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Relationship between gene expression and the accumulation of catechin during spring and autumn in tea plants (Camellia sinensis L.).

Liu M, Tian HL, Wu JH, Cang RR, Wang RX, Qi XH, Xu Q, Chen XH - Hortic Res (2015)

Bottom Line: Tea is popular worldwide given the plant's health benefits.The results indicated that the total catechin (TC) concentrations were significantly higher in tea plants harvested in autumn than in those harvested in spring, based on higher concentrations of epigallocatechin (EGC) in autumn tea (P<0.01).The expression of the genes phenylalanine ammonia-lyase (PAL), flavanone 3-hydroxylase (F3H), flavonoid 3',5'-hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) is closely related to the TC content of tea plants in both spring and autumn.

View Article: PubMed Central - PubMed

Affiliation: School of Horticulture and Plant Protection, Yangzhou University , 48 Wenhui East Road, Yangzhou, Jiangsu 225009, P. R. China.

ABSTRACT
The tea plant (Camellia sinensis L.) is an important commercial crop with remarkably high catechin concentrations. Tea is popular worldwide given the plant's health benefits. Catechins are the main astringent substance in tea and are synthesized mainly via the phenylpropanoid pathway. In this study, eight cultivars of tea plants harvested both in spring and autumn were used to investigate differences in catechin concentrations by using high-performance liquid chromatography. The expression levels of genes associated with catechin biosynthesis were investigated using reverse transcription-quantitative polymerase chain reaction. The results indicated that the total catechin (TC) concentrations were significantly higher in tea plants harvested in autumn than in those harvested in spring, based on higher concentrations of epigallocatechin (EGC) in autumn tea (P<0.01). The expression of the genes phenylalanine ammonia-lyase (PAL), flavanone 3-hydroxylase (F3H), flavonoid 3',5'-hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) is closely related to the TC content of tea plants in both spring and autumn. Positive correlations between PAL, cinnamate 4-hydroxylase (C4H), F3H, and DFR expression and EGC accumulation in autumn tea were identified, with correlation coefficients of 0.710, 0.763, 0.884, and 0.707, respectively. A negative correlation between ANS expression level and EGC concentrations in tea plants harvested in spring was noted (r=-0.732). Additionally, negative correlations between F3H and ANS expression levels and the catechin content were identified in spring tea, whereas the correlations were positive in autumn tea. Significant differences in the F3H and ANS expression levels between spring and autumn tea indicate that F3H and ANS are potentially key genes affecting catechin accumulation in tea plants.

No MeSH data available.


Catechin accumulations of bud leaves in eight cultivars of tea plants harvested in spring and autumn. Error bars indicate SE of three biological replicates. Asterisks indicate that the content was significantly different (*P≤0.05, **P≤0.01).
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fig3: Catechin accumulations of bud leaves in eight cultivars of tea plants harvested in spring and autumn. Error bars indicate SE of three biological replicates. Asterisks indicate that the content was significantly different (*P≤0.05, **P≤0.01).

Mentions: Six characteristic tea catechins were successfully extracted from bud leaves (Figure 2). The most abundant catechins included the esterified catechins EGCG and ECG, which accounted for approximately 60% of the total catechin (TC), and only low amounts of non-esterified catechins were noted in spring tea (Table 3). EGCG was the most abundant catechin in tea plants harvested in spring and autumn, and EC was the least abundant (Figure 3). These results are consistent with a previous report indicating that EGCG is generally the most abundant tea catechin, whereas C and EC concentrations are rather low.40 Tea plants harvested in autumn had higher EGC concentrations, followed by EGCG, C, and GC, but lower EC and ECG concentrations than in spring tea (Figure 3). With the higher EGC concentrations, esterified catechins, including EGCG and ECG, only accounted for approximately 50% of the TC of tea plants harvested in autumn, which was 10% lower than that in spring tea (Table 3).


Relationship between gene expression and the accumulation of catechin during spring and autumn in tea plants (Camellia sinensis L.).

Liu M, Tian HL, Wu JH, Cang RR, Wang RX, Qi XH, Xu Q, Chen XH - Hortic Res (2015)

Catechin accumulations of bud leaves in eight cultivars of tea plants harvested in spring and autumn. Error bars indicate SE of three biological replicates. Asterisks indicate that the content was significantly different (*P≤0.05, **P≤0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595990&req=5

fig3: Catechin accumulations of bud leaves in eight cultivars of tea plants harvested in spring and autumn. Error bars indicate SE of three biological replicates. Asterisks indicate that the content was significantly different (*P≤0.05, **P≤0.01).
Mentions: Six characteristic tea catechins were successfully extracted from bud leaves (Figure 2). The most abundant catechins included the esterified catechins EGCG and ECG, which accounted for approximately 60% of the total catechin (TC), and only low amounts of non-esterified catechins were noted in spring tea (Table 3). EGCG was the most abundant catechin in tea plants harvested in spring and autumn, and EC was the least abundant (Figure 3). These results are consistent with a previous report indicating that EGCG is generally the most abundant tea catechin, whereas C and EC concentrations are rather low.40 Tea plants harvested in autumn had higher EGC concentrations, followed by EGCG, C, and GC, but lower EC and ECG concentrations than in spring tea (Figure 3). With the higher EGC concentrations, esterified catechins, including EGCG and ECG, only accounted for approximately 50% of the TC of tea plants harvested in autumn, which was 10% lower than that in spring tea (Table 3).

Bottom Line: Tea is popular worldwide given the plant's health benefits.The results indicated that the total catechin (TC) concentrations were significantly higher in tea plants harvested in autumn than in those harvested in spring, based on higher concentrations of epigallocatechin (EGC) in autumn tea (P<0.01).The expression of the genes phenylalanine ammonia-lyase (PAL), flavanone 3-hydroxylase (F3H), flavonoid 3',5'-hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) is closely related to the TC content of tea plants in both spring and autumn.

View Article: PubMed Central - PubMed

Affiliation: School of Horticulture and Plant Protection, Yangzhou University , 48 Wenhui East Road, Yangzhou, Jiangsu 225009, P. R. China.

ABSTRACT
The tea plant (Camellia sinensis L.) is an important commercial crop with remarkably high catechin concentrations. Tea is popular worldwide given the plant's health benefits. Catechins are the main astringent substance in tea and are synthesized mainly via the phenylpropanoid pathway. In this study, eight cultivars of tea plants harvested both in spring and autumn were used to investigate differences in catechin concentrations by using high-performance liquid chromatography. The expression levels of genes associated with catechin biosynthesis were investigated using reverse transcription-quantitative polymerase chain reaction. The results indicated that the total catechin (TC) concentrations were significantly higher in tea plants harvested in autumn than in those harvested in spring, based on higher concentrations of epigallocatechin (EGC) in autumn tea (P<0.01). The expression of the genes phenylalanine ammonia-lyase (PAL), flavanone 3-hydroxylase (F3H), flavonoid 3',5'-hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) is closely related to the TC content of tea plants in both spring and autumn. Positive correlations between PAL, cinnamate 4-hydroxylase (C4H), F3H, and DFR expression and EGC accumulation in autumn tea were identified, with correlation coefficients of 0.710, 0.763, 0.884, and 0.707, respectively. A negative correlation between ANS expression level and EGC concentrations in tea plants harvested in spring was noted (r=-0.732). Additionally, negative correlations between F3H and ANS expression levels and the catechin content were identified in spring tea, whereas the correlations were positive in autumn tea. Significant differences in the F3H and ANS expression levels between spring and autumn tea indicate that F3H and ANS are potentially key genes affecting catechin accumulation in tea plants.

No MeSH data available.