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Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis.

Wu Q, Su N, Chen Q, Shen W, Shen Z, Xia Y, Cui J - PLoS ONE (2015)

Bottom Line: Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment.Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots.Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.

ABSTRACT
Hydrogen gas (H2) was recently proposed as a therapeutic antioxidant and signaling molecule in clinical trials. However, the underlying physiological roles of H2 in plants remain unclear. In the present study, hydrogen-rich water (HRW) was used to characterize the physiological roles of H2 in enhancing the tolerance of Brassica campestris against cadmium (Cd). The results showed that both 50 μM CdCl2 and 50%-saturated HRW induced an increase of endogenous H2 in Brassica campestris seedlings, and HRW alleviated Cd toxicity related to growth inhibition and oxidative damage. Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment. Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots. Molecular evidence illustrated that the expression of genes such as GS, GR1 and GR2, which were down-regulated following the addition of Cd, GSH or BSO, could be reversed to varying degrees by the addition of HRW. Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.

No MeSH data available.


Related in: MedlinePlus

Schematic representation of the pathway induced by cadmium including the participation of H2, GSH and AsA.
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pone.0139956.g010: Schematic representation of the pathway induced by cadmium including the participation of H2, GSH and AsA.

Mentions: Taken together, we proposed that H2 was able to effectively alleviate Cd stress by exogenously applied HRW, and GSH played an important role in this process. Previous researches reported that γ-GCS and GR led to the enhanced GSH production and the larger total GSH pool size [65]. Furthermore, glutathione showed a strong antioxidant effect against different environment stresses mainly through GSH-dependent regeneration of ascorbate, GSH-dependent peroxide metabolism and GSH itself [66, 67]. In this report, when plants were damaged by Cd, more H2 production was induced, followed by promoted γ-GCS and GR activity. This increase of enzyme activities promoted GSH synthesis and reduction from GSSG, respectively. This rise in concentration of GSH was beneficial to the utilization of DHA reductase (DHAR) which thereby reduced DHA to AsA. In addition to the AsA-GSH cycle, other proteins also used GSH as substrate like GPX and GST, and their activities were also strengthened. All these results indicate that H2-induced glutathione homeostasis reestablishment exerts a major role in alleviation of Cd stress through ROS detoxification (Fig 10).


Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis.

Wu Q, Su N, Chen Q, Shen W, Shen Z, Xia Y, Cui J - PLoS ONE (2015)

Schematic representation of the pathway induced by cadmium including the participation of H2, GSH and AsA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4596834&req=5

pone.0139956.g010: Schematic representation of the pathway induced by cadmium including the participation of H2, GSH and AsA.
Mentions: Taken together, we proposed that H2 was able to effectively alleviate Cd stress by exogenously applied HRW, and GSH played an important role in this process. Previous researches reported that γ-GCS and GR led to the enhanced GSH production and the larger total GSH pool size [65]. Furthermore, glutathione showed a strong antioxidant effect against different environment stresses mainly through GSH-dependent regeneration of ascorbate, GSH-dependent peroxide metabolism and GSH itself [66, 67]. In this report, when plants were damaged by Cd, more H2 production was induced, followed by promoted γ-GCS and GR activity. This increase of enzyme activities promoted GSH synthesis and reduction from GSSG, respectively. This rise in concentration of GSH was beneficial to the utilization of DHA reductase (DHAR) which thereby reduced DHA to AsA. In addition to the AsA-GSH cycle, other proteins also used GSH as substrate like GPX and GST, and their activities were also strengthened. All these results indicate that H2-induced glutathione homeostasis reestablishment exerts a major role in alleviation of Cd stress through ROS detoxification (Fig 10).

Bottom Line: Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment.Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots.Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.

ABSTRACT
Hydrogen gas (H2) was recently proposed as a therapeutic antioxidant and signaling molecule in clinical trials. However, the underlying physiological roles of H2 in plants remain unclear. In the present study, hydrogen-rich water (HRW) was used to characterize the physiological roles of H2 in enhancing the tolerance of Brassica campestris against cadmium (Cd). The results showed that both 50 μM CdCl2 and 50%-saturated HRW induced an increase of endogenous H2 in Brassica campestris seedlings, and HRW alleviated Cd toxicity related to growth inhibition and oxidative damage. Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment. Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots. Molecular evidence illustrated that the expression of genes such as GS, GR1 and GR2, which were down-regulated following the addition of Cd, GSH or BSO, could be reversed to varying degrees by the addition of HRW. Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.

No MeSH data available.


Related in: MedlinePlus