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Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus

Water loss curve of M. flabellifolia. Water loss is expressed as the percentage of IFW at each time point. Results are the means of three biological replicates ± SD.
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fig1: Water loss curve of M. flabellifolia. Water loss is expressed as the percentage of IFW at each time point. Results are the means of three biological replicates ± SD.

Mentions: M. flabellifolia plants were kindly provided by Dr. Matthew Opel (University of Connecticut) and grown in containers in the greenhouse at the University of California, Davis under a 16 h/8 h day/night cycle. Current season branches from two-year-old plants were used for the treatments. For the dehydration treatment, fully hydrated branches were harvested and placed in a high humidity chamber for 30 min to reduce any potential initial wounding effects. Dehydration was performed by placing the cut branches in a temperature-controlled (20°C) room at approximately 40% relative humidity. Mature leaves were collected when the branches weighed 90% (onset of leaf wilting), 75% (leaf wilted), or 27% (desiccated) of their initial fresh weight (IFW) (Figure 1). For rehydration, desiccated branches were submerged in water for 6 h (onset of leaf unfolding) and 12 h (leaves recovered). Mature leaves from all these stages were collected and immediately frozen in liquid nitrogen and stored at −80°C until use. Three biological replicates were mixed for the preparation of an RNA-Seq library.


Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

Ma C, Wang H, Macnish AJ, Estrada-Melo AC, Lin J, Chang Y, Reid MS, Jiang CZ - Hortic Res (2015)

Water loss curve of M. flabellifolia. Water loss is expressed as the percentage of IFW at each time point. Results are the means of three biological replicates ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595987&req=5

fig1: Water loss curve of M. flabellifolia. Water loss is expressed as the percentage of IFW at each time point. Results are the means of three biological replicates ± SD.
Mentions: M. flabellifolia plants were kindly provided by Dr. Matthew Opel (University of Connecticut) and grown in containers in the greenhouse at the University of California, Davis under a 16 h/8 h day/night cycle. Current season branches from two-year-old plants were used for the treatments. For the dehydration treatment, fully hydrated branches were harvested and placed in a high humidity chamber for 30 min to reduce any potential initial wounding effects. Dehydration was performed by placing the cut branches in a temperature-controlled (20°C) room at approximately 40% relative humidity. Mature leaves were collected when the branches weighed 90% (onset of leaf wilting), 75% (leaf wilted), or 27% (desiccated) of their initial fresh weight (IFW) (Figure 1). For rehydration, desiccated branches were submerged in water for 6 h (onset of leaf unfolding) and 12 h (leaves recovered). Mature leaves from all these stages were collected and immediately frozen in liquid nitrogen and stored at −80°C until use. Three biological replicates were mixed for the preparation of an RNA-Seq library.

Bottom Line: Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation.Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation.The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California , Davis, Davis, CA 95616, USA.

ABSTRACT
The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia.

No MeSH data available.


Related in: MedlinePlus