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Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus

Distribution of dehydration- and rehydration-responsive transcription factors and protein kinases in M. flabellifolia.
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fig6: Distribution of dehydration- and rehydration-responsive transcription factors and protein kinases in M. flabellifolia.

Mentions: Transcription factors (TFs) act as master switches of transcriptional reprogramming, inducing diverse protective mechanisms in response to abiotic stresses.9,43,44 We identified a large number of TFs (295) responsive to dehydration, and 287 TFs were responsive to rehydration from among the DTGs (Supplementary Files 2 and 6). Among these TFs, the bHLH, MYB, and WRKY families were the largest groups during both dehydration and rehydration (Figure 6). Members of these three TF families are implicated in stress responses in model plants. In Arabidopsis, bHLH-type genes AtMYC2 and AtAIB are involved in ABA signaling, and overexpression of AtMYC2 or AtAIB enhances the drought tolerance of transgenic plants.45,46 Multiple MYB genes have also been implicated in stress responses. For example, AtMYB2 is involved in the ABA-dependent drought tolerance pathway,45 and AtMYB108 is associated with both biotic and abiotic stress responses.47WRKY TFs are well known for their involvement in the regulation of plant development and in response to abiotic stresses.48,49 Functional analyses of rice OsWRKY11 and soybean GmWRKY54 demonstrated that WRKYs are involved in drought signaling pathways.50 SBP (SQUAMOSA promoter binding protein) and TCP TFs are known to be plant-specific developmental regulators.51 Our results revealed that 9 SBP and 5 TCP type TFs also showed a response to dehydration in M. flabellifolia (Figure 6 and Supplementary File 6). Further functional characterization of these TFs may shed light on their roles in desiccation tolerance.


Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Distribution of dehydration- and rehydration-responsive transcription factors and protein kinases in M. flabellifolia.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595987&req=5

fig6: Distribution of dehydration- and rehydration-responsive transcription factors and protein kinases in M. flabellifolia.
Mentions: Transcription factors (TFs) act as master switches of transcriptional reprogramming, inducing diverse protective mechanisms in response to abiotic stresses.9,43,44 We identified a large number of TFs (295) responsive to dehydration, and 287 TFs were responsive to rehydration from among the DTGs (Supplementary Files 2 and 6). Among these TFs, the bHLH, MYB, and WRKY families were the largest groups during both dehydration and rehydration (Figure 6). Members of these three TF families are implicated in stress responses in model plants. In Arabidopsis, bHLH-type genes AtMYC2 and AtAIB are involved in ABA signaling, and overexpression of AtMYC2 or AtAIB enhances the drought tolerance of transgenic plants.45,46 Multiple MYB genes have also been implicated in stress responses. For example, AtMYB2 is involved in the ABA-dependent drought tolerance pathway,45 and AtMYB108 is associated with both biotic and abiotic stress responses.47WRKY TFs are well known for their involvement in the regulation of plant development and in response to abiotic stresses.48,49 Functional analyses of rice OsWRKY11 and soybean GmWRKY54 demonstrated that WRKYs are involved in drought signaling pathways.50 SBP (SQUAMOSA promoter binding protein) and TCP TFs are known to be plant-specific developmental regulators.51 Our results revealed that 9 SBP and 5 TCP type TFs also showed a response to dehydration in M. flabellifolia (Figure 6 and Supplementary File 6). Further functional characterization of these TFs may shed light on their roles in desiccation tolerance.

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus