Limits...
Dendritic morphology, synaptic transmission, and activity of mature granule cells born following pilocarpine-induced status epilepticus in the rat.

Gao F, Song X, Zhu D, Wang X, Hao A, Nadler JV, Zhan RZ - Front Cell Neurosci (2015)

Bottom Line: The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling.After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells.Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Shandong University School of Medicine Jinan, China.

ABSTRACT
To understand the potential role of enhanced hippocampal neurogenesis after pilocarpine-induced status epilepticus (SE) in the development of epilepsy, we quantitatively analyzed the geometry of apical dendrites, synaptic transmission, and activation levels of normotopically distributed mature newborn granule cells in the rat. SE in male Sprague-Dawley rats (between 6 and 7 weeks old) lasting for more than 2 h was induced by an intraperitoneal injection of pilocarpine. The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling. Mature granule cells born after SE had dendritic complexity similar to that of granule cells born naturally, but with denser mushroom-like spines in dendritic segments located in the outer molecular layer. Miniature inhibitory post-synaptic currents (mIPSCs) were similar between the controls and rats subjected to SE; however, smaller miniature excitatory post-synaptic current (mEPSC) amplitude with a trend toward less frequent was found in mature granule cells born after SE. After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells. Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

No MeSH data available.


Related in: MedlinePlus

Sholl analysis dedicates the dendritic complexity of mature newborn granule cells in control and SE rats. Z-series stacks of 2 μm thick were taken in GFP-positive cells located in the suprapyramidal blade with a 20X objective (zoom = 1) and, thereafter, a 3D-image was created for each cell. After the soma and the apical dendritic arborizations were traced under ImageJ (http://imagej.nih.gov/ij/) with the NeuronJ plugin (http://www.imagescience.org/meijering/software/neuronj//), Sholl analysis was carried out by using ImageJ with the “Sholl analysis” plugin. (A) Representative images of retroviral labeling of newborn granule cells in a control (left panel) and an SE rat (right panel). Note that the granule cell born after SE has branch orders and arborizations similar to that of one born naturally. (B) Bar graph shows the dendritic complexity of newborn granule cells revealed by Sholl analysis. In both the control and SE groups (n = 8 animals each), 3–4 cells for each rat were scanned and all values at a given distance were averaged. Data are statistically compared using repeated-measure ANOVA.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4596052&req=5

Figure 2: Sholl analysis dedicates the dendritic complexity of mature newborn granule cells in control and SE rats. Z-series stacks of 2 μm thick were taken in GFP-positive cells located in the suprapyramidal blade with a 20X objective (zoom = 1) and, thereafter, a 3D-image was created for each cell. After the soma and the apical dendritic arborizations were traced under ImageJ (http://imagej.nih.gov/ij/) with the NeuronJ plugin (http://www.imagescience.org/meijering/software/neuronj//), Sholl analysis was carried out by using ImageJ with the “Sholl analysis” plugin. (A) Representative images of retroviral labeling of newborn granule cells in a control (left panel) and an SE rat (right panel). Note that the granule cell born after SE has branch orders and arborizations similar to that of one born naturally. (B) Bar graph shows the dendritic complexity of newborn granule cells revealed by Sholl analysis. In both the control and SE groups (n = 8 animals each), 3–4 cells for each rat were scanned and all values at a given distance were averaged. Data are statistically compared using repeated-measure ANOVA.

Mentions: Sholl analysis was used to determine the branching of apical dendrites. As shown in the left panel of Figure 2A, the apical dendrite of granule cells born in a control rat had 4–5 branch orders and their distal branches always reached the outer molecular layer. The apical dendrites of granule cells born after SE had very similar dendritic branch orders and arborizations also extended into the outer molecular layer (Figure 2A, right panel). Quantitative data were collected from eight rats in either control or SE group (Figure 2B); for each rat, 3–4 cells were scanned and the values are averaged to present the animal. Statistical comparisons done with repeated measure ANOVA revealed no statistical difference [F(0.01, 1), p = 0.942] in dendritic branching between mature granule cells born after SE and those granule cells born naturally.


