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Two quantitative trait loci, Dw1 and Dw2, are primarily responsible for rootstock-induced dwarfing in apple.

Foster TM, Celton JM, Chagné D, Tustin DS, Gardiner SE - Hortic Res (2015)

Bottom Line: The apple dwarfing rootstock 'Malling9' ('M9') has been used worldwide both to reduce scion vigour and as a genetic source for breeding new rootstocks.A smaller-effect QTL affecting dwarfing (Dw2) was identified on LG11, and four minor-effect QTLs were found on LG6, LG9, LG10 and LG12.Phenotypic analysis indicates that the combination of Dw1 and Dw2 has the strongest influence on rootstock-induced dwarfing, and that Dw1 has a stronger effect than Dw2.

View Article: PubMed Central - PubMed

Affiliation: The New Zealand Institute for Plant & Food Research Ltd , Palmerston North Private Bag 11600, Palmerston North 4474, New Zealand.

ABSTRACT
The apple dwarfing rootstock 'Malling9' ('M9') has been used worldwide both to reduce scion vigour and as a genetic source for breeding new rootstocks. Progeny of 'M9' segregate for rootstock-induced dwarfing of the scion, indicating that this trait is controlled by one or more genetic factors. A quantitative trait locus (QTL) analysis of a rootstock population derived from the cross between 'M9' × 'Robusta5' (non-dwarfing) and grafted with 'Braeburn' scions identified a major QTL (Dw1) on linkage group (LG) 5, which exhibits a significant influence on dwarfing of the scion. A smaller-effect QTL affecting dwarfing (Dw2) was identified on LG11, and four minor-effect QTLs were found on LG6, LG9, LG10 and LG12. Phenotypic analysis indicates that the combination of Dw1 and Dw2 has the strongest influence on rootstock-induced dwarfing, and that Dw1 has a stronger effect than Dw2. Genetic markers linked to Dw1 and Dw2 were screened over 41 rootstock accessions that confer a range of effects on scion growth. The majority of the dwarfing and semi-dwarfing rootstock accessions screened carried marker alleles linked to Dw1 and Dw2. This suggests that most apple dwarfing rootstocks have been derived from the same genetic source.

No MeSH data available.


Related in: MedlinePlus

Summary of Dw1 and Dw2 genotyping of rootstock accessions. A green square indicates the presence of a single allele of Dw1; yellow represents Dw2. Details on the markers employed to genotype Dw1 and Dw2 and the sizes of products amplified by each accession are specified in Supplementary Tables S1 and S3, respectively.
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fig6: Summary of Dw1 and Dw2 genotyping of rootstock accessions. A green square indicates the presence of a single allele of Dw1; yellow represents Dw2. Details on the markers employed to genotype Dw1 and Dw2 and the sizes of products amplified by each accession are specified in Supplementary Tables S1 and S3, respectively.

Mentions: To determine whether Dw1 and Dw2 are present in other known dwarfing rootstocks, we employed multi-allelic SSR markers linked to either Dw1 or Dw2 to genotype 41 rootstock accessions that confer a range of phenotypes on the scion. The use of SSR genotyping provided more detailed information about the allelic status of each locus, which cannot be determined from High Resolution Melting-based single nucleotide polymorphism markers. Three markers, Hi01c04, Hi04a08, and CH03a09, linked to Dw1 on LG5, and three markers spanning from 3.51 to 8.98 Mb on LG11 of ‘Golden Delicious’ were employed to test for the presence of Dw2 (Supplementary Table S1). Figure 6 summarizes the results of SSR genotyping for Dw1 and Dw2.


Two quantitative trait loci, Dw1 and Dw2, are primarily responsible for rootstock-induced dwarfing in apple.

Foster TM, Celton JM, Chagné D, Tustin DS, Gardiner SE - Hortic Res (2015)

Summary of Dw1 and Dw2 genotyping of rootstock accessions. A green square indicates the presence of a single allele of Dw1; yellow represents Dw2. Details on the markers employed to genotype Dw1 and Dw2 and the sizes of products amplified by each accession are specified in Supplementary Tables S1 and S3, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595989&req=5

fig6: Summary of Dw1 and Dw2 genotyping of rootstock accessions. A green square indicates the presence of a single allele of Dw1; yellow represents Dw2. Details on the markers employed to genotype Dw1 and Dw2 and the sizes of products amplified by each accession are specified in Supplementary Tables S1 and S3, respectively.
Mentions: To determine whether Dw1 and Dw2 are present in other known dwarfing rootstocks, we employed multi-allelic SSR markers linked to either Dw1 or Dw2 to genotype 41 rootstock accessions that confer a range of phenotypes on the scion. The use of SSR genotyping provided more detailed information about the allelic status of each locus, which cannot be determined from High Resolution Melting-based single nucleotide polymorphism markers. Three markers, Hi01c04, Hi04a08, and CH03a09, linked to Dw1 on LG5, and three markers spanning from 3.51 to 8.98 Mb on LG11 of ‘Golden Delicious’ were employed to test for the presence of Dw2 (Supplementary Table S1). Figure 6 summarizes the results of SSR genotyping for Dw1 and Dw2.

Bottom Line: The apple dwarfing rootstock 'Malling9' ('M9') has been used worldwide both to reduce scion vigour and as a genetic source for breeding new rootstocks.A smaller-effect QTL affecting dwarfing (Dw2) was identified on LG11, and four minor-effect QTLs were found on LG6, LG9, LG10 and LG12.Phenotypic analysis indicates that the combination of Dw1 and Dw2 has the strongest influence on rootstock-induced dwarfing, and that Dw1 has a stronger effect than Dw2.

View Article: PubMed Central - PubMed

Affiliation: The New Zealand Institute for Plant & Food Research Ltd , Palmerston North Private Bag 11600, Palmerston North 4474, New Zealand.

ABSTRACT
The apple dwarfing rootstock 'Malling9' ('M9') has been used worldwide both to reduce scion vigour and as a genetic source for breeding new rootstocks. Progeny of 'M9' segregate for rootstock-induced dwarfing of the scion, indicating that this trait is controlled by one or more genetic factors. A quantitative trait locus (QTL) analysis of a rootstock population derived from the cross between 'M9' × 'Robusta5' (non-dwarfing) and grafted with 'Braeburn' scions identified a major QTL (Dw1) on linkage group (LG) 5, which exhibits a significant influence on dwarfing of the scion. A smaller-effect QTL affecting dwarfing (Dw2) was identified on LG11, and four minor-effect QTLs were found on LG6, LG9, LG10 and LG12. Phenotypic analysis indicates that the combination of Dw1 and Dw2 has the strongest influence on rootstock-induced dwarfing, and that Dw1 has a stronger effect than Dw2. Genetic markers linked to Dw1 and Dw2 were screened over 41 rootstock accessions that confer a range of effects on scion growth. The majority of the dwarfing and semi-dwarfing rootstock accessions screened carried marker alleles linked to Dw1 and Dw2. This suggests that most apple dwarfing rootstocks have been derived from the same genetic source.

No MeSH data available.


Related in: MedlinePlus