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Molecular Mechanisms for High Hydrostatic Pressure-Induced Wing Mutagenesis in Drosophila melanogaster.

Wang H, Wang K, Xiao G, Ma J, Wang B, Shen S, Fu X, Zou G, Zou B - Sci Rep (2015)

Bottom Line: Abnormal wing phenotypes of D. melanogaster induced by HHP were used to investigate the mutagenic mechanisms of HHP on organism.Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR.This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.

ABSTRACT
Although High hydrostatic pressure (HHP) as an important physical and chemical tool has been increasingly applied to research of organism, the response mechanisms of organism to HHP have not been elucidated clearly thus far. To identify mutagenic mechanisms of HHP on organisms, here, we treated Drosophila melanogaster (D. melanogaster) eggs with HHP. Approximately 75% of the surviving flies showed significant morphological abnormalities from the egg to the adult stages compared with control flies (p < 0.05). Some eggs displayed abnormal chorionic appendages, some larvae were large and red, and some adult flies showed wing abnormalities. Abnormal wing phenotypes of D. melanogaster induced by HHP were used to investigate the mutagenic mechanisms of HHP on organism. Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR. We also compared wing development-related central genes in the mutant flies with control flies using DNA sequencing to show two point mutations in the vestigial (vg) gene. This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.

No MeSH data available.


Related in: MedlinePlus

The mutant phenotypes of D. melanogaster with HHP induction.(a) D. melanogaster in the absence of HHP treatment. Normally, the wing is oval shaped, with a length twice that of the abdomen, normal shape and established margin. (b–f) HHP-induced mutants show wing degradation and obvious modifications in wing size, shape, and margin formation.
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f2: The mutant phenotypes of D. melanogaster with HHP induction.(a) D. melanogaster in the absence of HHP treatment. Normally, the wing is oval shaped, with a length twice that of the abdomen, normal shape and established margin. (b–f) HHP-induced mutants show wing degradation and obvious modifications in wing size, shape, and margin formation.

Mentions: Figure 1 shows the wing morphs induced by HHP in D. melanogaster. To determine whether HHP induced mutagenic effects in D. melanogaster, we treated D. melanogaster eggs using a pressure of 60 MPa for 20 min at room temperature. Approximately 75% of the flies that survived the HHP treatment showed significant morphological abnormalities from the egg stage to the adult stage compared with control flies (p < 0.05). The eggs displayed abnormal chorionic appendages, the larvae were large and red, the pupae had melanotic tumors, and the adult flies showed wing abnormalities. Normally, the wing is oval shaped, with a length two times than that of the abdomen and five longitudinal veins and two cross veins as well as established wing margins. However, the abnormal wings of D. melanogaster treated with HHP showed degradation and obvious modifications in size, shape, vein patterning, and margin formation (Fig. 1), resulting in an inability to fly. Thus, wing mutant phenotypes were selected for investigating the mechanisms of HHP-induced mutagenesis (Fig. 2).


Molecular Mechanisms for High Hydrostatic Pressure-Induced Wing Mutagenesis in Drosophila melanogaster.

Wang H, Wang K, Xiao G, Ma J, Wang B, Shen S, Fu X, Zou G, Zou B - Sci Rep (2015)

The mutant phenotypes of D. melanogaster with HHP induction.(a) D. melanogaster in the absence of HHP treatment. Normally, the wing is oval shaped, with a length twice that of the abdomen, normal shape and established margin. (b–f) HHP-induced mutants show wing degradation and obvious modifications in wing size, shape, and margin formation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4597337&req=5

f2: The mutant phenotypes of D. melanogaster with HHP induction.(a) D. melanogaster in the absence of HHP treatment. Normally, the wing is oval shaped, with a length twice that of the abdomen, normal shape and established margin. (b–f) HHP-induced mutants show wing degradation and obvious modifications in wing size, shape, and margin formation.
Mentions: Figure 1 shows the wing morphs induced by HHP in D. melanogaster. To determine whether HHP induced mutagenic effects in D. melanogaster, we treated D. melanogaster eggs using a pressure of 60 MPa for 20 min at room temperature. Approximately 75% of the flies that survived the HHP treatment showed significant morphological abnormalities from the egg stage to the adult stage compared with control flies (p < 0.05). The eggs displayed abnormal chorionic appendages, the larvae were large and red, the pupae had melanotic tumors, and the adult flies showed wing abnormalities. Normally, the wing is oval shaped, with a length two times than that of the abdomen and five longitudinal veins and two cross veins as well as established wing margins. However, the abnormal wings of D. melanogaster treated with HHP showed degradation and obvious modifications in size, shape, vein patterning, and margin formation (Fig. 1), resulting in an inability to fly. Thus, wing mutant phenotypes were selected for investigating the mechanisms of HHP-induced mutagenesis (Fig. 2).

Bottom Line: Abnormal wing phenotypes of D. melanogaster induced by HHP were used to investigate the mutagenic mechanisms of HHP on organism.Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR.This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.

ABSTRACT
Although High hydrostatic pressure (HHP) as an important physical and chemical tool has been increasingly applied to research of organism, the response mechanisms of organism to HHP have not been elucidated clearly thus far. To identify mutagenic mechanisms of HHP on organisms, here, we treated Drosophila melanogaster (D. melanogaster) eggs with HHP. Approximately 75% of the surviving flies showed significant morphological abnormalities from the egg to the adult stages compared with control flies (p < 0.05). Some eggs displayed abnormal chorionic appendages, some larvae were large and red, and some adult flies showed wing abnormalities. Abnormal wing phenotypes of D. melanogaster induced by HHP were used to investigate the mutagenic mechanisms of HHP on organism. Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR. We also compared wing development-related central genes in the mutant flies with control flies using DNA sequencing to show two point mutations in the vestigial (vg) gene. This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.

No MeSH data available.


Related in: MedlinePlus