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Forebrain-Specific Loss of BMPRII in Mice Reduces Anxiety and Increases Object Exploration.

McBrayer ZL, Dimova J, Pisansky MT, Sun M, Beppu H, Gewirtz JC, O'Connor MB - PLoS ONE (2015)

Bottom Line: We found that BMPRII mutant mice had normal spatial learning and memory in the Morris water maze, but showed significantly reduced swimming speeds with increased floating behavior.In the Elevated Plus Maze, BMPRII mutants and Smad4 mutants showed reduced anxiety, while in exploratory tests, BMPRII mutants showed more interest in object exploration.These results suggest that loss of BMPRII in the mouse hippocampus and forebrain does not disrupt spatial learning and memory encoding, but instead impacts exploratory and anxiety-related behaviors.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America.

ABSTRACT
To investigate the role of Bone Morphogenic Protein Receptor Type II (BMPRII) in learning, memory, and exploratory behavior in mice, a tissue-specific knockout of BMPRII in the post-natal hippocampus and forebrain was generated. We found that BMPRII mutant mice had normal spatial learning and memory in the Morris water maze, but showed significantly reduced swimming speeds with increased floating behavior. Further analysis using the Porsolt Swim Test to investigate behavioral despair did not reveal any differences in immobility between mutants and controls. In the Elevated Plus Maze, BMPRII mutants and Smad4 mutants showed reduced anxiety, while in exploratory tests, BMPRII mutants showed more interest in object exploration. These results suggest that loss of BMPRII in the mouse hippocampus and forebrain does not disrupt spatial learning and memory encoding, but instead impacts exploratory and anxiety-related behaviors.

No MeSH data available.


Related in: MedlinePlus

Spatial Learning and Memory in the MWM is Not Affected by Loss of BMPRII.(A) fbΔBMPRII mutant mice (red) do not have a significantly different learning curve than control littermates (black) during the cued (1) or first hidden (2–10) phases of the water maze, but show a slower learning curve during the reversal (12–16) phase of the water maze. (B) The swimming distance of the mice was not different than control littermates, (C) but the swimming speed of the mice was significantly slower on most days of the water maze. The appearance of a slower learning trend during the first hidden phase and reversal is likely an artifact of slow swimming speed rather than slower learning. (D-E) During probe trials fbΔBMPRII mutant mice show similar preference for the target quadrant as control littermates during both the first hidden phase and the reversal phase. Abbreviations: (P) platform position, (Cu) former cued platform position, (F) former hidden platform position. Results reported as mean ± S.E.M. Asterisk indicates *p<0.05, **p<0.005, ***p<0.0005, ****p<0.00005.
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pone.0139860.g005: Spatial Learning and Memory in the MWM is Not Affected by Loss of BMPRII.(A) fbΔBMPRII mutant mice (red) do not have a significantly different learning curve than control littermates (black) during the cued (1) or first hidden (2–10) phases of the water maze, but show a slower learning curve during the reversal (12–16) phase of the water maze. (B) The swimming distance of the mice was not different than control littermates, (C) but the swimming speed of the mice was significantly slower on most days of the water maze. The appearance of a slower learning trend during the first hidden phase and reversal is likely an artifact of slow swimming speed rather than slower learning. (D-E) During probe trials fbΔBMPRII mutant mice show similar preference for the target quadrant as control littermates during both the first hidden phase and the reversal phase. Abbreviations: (P) platform position, (Cu) former cued platform position, (F) former hidden platform position. Results reported as mean ± S.E.M. Asterisk indicates *p<0.05, **p<0.005, ***p<0.0005, ****p<0.00005.

Mentions: During the cued phase of the MWM on day 1, both fbΔBMPRII mutants and control littermates showed similar escape latencies. On days 2–10 of the first hidden platform phase, fbΔBMPRII mutants showed a tendency for slower escape latencies compared to control littermates, but there was no significant effect of genotype (F(1,30) = 3.044, p = 0.0913). During the reversal phase of the hidden platform, fbΔBMPRII mutants showed a slower escape latency that was much more pronounced than during the first hidden phase. A repeated measures two-way ANOVA revealed a significant effect of genotype (F(1,30) = 6.183, p = 0.0187). On the first day of the reversal, mutants and controls showed similar escape latencies, but on the second day of the reversal, fbΔBMPRII mutants took significantly longer to locate the hidden platform (p <0.05). On the remaining 3–5 days of the reversal, fbΔBMPRII mutants showed a tendency toward longer escape latencies to find the hidden platform, but once again they were not significantly different than controls (Fig 5A).


