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Interaction between microRNA expression and classical risk factors in the risk of coronary heart disease.

Ding XQ, Ge PC, Liu Z, Jia H, Chen X, An FH, Li LH, Chen ZH, Mao HW, Li ZY, Gu Y, Zhu TB, Li CJ, Wang LS, Ma WZ, Yang ZJ, Jia EZ - Sci Rep (2015)

Bottom Line: The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037).Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017).We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

ABSTRACT
The aim of this study was to identify the synergistic effect of microRNA expression with classical risk factors of coronary heart disease (CHD) and to explore their diagnostic value for coronary stenotic lesions in subjects with CHD. Plasma samples were obtained from 66 subjects with CHD and from 58 control individuals. A quantitative reverse-transcription PCR (RT-qPCR) assay was conducted to confirm the relative expressions of the known CHD-related miRNAs. The severity of coronary atherosclerosis was based on the Gensini scoring system. The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037). Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017). Of all the miRNAs, miR-125b showed the lowest AUC (0.405; 95% CI: 0.305 ~ 0.506, p = 0.070). We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%. Therefore, miR-125b was shown to play an important role in individual's susceptibility to developing CHD.

No MeSH data available.


Related in: MedlinePlus

The receiver operating characteristic curve of miR-125b for the ability to differentiate the CHD cases from the control individuals, AUC (95% CI) was 0.405(0.305 ~ 0.506).
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f8: The receiver operating characteristic curve of miR-125b for the ability to differentiate the CHD cases from the control individuals, AUC (95% CI) was 0.405(0.305 ~ 0.506).

Mentions: To further explore the applicability of circulating miRNAs and classical risk factors as potential diagnostic biomarkers of CHD, subsequent ROC analyses were performed, and the results are shown in Table 4. The AUC was 0.695 for age (95% CI: 0.601 ~ 0.788, p = 0.000) (Fig. 4); 0.354 for HDL-C (95% CI: 0.255 ~ 0.454, p = 0.006) (Fig. 5); 0.647 for FBG (95% CI: 0.549 ~ 0.745, p = 0.006) (Fig. 6); and 0.622 for CR (95% CI: 0.522 ~ 0.723, p = 0.021) (Fig. 7). Of all the miRNAs, miR-125b showed the lowest AUC (0.405; 95% CI: 0.305 ~ 0.506, p = 0.070) (Fig. 8). Due to the levels of HDL-C, miR-133b and miR-125b were lower in patients with CHD than in the controls; the AUC of the above three variables were less than 0.5. The optimal cut-off value, the sensitivity, the specificity and Youden index of age, CR, FBG, HDL-C, and miR-125b are shown in Table 5.


Interaction between microRNA expression and classical risk factors in the risk of coronary heart disease.

Ding XQ, Ge PC, Liu Z, Jia H, Chen X, An FH, Li LH, Chen ZH, Mao HW, Li ZY, Gu Y, Zhu TB, Li CJ, Wang LS, Ma WZ, Yang ZJ, Jia EZ - Sci Rep (2015)

The receiver operating characteristic curve of miR-125b for the ability to differentiate the CHD cases from the control individuals, AUC (95% CI) was 0.405(0.305 ~ 0.506).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4597355&req=5

f8: The receiver operating characteristic curve of miR-125b for the ability to differentiate the CHD cases from the control individuals, AUC (95% CI) was 0.405(0.305 ~ 0.506).
Mentions: To further explore the applicability of circulating miRNAs and classical risk factors as potential diagnostic biomarkers of CHD, subsequent ROC analyses were performed, and the results are shown in Table 4. The AUC was 0.695 for age (95% CI: 0.601 ~ 0.788, p = 0.000) (Fig. 4); 0.354 for HDL-C (95% CI: 0.255 ~ 0.454, p = 0.006) (Fig. 5); 0.647 for FBG (95% CI: 0.549 ~ 0.745, p = 0.006) (Fig. 6); and 0.622 for CR (95% CI: 0.522 ~ 0.723, p = 0.021) (Fig. 7). Of all the miRNAs, miR-125b showed the lowest AUC (0.405; 95% CI: 0.305 ~ 0.506, p = 0.070) (Fig. 8). Due to the levels of HDL-C, miR-133b and miR-125b were lower in patients with CHD than in the controls; the AUC of the above three variables were less than 0.5. The optimal cut-off value, the sensitivity, the specificity and Youden index of age, CR, FBG, HDL-C, and miR-125b are shown in Table 5.

Bottom Line: The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037).Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017).We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

ABSTRACT
The aim of this study was to identify the synergistic effect of microRNA expression with classical risk factors of coronary heart disease (CHD) and to explore their diagnostic value for coronary stenotic lesions in subjects with CHD. Plasma samples were obtained from 66 subjects with CHD and from 58 control individuals. A quantitative reverse-transcription PCR (RT-qPCR) assay was conducted to confirm the relative expressions of the known CHD-related miRNAs. The severity of coronary atherosclerosis was based on the Gensini scoring system. The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037). Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017). Of all the miRNAs, miR-125b showed the lowest AUC (0.405; 95% CI: 0.305 ~ 0.506, p = 0.070). We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%. Therefore, miR-125b was shown to play an important role in individual's susceptibility to developing CHD.

No MeSH data available.


Related in: MedlinePlus