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Morphological characteristics, anatomical structure, and gene expression: novel insights into gibberellin biosynthesis and perception during carrot growth and development.

Wang GL, Xiong F, Que F, Xu ZS, Wang F, Xiong AS - Hortic Res (2015)

Bottom Line: Carrot undergoes significant alteration in organ size during its growth and development.Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves.The results suggested that gibberellin level may play a vital role in carrot elongation and expansion.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Gibberellins (GAs) are considered potentially important regulators of cell elongation and expansion in plants. Carrot undergoes significant alteration in organ size during its growth and development. However, the molecular mechanisms underlying gibberellin accumulation and perception during carrot growth and development remain unclear. In this study, five stages of carrot growth and development were investigated using morphological and anatomical structural techniques. Gibberellin levels in leaf, petiole, and taproot tissues were also investigated for all five stages. Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves. Genes involved in gibberellin biosynthesis and signaling were identified from the carrotDB, and their expression was analyzed. All of the genes were evidently responsive to carrot growth and development, and some of them showed tissue-specific expression. The results suggested that gibberellin level may play a vital role in carrot elongation and expansion. The relative transcription levels of gibberellin pathway-related genes may be the main cause of the different bioactive GAs levels, thus exerting influences on gibberellin perception and signals. Carrot growth and development may be regulated by modification of the genes involved in gibberellin biosynthesis, catabolism, and perception.

No MeSH data available.


Structural changes in the petioles during carrot growth and development. The petioles were harvested at 25 (A), 42 (B), 60 (C), 75 (D), and 90 (E) days after sowing. Collenchyma (Co), epidermis (Ep), exodermis (Ex), phloem (P), and xylem (X) were marked in the figure. Scale bars in A, B, C, D, and E are 100 μm in length.
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fig4: Structural changes in the petioles during carrot growth and development. The petioles were harvested at 25 (A), 42 (B), 60 (C), 75 (D), and 90 (E) days after sowing. Collenchyma (Co), epidermis (Ep), exodermis (Ex), phloem (P), and xylem (X) were marked in the figure. Scale bars in A, B, C, D, and E are 100 μm in length.

Mentions: The collenchyma (Co), which provides structural support for carrot growth, was very conspicuous under the microscope (Figure 4). As the plant grew, regions of Co, phloem (P), and xylem(X) were evidently enlarged, suggesting that constant thickness developed in the petioles.


Morphological characteristics, anatomical structure, and gene expression: novel insights into gibberellin biosynthesis and perception during carrot growth and development.

Wang GL, Xiong F, Que F, Xu ZS, Wang F, Xiong AS - Hortic Res (2015)

Structural changes in the petioles during carrot growth and development. The petioles were harvested at 25 (A), 42 (B), 60 (C), 75 (D), and 90 (E) days after sowing. Collenchyma (Co), epidermis (Ep), exodermis (Ex), phloem (P), and xylem (X) were marked in the figure. Scale bars in A, B, C, D, and E are 100 μm in length.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595985&req=5

fig4: Structural changes in the petioles during carrot growth and development. The petioles were harvested at 25 (A), 42 (B), 60 (C), 75 (D), and 90 (E) days after sowing. Collenchyma (Co), epidermis (Ep), exodermis (Ex), phloem (P), and xylem (X) were marked in the figure. Scale bars in A, B, C, D, and E are 100 μm in length.
Mentions: The collenchyma (Co), which provides structural support for carrot growth, was very conspicuous under the microscope (Figure 4). As the plant grew, regions of Co, phloem (P), and xylem(X) were evidently enlarged, suggesting that constant thickness developed in the petioles.

Bottom Line: Carrot undergoes significant alteration in organ size during its growth and development.Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves.The results suggested that gibberellin level may play a vital role in carrot elongation and expansion.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Gibberellins (GAs) are considered potentially important regulators of cell elongation and expansion in plants. Carrot undergoes significant alteration in organ size during its growth and development. However, the molecular mechanisms underlying gibberellin accumulation and perception during carrot growth and development remain unclear. In this study, five stages of carrot growth and development were investigated using morphological and anatomical structural techniques. Gibberellin levels in leaf, petiole, and taproot tissues were also investigated for all five stages. Gibberellin levels in the roots initially increased and then decreased, but these levels were lower than those in the petioles and leaves. Genes involved in gibberellin biosynthesis and signaling were identified from the carrotDB, and their expression was analyzed. All of the genes were evidently responsive to carrot growth and development, and some of them showed tissue-specific expression. The results suggested that gibberellin level may play a vital role in carrot elongation and expansion. The relative transcription levels of gibberellin pathway-related genes may be the main cause of the different bioactive GAs levels, thus exerting influences on gibberellin perception and signals. Carrot growth and development may be regulated by modification of the genes involved in gibberellin biosynthesis, catabolism, and perception.

No MeSH data available.