Forebrain-Specific Loss of BMPRII in Mice Reduces Anxiety and Increases Object Exploration.

McBrayer ZL, Dimova J, Pisansky MT, Sun M, Beppu H, Gewirtz JC, O'Connor MB - PLoS ONE (2015)

Spatial Learning and Memory in the MWM is Not Affected by Loss of BMPRII.(A) fbΔBMPRII mutant mice (red) do not have a significantly different learning curve than control littermates (black) during the cued (1) or first hidden (2–10) phases of the water maze, but show a slower learning curve during the reversal (12–16) phase of the water maze. (B) The swimming distance of the mice was not different than control littermates, (C) but the swimming speed of the mice was significantly slower on most days of the water maze. The appearance of a slower learning trend during the first hidden phase and reversal is likely an artifact of slow swimming speed rather than slower learning. (D-E) During probe trials fbΔBMPRII mutant mice show similar preference for the target quadrant as control littermates during both the first hidden phase and the reversal phase. Abbreviations: (P) platform position, (Cu) former cued platform position, (F) former hidden platform position. Results reported as mean ± S.E.M. Asterisk indicates *p<0.05, **p<0.005, ***p<0.0005, ****p<0.00005.
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pone.0139860.g005: Spatial Learning and Memory in the MWM is Not Affected by Loss of BMPRII.(A) fbΔBMPRII mutant mice (red) do not have a significantly different learning curve than control littermates (black) during the cued (1) or first hidden (2–10) phases of the water maze, but show a slower learning curve during the reversal (12–16) phase of the water maze. (B) The swimming distance of the mice was not different than control littermates, (C) but the swimming speed of the mice was significantly slower on most days of the water maze. The appearance of a slower learning trend during the first hidden phase and reversal is likely an artifact of slow swimming speed rather than slower learning. (D-E) During probe trials fbΔBMPRII mutant mice show similar preference for the target quadrant as control littermates during both the first hidden phase and the reversal phase. Abbreviations: (P) platform position, (Cu) former cued platform position, (F) former hidden platform position. Results reported as mean ± S.E.M. Asterisk indicates *p<0.05, **p<0.005, ***p<0.0005, ****p<0.00005.
Mentions: During the cued phase of the MWM on day 1, both fbΔBMPRII mutants and control littermates showed similar escape latencies. On days 2–10 of the first hidden platform phase, fbΔBMPRII mutants showed a tendency for slower escape latencies compared to control littermates, but there was no significant effect of genotype (F(1,30) = 3.044, p = 0.0913). During the reversal phase of the hidden platform, fbΔBMPRII mutants showed a slower escape latency that was much more pronounced than during the first hidden phase. A repeated measures two-way ANOVA revealed a significant effect of genotype (F(1,30) = 6.183, p = 0.0187). On the first day of the reversal, mutants and controls showed similar escape latencies, but on the second day of the reversal, fbΔBMPRII mutants took significantly longer to locate the hidden platform (p <0.05). On the remaining 3–5 days of the reversal, fbΔBMPRII mutants showed a tendency toward longer escape latencies to find the hidden platform, but once again they were not significantly different than controls (Fig 5A).

Bottom Line: We found that BMPRII mutant mice had normal spatial learning and memory in the Morris water maze, but showed significantly reduced swimming speeds with increased floating behavior.In the Elevated Plus Maze, BMPRII mutants and Smad4 mutants showed reduced anxiety, while in exploratory tests, BMPRII mutants showed more interest in object exploration.These results suggest that loss of BMPRII in the mouse hippocampus and forebrain does not disrupt spatial learning and memory encoding, but instead impacts exploratory and anxiety-related behaviors.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America.

ABSTRACT
To investigate the role of Bone Morphogenic Protein Receptor Type II (BMPRII) in learning, memory, and exploratory behavior in mice, a tissue-specific knockout of BMPRII in the post-natal hippocampus and forebrain was generated. We found that BMPRII mutant mice had normal spatial learning and memory in the Morris water maze, but showed significantly reduced swimming speeds with increased floating behavior. Further analysis using the Porsolt Swim Test to investigate behavioral despair did not reveal any differences in immobility between mutants and controls. In the Elevated Plus Maze, BMPRII mutants and Smad4 mutants showed reduced anxiety, while in exploratory tests, BMPRII mutants showed more interest in object exploration. These results suggest that loss of BMPRII in the mouse hippocampus and forebrain does not disrupt spatial learning and memory encoding, but instead impacts exploratory and anxiety-related behaviors.

No MeSH data available.


Related in: MedlinePlus