAs increased from low values before calving (for example 360 ± 190 μmol/l on day −7) to significantly higher values post partum, reaching a maximum at day +7 (770 ± 160 μmol/l). These NEFA concentrations remained at high levels until at least day +28 (Fig. 2). The plasma concentrations of -hydroxybutyrate displayed some variability among the individual cows. However, a trend of increasing -hydroxybutyrate levels as the transition period progressed could be detected with significantly higher concentrations in the plasma post partum (for example 1814 ± 1407 μmol/l on day +28) compared to the samples taken ante partum (for example 632 ± 299 μmol/l on day −14) (Fig. 3).Fig. 2


Blood plasma lipidome profile of dairy cows during the transition period.

Imhasly S, Bieli C, Naegeli H, Nyström L, Ruetten M, Gerspach C - BMC Vet. Res. (2015)

Ketone bodies. History of β-hydroxybutyrate (BHB) concentrations in the plasma of the 12 cows over the experimental period (median values with range). The time points are indicated relative to the date of calving. **P < 0.01 and ***P < 0.001 compared to β-hydroxybutyrate concentrations at the beginning of the study (day −14). ANOVA F (6,77) = 3.333, P value = 0.0057
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4597432&req=5

Fig3: Ketone bodies. History of β-hydroxybutyrate (BHB) concentrations in the plasma of the 12 cows over the experimental period (median values with range). The time points are indicated relative to the date of calving. **P < 0.01 and ***P < 0.001 compared to β-hydroxybutyrate concentrations at the beginning of the study (day −14). ANOVA F (6,77) = 3.333, P value = 0.0057
Mentions: Blood samples were obtained ante partum (on days −14 and −7) on the day of calving and post partum (on days +7, +14, +21 and +28). The blood plasma concentration of NEFAs increased from low values before calving (for example 360 ± 190 μmol/l on day −7) to significantly higher values post partum, reaching a maximum at day +7 (770 ± 160 μmol/l). These NEFA concentrations remained at high levels until at least day +28 (Fig. 2). The plasma concentrations of -hydroxybutyrate displayed some variability among the individual cows. However, a trend of increasing -hydroxybutyrate levels as the transition period progressed could be detected with significantly higher concentrations in the plasma post partum (for example 1814 ± 1407 μmol/l on day +28) compared to the samples taken ante partum (for example 632 ± 299 μmol/l on day −14) (Fig. 3).Fig. 2

Bottom Line: Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate.Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation.This newly identified shift in phospholipid composition delivers a potential biomarker to detect aberrant metabolic pathways in transition cows and also provides insights into how to prevent and treat associated disorders like fatty liver disease.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pharmacology and Toxicology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057, Zurich, Switzerland. sandro.imhasly@vetpharm.uzh.ch.

ABSTRACT

Background: The transition period of dairy cows, around parturition and the onset of lactation, involves endocrine and metabolic changes to compensate for an increased energy requirement aggravated by reduced feed intake. Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate.

Results: To study the biochemical adaptations underlying this physiologic adjustment and possible pathologic derangements, we analyzed the blood plasma lipidome of transition cows by ultra-pressure liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry. The resulting data were processed by principal component analysis, revealing over 60 lipid masses that change in abundance over the test period ranging from two weeks before calving to four weeks postpartum. Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation.

Conclusion: This newly identified shift in phospholipid composition delivers a potential biomarker to detect aberrant metabolic pathways in transition cows and also provides insights into how to prevent and treat associated disorders like fatty liver disease.

No MeSH data available.


Related in: MedlinePlus