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Conditional Inhibition of Adult Neurogenesis by Inducible and Targeted Deletion of ERK5 MAP Kinase Is Not Associated with Anxiety/Depression-Like Behaviors(1,2).

Zou J, Wang W, Pan YW, Abel GM, Storm DR, Xia Z - eNeuro (2015)

Bottom Line: Although there is evidence that adult neurogenesis contributes to the therapeutic efficacy of chronic antidepressant treatment for anxiety and depression disorders, the role of adult neurogenesis in the onset of depression-related symptoms is still open to question.To address this issue, we utilized a transgenic mouse strain in which adult neurogenesis was specifically and conditionally impaired by Nestin-CreER-driven, inducible knockout (icKO) of erk5 MAP kinase in Nestin-expressing neural progenitors of the adult mouse brain upon tamoxifen administration.Our findings indicate that impaired adult neurogenesis does not lead to anxiety or depression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Toxicology Program in the Department of Environmental and Occupational Health Sciences.

ABSTRACT
Although there is evidence that adult neurogenesis contributes to the therapeutic efficacy of chronic antidepressant treatment for anxiety and depression disorders, the role of adult neurogenesis in the onset of depression-related symptoms is still open to question. To address this issue, we utilized a transgenic mouse strain in which adult neurogenesis was specifically and conditionally impaired by Nestin-CreER-driven, inducible knockout (icKO) of erk5 MAP kinase in Nestin-expressing neural progenitors of the adult mouse brain upon tamoxifen administration. Here, we report that inhibition of adult neurogenesis by this mechanism is not associated with an increase of the baseline anxiety or depression in non-stressed animals, nor does it increase the animal's susceptibility to depression after chronic unpredictable stress treatment. Our findings indicate that impaired adult neurogenesis does not lead to anxiety or depression.

No MeSH data available.


Related in: MedlinePlus

ERK5 icKO mice do not display reduced grooming behavior in the splash test. Mice were splashed with 10% sucrose solution on the abdomen and their grooming behavior was observed in the home cage for 5 min. A, Latency to first groom. B, Total time spent grooming. C, Number of grooming episodes. n.s., Not significant.
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Figure 9: ERK5 icKO mice do not display reduced grooming behavior in the splash test. Mice were splashed with 10% sucrose solution on the abdomen and their grooming behavior was observed in the home cage for 5 min. A, Latency to first groom. B, Total time spent grooming. C, Number of grooming episodes. n.s., Not significant.

Mentions: To assess whether the inhibition of adult neurogenesis by ERK5 deletion is associated with depression-like behavior, we first evaluated self-care, specifically the grooming behavior, of the ERK5 icKO mice and control mice using the splash test. Mice were splashed with sucrose solution on their abdomen and their grooming behavior was then scored. We did not observe a difference between the two groups of mice in their latency to groom (p = 0.205)17, grooming frequency (p = 0.899)18, or the duration of grooming (p = 0.415)19 (Fig. 9), suggesting that inhibition of adult neurogenesis by ERK5 deletion does not affect self-care in non-stressed animals.


Conditional Inhibition of Adult Neurogenesis by Inducible and Targeted Deletion of ERK5 MAP Kinase Is Not Associated with Anxiety/Depression-Like Behaviors(1,2).

Zou J, Wang W, Pan YW, Abel GM, Storm DR, Xia Z - eNeuro (2015)

ERK5 icKO mice do not display reduced grooming behavior in the splash test. Mice were splashed with 10% sucrose solution on the abdomen and their grooming behavior was observed in the home cage for 5 min. A, Latency to first groom. B, Total time spent grooming. C, Number of grooming episodes. n.s., Not significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4596085&req=5

Figure 9: ERK5 icKO mice do not display reduced grooming behavior in the splash test. Mice were splashed with 10% sucrose solution on the abdomen and their grooming behavior was observed in the home cage for 5 min. A, Latency to first groom. B, Total time spent grooming. C, Number of grooming episodes. n.s., Not significant.
Mentions: To assess whether the inhibition of adult neurogenesis by ERK5 deletion is associated with depression-like behavior, we first evaluated self-care, specifically the grooming behavior, of the ERK5 icKO mice and control mice using the splash test. Mice were splashed with sucrose solution on their abdomen and their grooming behavior was then scored. We did not observe a difference between the two groups of mice in their latency to groom (p = 0.205)17, grooming frequency (p = 0.899)18, or the duration of grooming (p = 0.415)19 (Fig. 9), suggesting that inhibition of adult neurogenesis by ERK5 deletion does not affect self-care in non-stressed animals.

Bottom Line: Although there is evidence that adult neurogenesis contributes to the therapeutic efficacy of chronic antidepressant treatment for anxiety and depression disorders, the role of adult neurogenesis in the onset of depression-related symptoms is still open to question.To address this issue, we utilized a transgenic mouse strain in which adult neurogenesis was specifically and conditionally impaired by Nestin-CreER-driven, inducible knockout (icKO) of erk5 MAP kinase in Nestin-expressing neural progenitors of the adult mouse brain upon tamoxifen administration.Our findings indicate that impaired adult neurogenesis does not lead to anxiety or depression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Toxicology Program in the Department of Environmental and Occupational Health Sciences.

ABSTRACT
Although there is evidence that adult neurogenesis contributes to the therapeutic efficacy of chronic antidepressant treatment for anxiety and depression disorders, the role of adult neurogenesis in the onset of depression-related symptoms is still open to question. To address this issue, we utilized a transgenic mouse strain in which adult neurogenesis was specifically and conditionally impaired by Nestin-CreER-driven, inducible knockout (icKO) of erk5 MAP kinase in Nestin-expressing neural progenitors of the adult mouse brain upon tamoxifen administration. Here, we report that inhibition of adult neurogenesis by this mechanism is not associated with an increase of the baseline anxiety or depression in non-stressed animals, nor does it increase the animal's susceptibility to depression after chronic unpredictable stress treatment. Our findings indicate that impaired adult neurogenesis does not lead to anxiety or depression.

No MeSH data available.


Related in: MedlinePlus