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Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis.

Wu Q, Su N, Chen Q, Shen W, Shen Z, Xia Y, Cui J - PLoS ONE (2015)

Bottom Line: Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment.Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots.Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.

ABSTRACT
Hydrogen gas (H2) was recently proposed as a therapeutic antioxidant and signaling molecule in clinical trials. However, the underlying physiological roles of H2 in plants remain unclear. In the present study, hydrogen-rich water (HRW) was used to characterize the physiological roles of H2 in enhancing the tolerance of Brassica campestris against cadmium (Cd). The results showed that both 50 μM CdCl2 and 50%-saturated HRW induced an increase of endogenous H2 in Brassica campestris seedlings, and HRW alleviated Cd toxicity related to growth inhibition and oxidative damage. Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment. Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots. Molecular evidence illustrated that the expression of genes such as GS, GR1 and GR2, which were down-regulated following the addition of Cd, GSH or BSO, could be reversed to varying degrees by the addition of HRW. Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.

No MeSH data available.


Related in: MedlinePlus

Effects of GSH, BSO and HRW alone or their combinations on gene expression of GCS, GS, GR1, GR2, GPX and GST in the roots of Brassica campestris upon Cd or none stress.Expression levels of corresponding genes are presented relative to the control samples, with normalized against expression of two internal reference genes in each sample. Data are means ± SE from three independent experiments. Bars with different letters are significantly different at P<0.05 according to Duncan’s multiple range test.
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pone.0139956.g008: Effects of GSH, BSO and HRW alone or their combinations on gene expression of GCS, GS, GR1, GR2, GPX and GST in the roots of Brassica campestris upon Cd or none stress.Expression levels of corresponding genes are presented relative to the control samples, with normalized against expression of two internal reference genes in each sample. Data are means ± SE from three independent experiments. Bars with different letters are significantly different at P<0.05 according to Duncan’s multiple range test.

Mentions: Subsequent gene analysis revealed that seedlings treated with HRW substantially enhanced the transcription levels of genes such as GCS, GS, GR1 and GR2 in the non-Cd pretreatment. A similar HRW-triggered induction of the expression of GCS, GS, GR1, GR2 and GST was also observed in the Cd→HRW treatment compared to the Cd→H2O treatment. In treatments with GSH or BSO, the expression of GCS, GS, GR1 and GR2 declined significantly compared with the Cd-free control samples, whereas co-treatment with HRW blocked this decrease to varying degrees (Fig 8A and 8D). The transcript levels of the GPX and GST genes were increased by pre-treatment with Cd, an effect that was blocked by the addition of GSH or BSO. However, this block was partially reversed by co-treatment with HRW (Fig 8E and 8F).


Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis.

Wu Q, Su N, Chen Q, Shen W, Shen Z, Xia Y, Cui J - PLoS ONE (2015)

Effects of GSH, BSO and HRW alone or their combinations on gene expression of GCS, GS, GR1, GR2, GPX and GST in the roots of Brassica campestris upon Cd or none stress.Expression levels of corresponding genes are presented relative to the control samples, with normalized against expression of two internal reference genes in each sample. Data are means ± SE from three independent experiments. Bars with different letters are significantly different at P<0.05 according to Duncan’s multiple range test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4596834&req=5

pone.0139956.g008: Effects of GSH, BSO and HRW alone or their combinations on gene expression of GCS, GS, GR1, GR2, GPX and GST in the roots of Brassica campestris upon Cd or none stress.Expression levels of corresponding genes are presented relative to the control samples, with normalized against expression of two internal reference genes in each sample. Data are means ± SE from three independent experiments. Bars with different letters are significantly different at P<0.05 according to Duncan’s multiple range test.
Mentions: Subsequent gene analysis revealed that seedlings treated with HRW substantially enhanced the transcription levels of genes such as GCS, GS, GR1 and GR2 in the non-Cd pretreatment. A similar HRW-triggered induction of the expression of GCS, GS, GR1, GR2 and GST was also observed in the Cd→HRW treatment compared to the Cd→H2O treatment. In treatments with GSH or BSO, the expression of GCS, GS, GR1 and GR2 declined significantly compared with the Cd-free control samples, whereas co-treatment with HRW blocked this decrease to varying degrees (Fig 8A and 8D). The transcript levels of the GPX and GST genes were increased by pre-treatment with Cd, an effect that was blocked by the addition of GSH or BSO. However, this block was partially reversed by co-treatment with HRW (Fig 8E and 8F).

Bottom Line: Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment.Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots.Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.

ABSTRACT
Hydrogen gas (H2) was recently proposed as a therapeutic antioxidant and signaling molecule in clinical trials. However, the underlying physiological roles of H2 in plants remain unclear. In the present study, hydrogen-rich water (HRW) was used to characterize the physiological roles of H2 in enhancing the tolerance of Brassica campestris against cadmium (Cd). The results showed that both 50 μM CdCl2 and 50%-saturated HRW induced an increase of endogenous H2 in Brassica campestris seedlings, and HRW alleviated Cd toxicity related to growth inhibition and oxidative damage. Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment. Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots. Molecular evidence illustrated that the expression of genes such as GS, GR1 and GR2, which were down-regulated following the addition of Cd, GSH or BSO, could be reversed to varying degrees by the addition of HRW. Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.

No MeSH data available.


Related in: MedlinePlus