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Interaction between microRNA expression and classical risk factors in the risk of coronary heart disease.

Ding XQ, Ge PC, Liu Z, Jia H, Chen X, An FH, Li LH, Chen ZH, Mao HW, Li ZY, Gu Y, Zhu TB, Li CJ, Wang LS, Ma WZ, Yang ZJ, Jia EZ - Sci Rep (2015)

Bottom Line: The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037).Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017).We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

ABSTRACT
The aim of this study was to identify the synergistic effect of microRNA expression with classical risk factors of coronary heart disease (CHD) and to explore their diagnostic value for coronary stenotic lesions in subjects with CHD. Plasma samples were obtained from 66 subjects with CHD and from 58 control individuals. A quantitative reverse-transcription PCR (RT-qPCR) assay was conducted to confirm the relative expressions of the known CHD-related miRNAs. The severity of coronary atherosclerosis was based on the Gensini scoring system. The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037). Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017). Of all the miRNAs, miR-125b showed the lowest AUC (0.405; 95% CI: 0.305 ~ 0.506, p = 0.070). We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%. Therefore, miR-125b was shown to play an important role in individual's susceptibility to developing CHD.

No MeSH data available.


Related in: MedlinePlus

The heatmap of real-time PCR expression profiling of microRNA in subjects with CHD and controls.
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f3: The heatmap of real-time PCR expression profiling of microRNA in subjects with CHD and controls.

Mentions: Table 3 shows the results of Spearman correlation analyses between Gensini score, clinical characteristics, and miRNAs. The results indicate that the Gensini score was positively associated with age (r = 0.339, p = 0.000), FBG (r = 0.223, p = 0.014), and CR (r = 0.276, p = 0.002). A negative association was found between the Gensini score and HDL-C (r = −0.211, p = 0.021), and miR-125b (r = −0.215, p = 0.017). And, the heatmap was generated by transformation of the real-time PCR data presented as 2−Δct and was shown in Fig. 3.


Interaction between microRNA expression and classical risk factors in the risk of coronary heart disease.

Ding XQ, Ge PC, Liu Z, Jia H, Chen X, An FH, Li LH, Chen ZH, Mao HW, Li ZY, Gu Y, Zhu TB, Li CJ, Wang LS, Ma WZ, Yang ZJ, Jia EZ - Sci Rep (2015)

The heatmap of real-time PCR expression profiling of microRNA in subjects with CHD and controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4597355&req=5

f3: The heatmap of real-time PCR expression profiling of microRNA in subjects with CHD and controls.
Mentions: Table 3 shows the results of Spearman correlation analyses between Gensini score, clinical characteristics, and miRNAs. The results indicate that the Gensini score was positively associated with age (r = 0.339, p = 0.000), FBG (r = 0.223, p = 0.014), and CR (r = 0.276, p = 0.002). A negative association was found between the Gensini score and HDL-C (r = −0.211, p = 0.021), and miR-125b (r = −0.215, p = 0.017). And, the heatmap was generated by transformation of the real-time PCR data presented as 2−Δct and was shown in Fig. 3.

Bottom Line: The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037).Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017).We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

ABSTRACT
The aim of this study was to identify the synergistic effect of microRNA expression with classical risk factors of coronary heart disease (CHD) and to explore their diagnostic value for coronary stenotic lesions in subjects with CHD. Plasma samples were obtained from 66 subjects with CHD and from 58 control individuals. A quantitative reverse-transcription PCR (RT-qPCR) assay was conducted to confirm the relative expressions of the known CHD-related miRNAs. The severity of coronary atherosclerosis was based on the Gensini scoring system. The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037). Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017). Of all the miRNAs, miR-125b showed the lowest AUC (0.405; 95% CI: 0.305 ~ 0.506, p = 0.070). We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%. Therefore, miR-125b was shown to play an important role in individual's susceptibility to developing CHD.

No MeSH data available.


Related in: MedlinePlus