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Molecular Mechanisms for High Hydrostatic Pressure-Induced Wing Mutagenesis in Drosophila melanogaster.

Wang H, Wang K, Xiao G, Ma J, Wang B, Shen S, Fu X, Zou G, Zou B - Sci Rep (2015)

Bottom Line: Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR.We also compared wing development-related central genes in the mutant flies with control flies using DNA sequencing to show two point mutations in the vestigial (vg) gene.This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.

ABSTRACT
Although High hydrostatic pressure (HHP) as an important physical and chemical tool has been increasingly applied to research of organism, the response mechanisms of organism to HHP have not been elucidated clearly thus far. To identify mutagenic mechanisms of HHP on organisms, here, we treated Drosophila melanogaster (D. melanogaster) eggs with HHP. Approximately 75% of the surviving flies showed significant morphological abnormalities from the egg to the adult stages compared with control flies (p < 0.05). Some eggs displayed abnormal chorionic appendages, some larvae were large and red, and some adult flies showed wing abnormalities. Abnormal wing phenotypes of D. melanogaster induced by HHP were used to investigate the mutagenic mechanisms of HHP on organism. Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR. We also compared wing development-related central genes in the mutant flies with control flies using DNA sequencing to show two point mutations in the vestigial (vg) gene. This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.

No MeSH data available.


Related in: MedlinePlus

Abnormal wing phenotypes of Drosophila melanogaster (D. melanogaster) induced by high hydrostatic pressure (HHP).(a) The wing shape of D. melanogaster in the absence of HHP treatment. Wings in control D. melanogaster have five longitudinal veins (L1–L5, representing the first through the fifth longitudinal veins, respectively) and two cross veins (C1 and C2, representing the first and second cross veins, respectively) as well as established wing margins. (b–l) Abnormal wings in D. melanogaster induced by HHP treatment. The images show the degradation and modifications in wing size, shape, vein patterning, and margin formation.
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f1: Abnormal wing phenotypes of Drosophila melanogaster (D. melanogaster) induced by high hydrostatic pressure (HHP).(a) The wing shape of D. melanogaster in the absence of HHP treatment. Wings in control D. melanogaster have five longitudinal veins (L1–L5, representing the first through the fifth longitudinal veins, respectively) and two cross veins (C1 and C2, representing the first and second cross veins, respectively) as well as established wing margins. (b–l) Abnormal wings in D. melanogaster induced by HHP treatment. The images show the degradation and modifications in wing size, shape, vein patterning, and margin formation.

Mentions: Figure 1 shows the wing morphs induced by HHP in D. melanogaster. To determine whether HHP induced mutagenic effects in D. melanogaster, we treated D. melanogaster eggs using a pressure of 60 MPa for 20 min at room temperature. Approximately 75% of the flies that survived the HHP treatment showed significant morphological abnormalities from the egg stage to the adult stage compared with control flies (p < 0.05). The eggs displayed abnormal chorionic appendages, the larvae were large and red, the pupae had melanotic tumors, and the adult flies showed wing abnormalities. Normally, the wing is oval shaped, with a length two times than that of the abdomen and five longitudinal veins and two cross veins as well as established wing margins. However, the abnormal wings of D. melanogaster treated with HHP showed degradation and obvious modifications in size, shape, vein patterning, and margin formation (Fig. 1), resulting in an inability to fly. Thus, wing mutant phenotypes were selected for investigating the mechanisms of HHP-induced mutagenesis (Fig. 2).


Molecular Mechanisms for High Hydrostatic Pressure-Induced Wing Mutagenesis in Drosophila melanogaster.

Wang H, Wang K, Xiao G, Ma J, Wang B, Shen S, Fu X, Zou G, Zou B - Sci Rep (2015)

Abnormal wing phenotypes of Drosophila melanogaster (D. melanogaster) induced by high hydrostatic pressure (HHP).(a) The wing shape of D. melanogaster in the absence of HHP treatment. Wings in control D. melanogaster have five longitudinal veins (L1–L5, representing the first through the fifth longitudinal veins, respectively) and two cross veins (C1 and C2, representing the first and second cross veins, respectively) as well as established wing margins. (b–l) Abnormal wings in D. melanogaster induced by HHP treatment. The images show the degradation and modifications in wing size, shape, vein patterning, and margin formation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4597337&req=5

f1: Abnormal wing phenotypes of Drosophila melanogaster (D. melanogaster) induced by high hydrostatic pressure (HHP).(a) The wing shape of D. melanogaster in the absence of HHP treatment. Wings in control D. melanogaster have five longitudinal veins (L1–L5, representing the first through the fifth longitudinal veins, respectively) and two cross veins (C1 and C2, representing the first and second cross veins, respectively) as well as established wing margins. (b–l) Abnormal wings in D. melanogaster induced by HHP treatment. The images show the degradation and modifications in wing size, shape, vein patterning, and margin formation.
Mentions: Figure 1 shows the wing morphs induced by HHP in D. melanogaster. To determine whether HHP induced mutagenic effects in D. melanogaster, we treated D. melanogaster eggs using a pressure of 60 MPa for 20 min at room temperature. Approximately 75% of the flies that survived the HHP treatment showed significant morphological abnormalities from the egg stage to the adult stage compared with control flies (p < 0.05). The eggs displayed abnormal chorionic appendages, the larvae were large and red, the pupae had melanotic tumors, and the adult flies showed wing abnormalities. Normally, the wing is oval shaped, with a length two times than that of the abdomen and five longitudinal veins and two cross veins as well as established wing margins. However, the abnormal wings of D. melanogaster treated with HHP showed degradation and obvious modifications in size, shape, vein patterning, and margin formation (Fig. 1), resulting in an inability to fly. Thus, wing mutant phenotypes were selected for investigating the mechanisms of HHP-induced mutagenesis (Fig. 2).

Bottom Line: Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR.We also compared wing development-related central genes in the mutant flies with control flies using DNA sequencing to show two point mutations in the vestigial (vg) gene.This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.

ABSTRACT
Although High hydrostatic pressure (HHP) as an important physical and chemical tool has been increasingly applied to research of organism, the response mechanisms of organism to HHP have not been elucidated clearly thus far. To identify mutagenic mechanisms of HHP on organisms, here, we treated Drosophila melanogaster (D. melanogaster) eggs with HHP. Approximately 75% of the surviving flies showed significant morphological abnormalities from the egg to the adult stages compared with control flies (p < 0.05). Some eggs displayed abnormal chorionic appendages, some larvae were large and red, and some adult flies showed wing abnormalities. Abnormal wing phenotypes of D. melanogaster induced by HHP were used to investigate the mutagenic mechanisms of HHP on organism. Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR. We also compared wing development-related central genes in the mutant flies with control flies using DNA sequencing to show two point mutations in the vestigial (vg) gene. This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.

No MeSH data available.


Related in: MedlinePlus