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Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus

Representative pathways in de- and re-hydration treatments in M. flabellifolia transcript profiles. Log2 ratios are used to express relative transcript abundance at the indicated percentage of IFW during dehydration (De-) or at the indicated time of rehydration (Re-) of leaves versus hydrated leaves. Red squares indicate increased abundance and blue squares indicate decreased abundance.
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fig9: Representative pathways in de- and re-hydration treatments in M. flabellifolia transcript profiles. Log2 ratios are used to express relative transcript abundance at the indicated percentage of IFW during dehydration (De-) or at the indicated time of rehydration (Re-) of leaves versus hydrated leaves. Red squares indicate increased abundance and blue squares indicate decreased abundance.

Mentions: The plant hormone ABA plays a critical role during dehydration stress.9,43 Evidence from earlier studies showed that ABA is also important for desiccation tolerance in resurrection plants such as C. plantagineum71 and Chamaegigas intrepidus.72 ABA content increases in desiccated leaves of M. flabellifolia, and desiccation stress tolerance is enhanced by exogenous ABA treatment in M. flabellifolia.73 In agreement with the physiological studies, our desiccation transcriptomic results showed that many ABA biosynthesis and signaling genes were up-regulated during desiccation (Figure 9 and Supplementary File 10). For example, transcripts of four homologs of the nine-cis-epoxycarotenoid dioxygenase (NCED) gene (comp41624_c0_seq1, comp47700_c0_seq4, comp49970_c0_seq2, comp40276_c0_seq1, Figure 9, Supplementary File 10), encoding the rate limiting enzyme in ABA biosynthesis, were increased during dehydration. Similarly, transcripts of several ABA signaling-related genes were also up-regulated, including ABF2 (comp42333_c0_seq1), ABI1 (comp47878_c0_seq10), and HAI2 (comp48773_c0_seq8) (Figure 9 and Supplementary File 10).


Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Representative pathways in de- and re-hydration treatments in M. flabellifolia transcript profiles. Log2 ratios are used to express relative transcript abundance at the indicated percentage of IFW during dehydration (De-) or at the indicated time of rehydration (Re-) of leaves versus hydrated leaves. Red squares indicate increased abundance and blue squares indicate decreased abundance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595987&req=5

fig9: Representative pathways in de- and re-hydration treatments in M. flabellifolia transcript profiles. Log2 ratios are used to express relative transcript abundance at the indicated percentage of IFW during dehydration (De-) or at the indicated time of rehydration (Re-) of leaves versus hydrated leaves. Red squares indicate increased abundance and blue squares indicate decreased abundance.
Mentions: The plant hormone ABA plays a critical role during dehydration stress.9,43 Evidence from earlier studies showed that ABA is also important for desiccation tolerance in resurrection plants such as C. plantagineum71 and Chamaegigas intrepidus.72 ABA content increases in desiccated leaves of M. flabellifolia, and desiccation stress tolerance is enhanced by exogenous ABA treatment in M. flabellifolia.73 In agreement with the physiological studies, our desiccation transcriptomic results showed that many ABA biosynthesis and signaling genes were up-regulated during desiccation (Figure 9 and Supplementary File 10). For example, transcripts of four homologs of the nine-cis-epoxycarotenoid dioxygenase (NCED) gene (comp41624_c0_seq1, comp47700_c0_seq4, comp49970_c0_seq2, comp40276_c0_seq1, Figure 9, Supplementary File 10), encoding the rate limiting enzyme in ABA biosynthesis, were increased during dehydration. Similarly, transcripts of several ABA signaling-related genes were also up-regulated, including ABF2 (comp42333_c0_seq1), ABI1 (comp47878_c0_seq10), and HAI2 (comp48773_c0_seq8) (Figure 9 and Supplementary File 10).

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus