Limits...
Integrated transcriptome sequencing and dynamic analysis reveal carbon source partitioning between terpenoid and oil accumulation in developing Lindera glauca fruits.

Niu J, Chen Y, An J, Hou X, Cai J, Wang J, Zhang Z, Lin S - Sci Rep (2015)

Bottom Line: By Illumina sequencing, the obtained approximately 81 million reads are assembled into 69,160 unigenes, among which 174, 71, 81 and 155 unigenes are implicated in glycolysis, pentose phosphate pathway (PPP), TBP and OBP, respectively.Integrated differential expression profiling and qRT-PCR, we specifically characterize the key enzymes and transcription factors (TFs) involved in regulating carbon allocation ratios for terpenoid or oil accumulation in developing LGF.These results contribute to our understanding of the regulatory mechanisms of carbon source partitioning between terpenoid and oil in developing LGF, and to the improvement of resource utilization and molecular breeding for L. glauca.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.

ABSTRACT
Lindera glauca fruits (LGF) with the abundance of terpenoid and oil has emerged as a novel specific material for industrial and medicinal application in China, but the complex regulatory mechanisms of carbon source partitioning into terpenoid biosynthetic pathway (TBP) and oil biosynthetic pathway (OBP) in developing LGF is still unknown. Here we perform the analysis of contents and compositions of terpenoid and oil from 7 stages of developing LGF to characterize a dramatic difference in temporal accumulative patterns. The resulting 3 crucial samples at 50, 125 and 150 days after flowering (DAF) were selected for comparative deep transcriptome analysis. By Illumina sequencing, the obtained approximately 81 million reads are assembled into 69,160 unigenes, among which 174, 71, 81 and 155 unigenes are implicated in glycolysis, pentose phosphate pathway (PPP), TBP and OBP, respectively. Integrated differential expression profiling and qRT-PCR, we specifically characterize the key enzymes and transcription factors (TFs) involved in regulating carbon allocation ratios for terpenoid or oil accumulation in developing LGF. These results contribute to our understanding of the regulatory mechanisms of carbon source partitioning between terpenoid and oil in developing LGF, and to the improvement of resource utilization and molecular breeding for L. glauca.

No MeSH data available.


Temporal profile of transcriptional levels for enzymes involved in FA synthesis and TAG assembly.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4597268&req=5

f6: Temporal profile of transcriptional levels for enzymes involved in FA synthesis and TAG assembly.

Mentions: In middle-late development of LGF, a greater proportion of hexose to acetyl-CoA flux is used for oil accumulation (Fig. 5). Indeed, the majority of our annotated genes associated with de novo FA synthesis were identified with a higher transcriptional level in middle-late development of LGF (Fig. 5 and Supplementary Table S7). It is, therefore, concluded that strongly increased FA synthesis, together with plastid carbon supply, is crucial for the higher oil accumulation in developing LGF. Notably, we characterized 2 genes for two BCCP isoforms (BCCP1 and BCCP2), but the abundance of BCCP1 expression was 3-fold higher than BCCP2 (Fig. 6), indicating a more important contribution of BCCP1subunit to control the carbon flux of acetyl-CoA to malonyl-CoA regulated by ACCase activity for de novo FA biosynthesis in developing LGF. The produced FA-ACP (16:0-, 18:0- and 18:1-ACP) must be hydrolyzed by FATB or FATA to release free FAs (saturated and monounsaturated) that are exported from the plastid required for TAG biosynthesis26. Unexpectedly, we only identified four unigenes encoding two FATB isozymes (FATB1 and FATB2) but no unigenes similar to FATA (Supplementary Table S7). Moreover, the analysis of transcriptional profiles revealed a gradual increased expression for FATB1 in developing LGF, and a notably up-regulated expression for FATB2 only at 125 DAF (comparable to expression pattern of SAD6), as was strongly supported by our qRT-PCR results of their expression alterations in 7 developing stages (Fig. 6 and Supplementary Fig. S5). Thus, we concluded that FATB1 and FATB2 are implicated respectively in regulation the flux of saturated (C16:0 and C18:0) and monounsaturated (C18:1) FAs from plastid to cytosol for TAG biosynthesis in developing LGF, which was also strengthened by our observation on palmitic (C16:0) and oleic acid (C18:1) as the major FA compositions (Table 1).


Integrated transcriptome sequencing and dynamic analysis reveal carbon source partitioning between terpenoid and oil accumulation in developing Lindera glauca fruits.

Niu J, Chen Y, An J, Hou X, Cai J, Wang J, Zhang Z, Lin S - Sci Rep (2015)

Temporal profile of transcriptional levels for enzymes involved in FA synthesis and TAG assembly.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4597268&req=5

f6: Temporal profile of transcriptional levels for enzymes involved in FA synthesis and TAG assembly.
Mentions: In middle-late development of LGF, a greater proportion of hexose to acetyl-CoA flux is used for oil accumulation (Fig. 5). Indeed, the majority of our annotated genes associated with de novo FA synthesis were identified with a higher transcriptional level in middle-late development of LGF (Fig. 5 and Supplementary Table S7). It is, therefore, concluded that strongly increased FA synthesis, together with plastid carbon supply, is crucial for the higher oil accumulation in developing LGF. Notably, we characterized 2 genes for two BCCP isoforms (BCCP1 and BCCP2), but the abundance of BCCP1 expression was 3-fold higher than BCCP2 (Fig. 6), indicating a more important contribution of BCCP1subunit to control the carbon flux of acetyl-CoA to malonyl-CoA regulated by ACCase activity for de novo FA biosynthesis in developing LGF. The produced FA-ACP (16:0-, 18:0- and 18:1-ACP) must be hydrolyzed by FATB or FATA to release free FAs (saturated and monounsaturated) that are exported from the plastid required for TAG biosynthesis26. Unexpectedly, we only identified four unigenes encoding two FATB isozymes (FATB1 and FATB2) but no unigenes similar to FATA (Supplementary Table S7). Moreover, the analysis of transcriptional profiles revealed a gradual increased expression for FATB1 in developing LGF, and a notably up-regulated expression for FATB2 only at 125 DAF (comparable to expression pattern of SAD6), as was strongly supported by our qRT-PCR results of their expression alterations in 7 developing stages (Fig. 6 and Supplementary Fig. S5). Thus, we concluded that FATB1 and FATB2 are implicated respectively in regulation the flux of saturated (C16:0 and C18:0) and monounsaturated (C18:1) FAs from plastid to cytosol for TAG biosynthesis in developing LGF, which was also strengthened by our observation on palmitic (C16:0) and oleic acid (C18:1) as the major FA compositions (Table 1).

Bottom Line: By Illumina sequencing, the obtained approximately 81 million reads are assembled into 69,160 unigenes, among which 174, 71, 81 and 155 unigenes are implicated in glycolysis, pentose phosphate pathway (PPP), TBP and OBP, respectively.Integrated differential expression profiling and qRT-PCR, we specifically characterize the key enzymes and transcription factors (TFs) involved in regulating carbon allocation ratios for terpenoid or oil accumulation in developing LGF.These results contribute to our understanding of the regulatory mechanisms of carbon source partitioning between terpenoid and oil in developing LGF, and to the improvement of resource utilization and molecular breeding for L. glauca.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.

ABSTRACT
Lindera glauca fruits (LGF) with the abundance of terpenoid and oil has emerged as a novel specific material for industrial and medicinal application in China, but the complex regulatory mechanisms of carbon source partitioning into terpenoid biosynthetic pathway (TBP) and oil biosynthetic pathway (OBP) in developing LGF is still unknown. Here we perform the analysis of contents and compositions of terpenoid and oil from 7 stages of developing LGF to characterize a dramatic difference in temporal accumulative patterns. The resulting 3 crucial samples at 50, 125 and 150 days after flowering (DAF) were selected for comparative deep transcriptome analysis. By Illumina sequencing, the obtained approximately 81 million reads are assembled into 69,160 unigenes, among which 174, 71, 81 and 155 unigenes are implicated in glycolysis, pentose phosphate pathway (PPP), TBP and OBP, respectively. Integrated differential expression profiling and qRT-PCR, we specifically characterize the key enzymes and transcription factors (TFs) involved in regulating carbon allocation ratios for terpenoid or oil accumulation in developing LGF. These results contribute to our understanding of the regulatory mechanisms of carbon source partitioning between terpenoid and oil in developing LGF, and to the improvement of resource utilization and molecular breeding for L. glauca.

No MeSH data available.