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Relationship between gene expression and the accumulation of catechin during spring and autumn in tea plants (Camellia sinensis L.).

Liu M, Tian HL, Wu JH, Cang RR, Wang RX, Qi XH, Xu Q, Chen XH - Hortic Res (2015)

Bottom Line: Tea is popular worldwide given the plant's health benefits.The results indicated that the total catechin (TC) concentrations were significantly higher in tea plants harvested in autumn than in those harvested in spring, based on higher concentrations of epigallocatechin (EGC) in autumn tea (P<0.01).The expression of the genes phenylalanine ammonia-lyase (PAL), flavanone 3-hydroxylase (F3H), flavonoid 3',5'-hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) is closely related to the TC content of tea plants in both spring and autumn.

View Article: PubMed Central - PubMed

Affiliation: School of Horticulture and Plant Protection, Yangzhou University , 48 Wenhui East Road, Yangzhou, Jiangsu 225009, P. R. China.

ABSTRACT
The tea plant (Camellia sinensis L.) is an important commercial crop with remarkably high catechin concentrations. Tea is popular worldwide given the plant's health benefits. Catechins are the main astringent substance in tea and are synthesized mainly via the phenylpropanoid pathway. In this study, eight cultivars of tea plants harvested both in spring and autumn were used to investigate differences in catechin concentrations by using high-performance liquid chromatography. The expression levels of genes associated with catechin biosynthesis were investigated using reverse transcription-quantitative polymerase chain reaction. The results indicated that the total catechin (TC) concentrations were significantly higher in tea plants harvested in autumn than in those harvested in spring, based on higher concentrations of epigallocatechin (EGC) in autumn tea (P<0.01). The expression of the genes phenylalanine ammonia-lyase (PAL), flavanone 3-hydroxylase (F3H), flavonoid 3',5'-hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) is closely related to the TC content of tea plants in both spring and autumn. Positive correlations between PAL, cinnamate 4-hydroxylase (C4H), F3H, and DFR expression and EGC accumulation in autumn tea were identified, with correlation coefficients of 0.710, 0.763, 0.884, and 0.707, respectively. A negative correlation between ANS expression level and EGC concentrations in tea plants harvested in spring was noted (r=-0.732). Additionally, negative correlations between F3H and ANS expression levels and the catechin content were identified in spring tea, whereas the correlations were positive in autumn tea. Significant differences in the F3H and ANS expression levels between spring and autumn tea indicate that F3H and ANS are potentially key genes affecting catechin accumulation in tea plants.

No MeSH data available.


Expression of genes F3H and ANS encoding catechin biosynthesis enzymes in bud leaves of eight varieties of tea plants relative to β-actin gene, used as an internal control. Error bars indicate SD of three technical replicates.
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fig4: Expression of genes F3H and ANS encoding catechin biosynthesis enzymes in bud leaves of eight varieties of tea plants relative to β-actin gene, used as an internal control. Error bars indicate SD of three technical replicates.

Mentions: From Tables 5 and 6, correlations between catechin content and PAL, F3H, F3′5′H, DFR, and ANS gene expression were observed in the bud leaves of eight varieties in spring and autumn. A positive correlation between TC and LAR expression (r=0.707) was observed in spring tea, but a positive correlation was noted between C4H expression and TC levels in autumn tea. F3H and ANS expression were downregulated in autumn tea as compared with spring tea, and catechin accumulation was higher in autumn tea than in spring tea (Figure 4). Catechin concentrations were negatively correlated with F3H and ANS expression levels in spring tea but positively correlated in autumn tea (Tables 5 and 6).


Relationship between gene expression and the accumulation of catechin during spring and autumn in tea plants (Camellia sinensis L.).

Liu M, Tian HL, Wu JH, Cang RR, Wang RX, Qi XH, Xu Q, Chen XH - Hortic Res (2015)

Expression of genes F3H and ANS encoding catechin biosynthesis enzymes in bud leaves of eight varieties of tea plants relative to β-actin gene, used as an internal control. Error bars indicate SD of three technical replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595990&req=5

fig4: Expression of genes F3H and ANS encoding catechin biosynthesis enzymes in bud leaves of eight varieties of tea plants relative to β-actin gene, used as an internal control. Error bars indicate SD of three technical replicates.
Mentions: From Tables 5 and 6, correlations between catechin content and PAL, F3H, F3′5′H, DFR, and ANS gene expression were observed in the bud leaves of eight varieties in spring and autumn. A positive correlation between TC and LAR expression (r=0.707) was observed in spring tea, but a positive correlation was noted between C4H expression and TC levels in autumn tea. F3H and ANS expression were downregulated in autumn tea as compared with spring tea, and catechin accumulation was higher in autumn tea than in spring tea (Figure 4). Catechin concentrations were negatively correlated with F3H and ANS expression levels in spring tea but positively correlated in autumn tea (Tables 5 and 6).

Bottom Line: Tea is popular worldwide given the plant's health benefits.The results indicated that the total catechin (TC) concentrations were significantly higher in tea plants harvested in autumn than in those harvested in spring, based on higher concentrations of epigallocatechin (EGC) in autumn tea (P<0.01).The expression of the genes phenylalanine ammonia-lyase (PAL), flavanone 3-hydroxylase (F3H), flavonoid 3',5'-hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) is closely related to the TC content of tea plants in both spring and autumn.

View Article: PubMed Central - PubMed

Affiliation: School of Horticulture and Plant Protection, Yangzhou University , 48 Wenhui East Road, Yangzhou, Jiangsu 225009, P. R. China.

ABSTRACT
The tea plant (Camellia sinensis L.) is an important commercial crop with remarkably high catechin concentrations. Tea is popular worldwide given the plant's health benefits. Catechins are the main astringent substance in tea and are synthesized mainly via the phenylpropanoid pathway. In this study, eight cultivars of tea plants harvested both in spring and autumn were used to investigate differences in catechin concentrations by using high-performance liquid chromatography. The expression levels of genes associated with catechin biosynthesis were investigated using reverse transcription-quantitative polymerase chain reaction. The results indicated that the total catechin (TC) concentrations were significantly higher in tea plants harvested in autumn than in those harvested in spring, based on higher concentrations of epigallocatechin (EGC) in autumn tea (P<0.01). The expression of the genes phenylalanine ammonia-lyase (PAL), flavanone 3-hydroxylase (F3H), flavonoid 3',5'-hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) is closely related to the TC content of tea plants in both spring and autumn. Positive correlations between PAL, cinnamate 4-hydroxylase (C4H), F3H, and DFR expression and EGC accumulation in autumn tea were identified, with correlation coefficients of 0.710, 0.763, 0.884, and 0.707, respectively. A negative correlation between ANS expression level and EGC concentrations in tea plants harvested in spring was noted (r=-0.732). Additionally, negative correlations between F3H and ANS expression levels and the catechin content were identified in spring tea, whereas the correlations were positive in autumn tea. Significant differences in the F3H and ANS expression levels between spring and autumn tea indicate that F3H and ANS are potentially key genes affecting catechin accumulation in tea plants.

No MeSH data available.