Dendritic morphology, synaptic transmission, and activity of mature granule cells born following pilocarpine-induced status epilepticus in the rat.

Gao F, Song X, Zhu D, Wang X, Hao A, Nadler JV, Zhan RZ - Front Cell Neurosci (2015)

Sholl analysis dedicates the dendritic complexity of mature newborn granule cells in control and SE rats. Z-series stacks of 2 μm thick were taken in GFP-positive cells located in the suprapyramidal blade with a 20X objective (zoom = 1) and, thereafter, a 3D-image was created for each cell. After the soma and the apical dendritic arborizations were traced under ImageJ (http://imagej.nih.gov/ij/) with the NeuronJ plugin (http://www.imagescience.org/meijering/software/neuronj//), Sholl analysis was carried out by using ImageJ with the “Sholl analysis” plugin. (A) Representative images of retroviral labeling of newborn granule cells in a control (left panel) and an SE rat (right panel). Note that the granule cell born after SE has branch orders and arborizations similar to that of one born naturally. (B) Bar graph shows the dendritic complexity of newborn granule cells revealed by Sholl analysis. In both the control and SE groups (n = 8 animals each), 3–4 cells for each rat were scanned and all values at a given distance were averaged. Data are statistically compared using repeated-measure ANOVA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4596052&req=5

Figure 2: Sholl analysis dedicates the dendritic complexity of mature newborn granule cells in control and SE rats. Z-series stacks of 2 μm thick were taken in GFP-positive cells located in the suprapyramidal blade with a 20X objective (zoom = 1) and, thereafter, a 3D-image was created for each cell. After the soma and the apical dendritic arborizations were traced under ImageJ (http://imagej.nih.gov/ij/) with the NeuronJ plugin (http://www.imagescience.org/meijering/software/neuronj//), Sholl analysis was carried out by using ImageJ with the “Sholl analysis” plugin. (A) Representative images of retroviral labeling of newborn granule cells in a control (left panel) and an SE rat (right panel). Note that the granule cell born after SE has branch orders and arborizations similar to that of one born naturally. (B) Bar graph shows the dendritic complexity of newborn granule cells revealed by Sholl analysis. In both the control and SE groups (n = 8 animals each), 3–4 cells for each rat were scanned and all values at a given distance were averaged. Data are statistically compared using repeated-measure ANOVA.
Mentions: Sholl analysis was used to determine the branching of apical dendrites. As shown in the left panel of Figure 2A, the apical dendrite of granule cells born in a control rat had 4–5 branch orders and their distal branches always reached the outer molecular layer. The apical dendrites of granule cells born after SE had very similar dendritic branch orders and arborizations also extended into the outer molecular layer (Figure 2A, right panel). Quantitative data were collected from eight rats in either control or SE group (Figure 2B); for each rat, 3–4 cells were scanned and the values are averaged to present the animal. Statistical comparisons done with repeated measure ANOVA revealed no statistical difference [F(0.01, 1), p = 0.942] in dendritic branching between mature granule cells born after SE and those granule cells born naturally.

Bottom Line: The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling.After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells.Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Shandong University School of Medicine Jinan, China.

ABSTRACT
To understand the potential role of enhanced hippocampal neurogenesis after pilocarpine-induced status epilepticus (SE) in the development of epilepsy, we quantitatively analyzed the geometry of apical dendrites, synaptic transmission, and activation levels of normotopically distributed mature newborn granule cells in the rat. SE in male Sprague-Dawley rats (between 6 and 7 weeks old) lasting for more than 2 h was induced by an intraperitoneal injection of pilocarpine. The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling. Mature granule cells born after SE had dendritic complexity similar to that of granule cells born naturally, but with denser mushroom-like spines in dendritic segments located in the outer molecular layer. Miniature inhibitory post-synaptic currents (mIPSCs) were similar between the controls and rats subjected to SE; however, smaller miniature excitatory post-synaptic current (mEPSC) amplitude with a trend toward less frequent was found in mature granule cells born after SE. After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells. Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

No MeSH data available.


Related in: MedlinePlus