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Dendritic morphology, synaptic transmission, and activity of mature granule cells born following pilocarpine-induced status epilepticus in the rat.

Gao F, Song X, Zhu D, Wang X, Hao A, Nadler JV, Zhan RZ - Front Cell Neurosci (2015)

Bottom Line: The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling.After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells.Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Shandong University School of Medicine Jinan, China.

ABSTRACT
To understand the potential role of enhanced hippocampal neurogenesis after pilocarpine-induced status epilepticus (SE) in the development of epilepsy, we quantitatively analyzed the geometry of apical dendrites, synaptic transmission, and activation levels of normotopically distributed mature newborn granule cells in the rat. SE in male Sprague-Dawley rats (between 6 and 7 weeks old) lasting for more than 2 h was induced by an intraperitoneal injection of pilocarpine. The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling. Mature granule cells born after SE had dendritic complexity similar to that of granule cells born naturally, but with denser mushroom-like spines in dendritic segments located in the outer molecular layer. Miniature inhibitory post-synaptic currents (mIPSCs) were similar between the controls and rats subjected to SE; however, smaller miniature excitatory post-synaptic current (mEPSC) amplitude with a trend toward less frequent was found in mature granule cells born after SE. After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells. Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

No MeSH data available.


Related in: MedlinePlus

Representative images show 4-month old newborn granule cells labeled by the CAG-GFP retroviral vector in the control and SE rats. Rats were sacrificed 4 months after the CAG-GFP retroviral vector injection and coronal sections through the right hippocampus were cut into 80 μm. Z-series stacks of 2 μm were taken by a Zeiss LSM 780 confocal microscope. The white box-indicated regions in the left panels were scanned under a higher objective and are shown in the right panels, correspondingly. Arrows indicate the newborn granule cell that has a basal dendrite attached to the soma. ML, molecular layer; GCL, granule cell layer.
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Figure 1: Representative images show 4-month old newborn granule cells labeled by the CAG-GFP retroviral vector in the control and SE rats. Rats were sacrificed 4 months after the CAG-GFP retroviral vector injection and coronal sections through the right hippocampus were cut into 80 μm. Z-series stacks of 2 μm were taken by a Zeiss LSM 780 confocal microscope. The white box-indicated regions in the left panels were scanned under a higher objective and are shown in the right panels, correspondingly. Arrows indicate the newborn granule cell that has a basal dendrite attached to the soma. ML, molecular layer; GCL, granule cell layer.

Mentions: One microliter of retroviral CAG-GFP vector injected into the dentate gyrus labeled granule cells for approximately 3 mm in the septotemporal direction. More than 10 weeks after viral vector injection, GFP-expressing somata with processes could be seen in both the suprapyramidal and infrapyramidal blades of control or SE rats. These cells were scattered along the granule cell layer-hilus border (Figure 1). Occasionally, cells with a single basal dendrite that extended into the hilus were noticed; more often they were seen in SE rats.


Dendritic morphology, synaptic transmission, and activity of mature granule cells born following pilocarpine-induced status epilepticus in the rat.

Gao F, Song X, Zhu D, Wang X, Hao A, Nadler JV, Zhan RZ - Front Cell Neurosci (2015)

Representative images show 4-month old newborn granule cells labeled by the CAG-GFP retroviral vector in the control and SE rats. Rats were sacrificed 4 months after the CAG-GFP retroviral vector injection and coronal sections through the right hippocampus were cut into 80 μm. Z-series stacks of 2 μm were taken by a Zeiss LSM 780 confocal microscope. The white box-indicated regions in the left panels were scanned under a higher objective and are shown in the right panels, correspondingly. Arrows indicate the newborn granule cell that has a basal dendrite attached to the soma. ML, molecular layer; GCL, granule cell layer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4596052&req=5

Figure 1: Representative images show 4-month old newborn granule cells labeled by the CAG-GFP retroviral vector in the control and SE rats. Rats were sacrificed 4 months after the CAG-GFP retroviral vector injection and coronal sections through the right hippocampus were cut into 80 μm. Z-series stacks of 2 μm were taken by a Zeiss LSM 780 confocal microscope. The white box-indicated regions in the left panels were scanned under a higher objective and are shown in the right panels, correspondingly. Arrows indicate the newborn granule cell that has a basal dendrite attached to the soma. ML, molecular layer; GCL, granule cell layer.
Mentions: One microliter of retroviral CAG-GFP vector injected into the dentate gyrus labeled granule cells for approximately 3 mm in the septotemporal direction. More than 10 weeks after viral vector injection, GFP-expressing somata with processes could be seen in both the suprapyramidal and infrapyramidal blades of control or SE rats. These cells were scattered along the granule cell layer-hilus border (Figure 1). Occasionally, cells with a single basal dendrite that extended into the hilus were noticed; more often they were seen in SE rats.

Bottom Line: The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling.After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells.Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Shandong University School of Medicine Jinan, China.

ABSTRACT
To understand the potential role of enhanced hippocampal neurogenesis after pilocarpine-induced status epilepticus (SE) in the development of epilepsy, we quantitatively analyzed the geometry of apical dendrites, synaptic transmission, and activation levels of normotopically distributed mature newborn granule cells in the rat. SE in male Sprague-Dawley rats (between 6 and 7 weeks old) lasting for more than 2 h was induced by an intraperitoneal injection of pilocarpine. The complexity, spine density, miniature post-synaptic currents, and activity-regulated cytoskeleton-associated protein (Arc) expression of granule cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP retroviral vector-mediated labeling. Mature granule cells born after SE had dendritic complexity similar to that of granule cells born naturally, but with denser mushroom-like spines in dendritic segments located in the outer molecular layer. Miniature inhibitory post-synaptic currents (mIPSCs) were similar between the controls and rats subjected to SE; however, smaller miniature excitatory post-synaptic current (mEPSC) amplitude with a trend toward less frequent was found in mature granule cells born after SE. After maturation, granule cells born after SE did not show denser Arc expression in the resting condition or 2 h after being activated by pentylenetetrazol-induced transient seizure activity than vicinal GFP-unlabeled granule cells. Thus our results suggest that normotopic granule cells born after pilocarpine-induced SE are no more active when mature than age-matched, naturally born granule cells.

No MeSH data available.


Related in: MedlinePlus