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Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


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Enriched pathways of DTGs during dehydration and rehydration.
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fig5: Enriched pathways of DTGs during dehydration and rehydration.

Mentions: To further delineate the metabolic pathways participating in the dehydration and rehydration responses, we mapped the DTGs into the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 23 and 18 biochemical pathways were significantly enriched during dehydration and rehydration, respectively (Figure 5). During dehydration, consistent with the GO enrichment analysis, the enriched biochemical pathways involved diverse metabolites, including secondary metabolites, starch, sucrose, fatty acids, fructose and mannose, and photosynthesis, suggesting a global response to desiccation in M. flabellifolia. As one example, the flavonoid biosynthesis pathway was significantly enriched during dehydration, consistent with the role of anthocyanins, a flavonoid class, in the ‘green-to-brown’/‘brown-to-green’ leaf color alternation during dehydration and rehydration.23


Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Enriched pathways of DTGs during dehydration and rehydration.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595987&req=5

fig5: Enriched pathways of DTGs during dehydration and rehydration.
Mentions: To further delineate the metabolic pathways participating in the dehydration and rehydration responses, we mapped the DTGs into the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 23 and 18 biochemical pathways were significantly enriched during dehydration and rehydration, respectively (Figure 5). During dehydration, consistent with the GO enrichment analysis, the enriched biochemical pathways involved diverse metabolites, including secondary metabolites, starch, sucrose, fatty acids, fructose and mannose, and photosynthesis, suggesting a global response to desiccation in M. flabellifolia. As one example, the flavonoid biosynthesis pathway was significantly enriched during dehydration, consistent with the role of anthocyanins, a flavonoid class, in the ‘green-to-brown’/‘brown-to-green’ leaf color alternation during dehydration and rehydration.23

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus