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Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus

Venn diagrams showing common genes significantly up- or down-regulated in dehydration and rehydration processes.
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fig2: Venn diagrams showing common genes significantly up- or down-regulated in dehydration and rehydration processes.

Mentions: A total of 8287 differentially transcribed genes (DTGs) were detected in the dehydration treatments (Supplementary File 4). The overlapping regions of the Venn diagram in Figure 2 show that 204 DTGs were up-regulated and 547 DTGs were down-regulated during all the dehydration stages. The number of up- and down-regulated DTGs increased as desiccation progressed (Figure 2), indicating increasing molecular responses as dehydration stress increased. During rehydration, a total of 8542 DTGs were identified (Supplementary File 5). Among these DTGs, 942 up-regulated DTGs and 3179 down-regulated DTGs were present in both the 6 h and 12 h rehydration stages (Figure 2).


Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Venn diagrams showing common genes significantly up- or down-regulated in dehydration and rehydration processes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595987&req=5

fig2: Venn diagrams showing common genes significantly up- or down-regulated in dehydration and rehydration processes.
Mentions: A total of 8287 differentially transcribed genes (DTGs) were detected in the dehydration treatments (Supplementary File 4). The overlapping regions of the Venn diagram in Figure 2 show that 204 DTGs were up-regulated and 547 DTGs were down-regulated during all the dehydration stages. The number of up- and down-regulated DTGs increased as desiccation progressed (Figure 2), indicating increasing molecular responses as dehydration stress increased. During rehydration, a total of 8542 DTGs were identified (Supplementary File 5). Among these DTGs, 942 up-regulated DTGs and 3179 down-regulated DTGs were present in both the 6 h and 12 h rehydration stages (Figure 2).

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus