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Morphological characteristics, anatomical structure, and gene expression: novel insights into gibberellin biosynthesis and perception during carrot growth and development.

Wang GL, Xiong F, Que F, Xu ZS, Wang F, Xiong AS - Hortic Res (2015)

Bottom Line: Carrot undergoes significant alteration in organ size during its growth and development.Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves.The results suggested that gibberellin level may play a vital role in carrot elongation and expansion.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Gibberellins (GAs) are considered potentially important regulators of cell elongation and expansion in plants. Carrot undergoes significant alteration in organ size during its growth and development. However, the molecular mechanisms underlying gibberellin accumulation and perception during carrot growth and development remain unclear. In this study, five stages of carrot growth and development were investigated using morphological and anatomical structural techniques. Gibberellin levels in leaf, petiole, and taproot tissues were also investigated for all five stages. Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves. Genes involved in gibberellin biosynthesis and signaling were identified from the carrotDB, and their expression was analyzed. All of the genes were evidently responsive to carrot growth and development, and some of them showed tissue-specific expression. The results suggested that gibberellin level may play a vital role in carrot elongation and expansion. The relative transcription levels of gibberellin pathway-related genes may be the main cause of the different bioactive GAs levels, thus exerting influences on gibberellin perception and signals. Carrot growth and development may be regulated by modification of the genes involved in gibberellin biosynthesis, catabolism, and perception.

No MeSH data available.


Related in: MedlinePlus

Bioactive GAs levels in different tissues during carrot growth and development. Error bars represent the standard deviation among three independent replicates. Data are expressed as the mean ± SD of three replicates. Different lowercase letters represent significant differences at P <0.05.
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fig6: Bioactive GAs levels in different tissues during carrot growth and development. Error bars represent the standard deviation among three independent replicates. Data are expressed as the mean ± SD of three replicates. Different lowercase letters represent significant differences at P <0.05.

Mentions: The levels of bioactive GAs (GA1, GA3, GA4, and GA7) were analyzed in the roots, petioles, and leaves during carrot growth and development (Figure 6). For each plant, the bioactive GA contents in the leaves and petioles were higher than those in the roots. During carrot growth, the highest GA levels in the roots were observed at 42 DAS; this level subsequently decreased. However, GA levels in the petioles slightly changed, and the GA levels in the leaves relatively fluctuated.


Morphological characteristics, anatomical structure, and gene expression: novel insights into gibberellin biosynthesis and perception during carrot growth and development.

Wang GL, Xiong F, Que F, Xu ZS, Wang F, Xiong AS - Hortic Res (2015)

Bioactive GAs levels in different tissues during carrot growth and development. Error bars represent the standard deviation among three independent replicates. Data are expressed as the mean ± SD of three replicates. Different lowercase letters represent significant differences at P <0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595985&req=5

fig6: Bioactive GAs levels in different tissues during carrot growth and development. Error bars represent the standard deviation among three independent replicates. Data are expressed as the mean ± SD of three replicates. Different lowercase letters represent significant differences at P <0.05.
Mentions: The levels of bioactive GAs (GA1, GA3, GA4, and GA7) were analyzed in the roots, petioles, and leaves during carrot growth and development (Figure 6). For each plant, the bioactive GA contents in the leaves and petioles were higher than those in the roots. During carrot growth, the highest GA levels in the roots were observed at 42 DAS; this level subsequently decreased. However, GA levels in the petioles slightly changed, and the GA levels in the leaves relatively fluctuated.

Bottom Line: Carrot undergoes significant alteration in organ size during its growth and development.Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves.The results suggested that gibberellin level may play a vital role in carrot elongation and expansion.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Gibberellins (GAs) are considered potentially important regulators of cell elongation and expansion in plants. Carrot undergoes significant alteration in organ size during its growth and development. However, the molecular mechanisms underlying gibberellin accumulation and perception during carrot growth and development remain unclear. In this study, five stages of carrot growth and development were investigated using morphological and anatomical structural techniques. Gibberellin levels in leaf, petiole, and taproot tissues were also investigated for all five stages. Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves. Genes involved in gibberellin biosynthesis and signaling were identified from the carrotDB, and their expression was analyzed. All of the genes were evidently responsive to carrot growth and development, and some of them showed tissue-specific expression. The results suggested that gibberellin level may play a vital role in carrot elongation and expansion. The relative transcription levels of gibberellin pathway-related genes may be the main cause of the different bioactive GAs levels, thus exerting influences on gibberellin perception and signals. Carrot growth and development may be regulated by modification of the genes involved in gibberellin biosynthesis, catabolism, and perception.

No MeSH data available.


Related in: MedlinePlus