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Pulsed 980 nm short wavelength infrared neural stimulation in cochlea and laser parameter effects on auditory response characteristics.

Wang J, Lu J, Li C, Xu L, Li X, Tian L - Biomed Eng Online (2015)

Bottom Line: The results showed that the optically evoked auditory brainstem response (OABR) had a similar waveform to the acoustically induced ABR with click sound stimulus.Our study suggests that 980 nm SWIR laser is an effective stimulus for auditory neurons activation in vivo.The evoked OABR amplitude and latency are highly affected by the laser pulse energy, while not sensitive to the pulse width variability in 100-800 μs range.

View Article: PubMed Central - PubMed

Affiliation: School of Information Science and Engineering, Shandong University, 250100, Jinan, Shandong, China. 373759703@qq.com.

ABSTRACT

Background: Auditory neural stimulation with pulsed infrared radiation has been proposed as an alternative method to activate the auditory nerves in vivo. Infrared wavelengths from 1800-2150 nm with high water absorption were mainly selected in previous studies. However, few researchers have used the short-wavelength infrared (SWIR) for auditory nerve stimulation and limited pulse parameters variability has been investigated so far.

Methods: In this paper, we pioneered to use the 980 nm SWIR laser with adjustable pulse parameter as a stimulus to act on the deafened guinea pigs' cochlea in vivo. Pulsed laser light was guided through the cochlear round window to irradiate the spiral ganglion cells via a 105 μm optical fiber, and then the laser pulse parameters variability and its influence to auditory response characteristics were studied.

Results: The results showed that the optically evoked auditory brainstem response (OABR) had a similar waveform to the acoustically induced ABR with click sound stimulus. And the evoked OABR amplitude had a positive correlation, while the OABR latency period showed a negative correlation, with the laser pulse energy increase. However, when holding the laser peak power constant, the pulse width variability ranged from 100 to 800 μs showed little influence on the evoked OABR amplitude and its latency period.

Conclusions: Our study suggests that 980 nm SWIR laser is an effective stimulus for auditory neurons activation in vivo. The evoked OABR amplitude and latency are highly affected by the laser pulse energy, while not sensitive to the pulse width variability in 100-800 μs range.

No MeSH data available.


ABRs to increasing levels acoustic stimuli from 40 to 100 dB SPL with normal hearing animals. The AABR wave III peak was plotted in the traces
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Fig2: ABRs to increasing levels acoustic stimuli from 40 to 100 dB SPL with normal hearing animals. The AABR wave III peak was plotted in the traces

Mentions: Evoked AABRs under different acoustic stimulation levels were presented in Fig. 2. The AABRs all showed the classical Jewett waveform. Along with the SPL increasing, AABR intensity was strengthened, with the wave III peak amplitude from 0.64 μV at 40 dB SPL to 3.55 μV at 100 dB SPL. While after the deafening procedure, which destroyed the cochlear basilar membrane and hair cells, no detectable AABR waveform was recorded even at 100 dB stimuli. This indicated that the animals’ hearing function has already lost (shown in Fig. 3).Fig. 2


Pulsed 980 nm short wavelength infrared neural stimulation in cochlea and laser parameter effects on auditory response characteristics.

Wang J, Lu J, Li C, Xu L, Li X, Tian L - Biomed Eng Online (2015)

ABRs to increasing levels acoustic stimuli from 40 to 100 dB SPL with normal hearing animals. The AABR wave III peak was plotted in the traces
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4597400&req=5

Fig2: ABRs to increasing levels acoustic stimuli from 40 to 100 dB SPL with normal hearing animals. The AABR wave III peak was plotted in the traces
Mentions: Evoked AABRs under different acoustic stimulation levels were presented in Fig. 2. The AABRs all showed the classical Jewett waveform. Along with the SPL increasing, AABR intensity was strengthened, with the wave III peak amplitude from 0.64 μV at 40 dB SPL to 3.55 μV at 100 dB SPL. While after the deafening procedure, which destroyed the cochlear basilar membrane and hair cells, no detectable AABR waveform was recorded even at 100 dB stimuli. This indicated that the animals’ hearing function has already lost (shown in Fig. 3).Fig. 2

Bottom Line: The results showed that the optically evoked auditory brainstem response (OABR) had a similar waveform to the acoustically induced ABR with click sound stimulus.Our study suggests that 980 nm SWIR laser is an effective stimulus for auditory neurons activation in vivo.The evoked OABR amplitude and latency are highly affected by the laser pulse energy, while not sensitive to the pulse width variability in 100-800 μs range.

View Article: PubMed Central - PubMed

Affiliation: School of Information Science and Engineering, Shandong University, 250100, Jinan, Shandong, China. 373759703@qq.com.

ABSTRACT

Background: Auditory neural stimulation with pulsed infrared radiation has been proposed as an alternative method to activate the auditory nerves in vivo. Infrared wavelengths from 1800-2150 nm with high water absorption were mainly selected in previous studies. However, few researchers have used the short-wavelength infrared (SWIR) for auditory nerve stimulation and limited pulse parameters variability has been investigated so far.

Methods: In this paper, we pioneered to use the 980 nm SWIR laser with adjustable pulse parameter as a stimulus to act on the deafened guinea pigs' cochlea in vivo. Pulsed laser light was guided through the cochlear round window to irradiate the spiral ganglion cells via a 105 μm optical fiber, and then the laser pulse parameters variability and its influence to auditory response characteristics were studied.

Results: The results showed that the optically evoked auditory brainstem response (OABR) had a similar waveform to the acoustically induced ABR with click sound stimulus. And the evoked OABR amplitude had a positive correlation, while the OABR latency period showed a negative correlation, with the laser pulse energy increase. However, when holding the laser peak power constant, the pulse width variability ranged from 100 to 800 μs showed little influence on the evoked OABR amplitude and its latency period.

Conclusions: Our study suggests that 980 nm SWIR laser is an effective stimulus for auditory neurons activation in vivo. The evoked OABR amplitude and latency are highly affected by the laser pulse energy, while not sensitive to the pulse width variability in 100-800 μs range.

No MeSH data available.