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Morphological characteristics, anatomical structure, and gene expression: novel insights into gibberellin biosynthesis and perception during carrot growth and development.

Wang GL, Xiong F, Que F, Xu ZS, Wang F, Xiong AS - Hortic Res (2015)

Bottom Line: Carrot undergoes significant alteration in organ size during its growth and development.Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves.The results suggested that gibberellin level may play a vital role in carrot elongation and expansion.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Gibberellins (GAs) are considered potentially important regulators of cell elongation and expansion in plants. Carrot undergoes significant alteration in organ size during its growth and development. However, the molecular mechanisms underlying gibberellin accumulation and perception during carrot growth and development remain unclear. In this study, five stages of carrot growth and development were investigated using morphological and anatomical structural techniques. Gibberellin levels in leaf, petiole, and taproot tissues were also investigated for all five stages. Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves. Genes involved in gibberellin biosynthesis and signaling were identified from the carrotDB, and their expression was analyzed. All of the genes were evidently responsive to carrot growth and development, and some of them showed tissue-specific expression. The results suggested that gibberellin level may play a vital role in carrot elongation and expansion. The relative transcription levels of gibberellin pathway-related genes may be the main cause of the different bioactive GAs levels, thus exerting influences on gibberellin perception and signals. Carrot growth and development may be regulated by modification of the genes involved in gibberellin biosynthesis, catabolism, and perception.

No MeSH data available.


Related in: MedlinePlus

qRT-PCR analysis of genes involved in GA response in different tissues during carrot growth and development. Error bars represent standard deviation among three independent replicates. Data are expressed as the mean ± SD of three replicates.
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fig8: qRT-PCR analysis of genes involved in GA response in different tissues during carrot growth and development. Error bars represent standard deviation among three independent replicates. Data are expressed as the mean ± SD of three replicates.

Mentions: GA perception and subsequent signal transduction are essential for GA functions during plant growth. Thus, GA receptors should be identified to further increase our understanding of the GA signaling pathway. The genes involved in GA response, namely, GID1a, GID1b, GID1c, DcDELLA1, DcDELLA2, DcDELLA3, DcSLY1, DcCIGR, DcPICKLE1, DcPICKLE2, DcSPY, DcGAMYB, DcSHI1, DcSHI2, and DcSHI3 showed marked changes in mRNA levels during plant growth (Figure 8). In the roots, the transcript levels of DcGID1b and DcSLY1 were higher at 90 DAS and lower at 25 and 42 DAS. DcCIGR, DcSHI2, and DcSHI3 showed high expression at 25 and 42 DAS and consistently low expression at the last three time points. DcGID1a, DcGID1c, DcCIGR, DcPICKLE1, DcPICKLE2, DcSPY, DcGAMYB, and DcSHI1 were highly expressed at 60 DAS, whereas transcription of DcDELLA1 was highest at 75 DAS. In the petioles, transcription of DcDELLA3 was highest at 40 DAS. DcGID1b, DcGID1c, DcSLY1, DcCIGR, DcPICKLE2, DcSPY, and DcSHI1 showed high expression at 90 DAS. In the leaves, DcGID1c, DcDELLA1, DcDELLA2, DcCIGR, DcCIGR, DcPICKLE2, and DcSHI2 exhibited the highest mRNA abundance at 25DAS, whereas DcGID1a and DcGID1b showed high expression at 90 DAS.


Morphological characteristics, anatomical structure, and gene expression: novel insights into gibberellin biosynthesis and perception during carrot growth and development.

Wang GL, Xiong F, Que F, Xu ZS, Wang F, Xiong AS - Hortic Res (2015)

qRT-PCR analysis of genes involved in GA response in different tissues during carrot growth and development. Error bars represent standard deviation among three independent replicates. Data are expressed as the mean ± SD of three replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595985&req=5

fig8: qRT-PCR analysis of genes involved in GA response in different tissues during carrot growth and development. Error bars represent standard deviation among three independent replicates. Data are expressed as the mean ± SD of three replicates.
Mentions: GA perception and subsequent signal transduction are essential for GA functions during plant growth. Thus, GA receptors should be identified to further increase our understanding of the GA signaling pathway. The genes involved in GA response, namely, GID1a, GID1b, GID1c, DcDELLA1, DcDELLA2, DcDELLA3, DcSLY1, DcCIGR, DcPICKLE1, DcPICKLE2, DcSPY, DcGAMYB, DcSHI1, DcSHI2, and DcSHI3 showed marked changes in mRNA levels during plant growth (Figure 8). In the roots, the transcript levels of DcGID1b and DcSLY1 were higher at 90 DAS and lower at 25 and 42 DAS. DcCIGR, DcSHI2, and DcSHI3 showed high expression at 25 and 42 DAS and consistently low expression at the last three time points. DcGID1a, DcGID1c, DcCIGR, DcPICKLE1, DcPICKLE2, DcSPY, DcGAMYB, and DcSHI1 were highly expressed at 60 DAS, whereas transcription of DcDELLA1 was highest at 75 DAS. In the petioles, transcription of DcDELLA3 was highest at 40 DAS. DcGID1b, DcGID1c, DcSLY1, DcCIGR, DcPICKLE2, DcSPY, and DcSHI1 showed high expression at 90 DAS. In the leaves, DcGID1c, DcDELLA1, DcDELLA2, DcCIGR, DcCIGR, DcPICKLE2, and DcSHI2 exhibited the highest mRNA abundance at 25DAS, whereas DcGID1a and DcGID1b showed high expression at 90 DAS.

Bottom Line: Carrot undergoes significant alteration in organ size during its growth and development.Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves.The results suggested that gibberellin level may play a vital role in carrot elongation and expansion.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Gibberellins (GAs) are considered potentially important regulators of cell elongation and expansion in plants. Carrot undergoes significant alteration in organ size during its growth and development. However, the molecular mechanisms underlying gibberellin accumulation and perception during carrot growth and development remain unclear. In this study, five stages of carrot growth and development were investigated using morphological and anatomical structural techniques. Gibberellin levels in leaf, petiole, and taproot tissues were also investigated for all five stages. Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves. Genes involved in gibberellin biosynthesis and signaling were identified from the carrotDB, and their expression was analyzed. All of the genes were evidently responsive to carrot growth and development, and some of them showed tissue-specific expression. The results suggested that gibberellin level may play a vital role in carrot elongation and expansion. The relative transcription levels of gibberellin pathway-related genes may be the main cause of the different bioactive GAs levels, thus exerting influences on gibberellin perception and signals. Carrot growth and development may be regulated by modification of the genes involved in gibberellin biosynthesis, catabolism, and perception.

No MeSH data available.


Related in: MedlinePlus