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Conditional Inhibition of Adult Neurogenesis by Inducible and Targeted Deletion of ERK5 MAP Kinase Is Not Associated with Anxiety/Depression-Like Behaviors(1,2).

Zou J, Wang W, Pan YW, Abel GM, Storm DR, Xia Z - eNeuro (2015)

Bottom Line: Although there is evidence that adult neurogenesis contributes to the therapeutic efficacy of chronic antidepressant treatment for anxiety and depression disorders, the role of adult neurogenesis in the onset of depression-related symptoms is still open to question.To address this issue, we utilized a transgenic mouse strain in which adult neurogenesis was specifically and conditionally impaired by Nestin-CreER-driven, inducible knockout (icKO) of erk5 MAP kinase in Nestin-expressing neural progenitors of the adult mouse brain upon tamoxifen administration.Our findings indicate that impaired adult neurogenesis does not lead to anxiety or depression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Toxicology Program in the Department of Environmental and Occupational Health Sciences.

ABSTRACT
Although there is evidence that adult neurogenesis contributes to the therapeutic efficacy of chronic antidepressant treatment for anxiety and depression disorders, the role of adult neurogenesis in the onset of depression-related symptoms is still open to question. To address this issue, we utilized a transgenic mouse strain in which adult neurogenesis was specifically and conditionally impaired by Nestin-CreER-driven, inducible knockout (icKO) of erk5 MAP kinase in Nestin-expressing neural progenitors of the adult mouse brain upon tamoxifen administration. Here, we report that inhibition of adult neurogenesis by this mechanism is not associated with an increase of the baseline anxiety or depression in non-stressed animals, nor does it increase the animal's susceptibility to depression after chronic unpredictable stress treatment. Our findings indicate that impaired adult neurogenesis does not lead to anxiety or depression.

No MeSH data available.


Related in: MedlinePlus

ERK5 icKO mice behave the same as control mice in a novelty-induced hypophagia test. Individually housed mice were trained with chocolate milk 30 min per day on 3 consecutive days in their home cages. On the fourth day, each mouse was transferred into a new mouse cage without bedding but with the access to a bottle of chocolate milk. The mouse was allowed to drink the milk freely for 15 min. A, Latency to drink the chocolate milk. B, Total number of trips to the chocolate milk. n.s., Not significant.
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Figure 8: ERK5 icKO mice behave the same as control mice in a novelty-induced hypophagia test. Individually housed mice were trained with chocolate milk 30 min per day on 3 consecutive days in their home cages. On the fourth day, each mouse was transferred into a new mouse cage without bedding but with the access to a bottle of chocolate milk. The mouse was allowed to drink the milk freely for 15 min. A, Latency to drink the chocolate milk. B, Total number of trips to the chocolate milk. n.s., Not significant.

Mentions: Mice were also subjected to another behavior test for hypophagia, the novelty-induced hypophagia test (Dulawa and Hen, 2005), in which a palatable snack (chocolate milk) was used and no food restriction is required. The test was performed in a new mouse cage without bedding, an unfamiliar but less aversive environment. Again, there was no difference between ERK5 icKO mice and control mice in the latency to drink the chocolate milk (p = 0.935)15 or the frequency of drinking (p = 0.387)16 within the observation period, confirming that ERK5 deletion does not cause hypophagia (Fig. 8).


Conditional Inhibition of Adult Neurogenesis by Inducible and Targeted Deletion of ERK5 MAP Kinase Is Not Associated with Anxiety/Depression-Like Behaviors(1,2).

Zou J, Wang W, Pan YW, Abel GM, Storm DR, Xia Z - eNeuro (2015)

ERK5 icKO mice behave the same as control mice in a novelty-induced hypophagia test. Individually housed mice were trained with chocolate milk 30 min per day on 3 consecutive days in their home cages. On the fourth day, each mouse was transferred into a new mouse cage without bedding but with the access to a bottle of chocolate milk. The mouse was allowed to drink the milk freely for 15 min. A, Latency to drink the chocolate milk. B, Total number of trips to the chocolate milk. n.s., Not significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4596085&req=5

Figure 8: ERK5 icKO mice behave the same as control mice in a novelty-induced hypophagia test. Individually housed mice were trained with chocolate milk 30 min per day on 3 consecutive days in their home cages. On the fourth day, each mouse was transferred into a new mouse cage without bedding but with the access to a bottle of chocolate milk. The mouse was allowed to drink the milk freely for 15 min. A, Latency to drink the chocolate milk. B, Total number of trips to the chocolate milk. n.s., Not significant.
Mentions: Mice were also subjected to another behavior test for hypophagia, the novelty-induced hypophagia test (Dulawa and Hen, 2005), in which a palatable snack (chocolate milk) was used and no food restriction is required. The test was performed in a new mouse cage without bedding, an unfamiliar but less aversive environment. Again, there was no difference between ERK5 icKO mice and control mice in the latency to drink the chocolate milk (p = 0.935)15 or the frequency of drinking (p = 0.387)16 within the observation period, confirming that ERK5 deletion does not cause hypophagia (Fig. 8).

Bottom Line: Although there is evidence that adult neurogenesis contributes to the therapeutic efficacy of chronic antidepressant treatment for anxiety and depression disorders, the role of adult neurogenesis in the onset of depression-related symptoms is still open to question.To address this issue, we utilized a transgenic mouse strain in which adult neurogenesis was specifically and conditionally impaired by Nestin-CreER-driven, inducible knockout (icKO) of erk5 MAP kinase in Nestin-expressing neural progenitors of the adult mouse brain upon tamoxifen administration.Our findings indicate that impaired adult neurogenesis does not lead to anxiety or depression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Toxicology Program in the Department of Environmental and Occupational Health Sciences.

ABSTRACT
Although there is evidence that adult neurogenesis contributes to the therapeutic efficacy of chronic antidepressant treatment for anxiety and depression disorders, the role of adult neurogenesis in the onset of depression-related symptoms is still open to question. To address this issue, we utilized a transgenic mouse strain in which adult neurogenesis was specifically and conditionally impaired by Nestin-CreER-driven, inducible knockout (icKO) of erk5 MAP kinase in Nestin-expressing neural progenitors of the adult mouse brain upon tamoxifen administration. Here, we report that inhibition of adult neurogenesis by this mechanism is not associated with an increase of the baseline anxiety or depression in non-stressed animals, nor does it increase the animal's susceptibility to depression after chronic unpredictable stress treatment. Our findings indicate that impaired adult neurogenesis does not lead to anxiety or depression.

No MeSH data available.


Related in: MedlinePlus