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Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry.

Ordaz-Ortiz JJ, Foukaraki S, Terry LA - Hortic Res (2015)

Bottom Line: Sylvana and Russet Burbank stored for up to 30 weeks at 6 °C under ambient air conditions.The method achieved a lowest limit of detection of 0.22 ng g(-1) of dry weight and a limit of quantification of 0.74 ng g(-1) dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers.In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

View Article: PubMed Central - PubMed

Affiliation: Plant Science Laboratory, Cranfield University , Bedfordshire, MK43 0AL, UK.

ABSTRACT
Plant hormones are important molecules which at low concentration can regulate various physiological processes. Mass spectrometry has become a powerful technique for the quantification of multiple classes of plant hormones because of its high sensitivity and selectivity. We developed a new ultrahigh pressure liquid chromatography-full-scan high-definition accurate mass spectrometry method, for simultaneous determination of abscisic acid and four metabolites phaseic acid, dihydrophaseic acid, 7'-hydroxy-abscisic acid and abscisic acid glucose ester, cytokinins zeatin, zeatin riboside, gibberellins (GA1, GA3, GA4 and GA7) and indole-3-acetyl-L-aspartic acid. We measured the amount of plant hormones in the flesh and skin of two processing potato cvs. Sylvana and Russet Burbank stored for up to 30 weeks at 6 °C under ambient air conditions. Herein, we report for the first time that abscisic acid glucose ester seems to accumulate in the skin of potato tubers throughout storage time. The method achieved a lowest limit of detection of 0.22 ng g(-1) of dry weight and a limit of quantification of 0.74 ng g(-1) dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers. In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

No MeSH data available.


Abscisic acid, Abscisic acid-glucose ester, dihydrophaseic and phaseic acid content (ng g−1 DW) in flesh and skin of potato cv. Russet Burbank tubers stored under ambient air and sampled at five time points: after harvest, at 6 weeks storage, at first indication of sprouting (red arrow), 4 weeks later and at 30 weeks storage (n=3). LSD bars for flesh (A), skin (B) and flesh and skin (C) (P<0.05) are shown.
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fig3: Abscisic acid, Abscisic acid-glucose ester, dihydrophaseic and phaseic acid content (ng g−1 DW) in flesh and skin of potato cv. Russet Burbank tubers stored under ambient air and sampled at five time points: after harvest, at 6 weeks storage, at first indication of sprouting (red arrow), 4 weeks later and at 30 weeks storage (n=3). LSD bars for flesh (A), skin (B) and flesh and skin (C) (P<0.05) are shown.

Mentions: The endogenous ABA content in skin tissue of cv. Sylvana varied from 24 ng g−1 dry weight (DW) at harvest to less than 1 ng g−1 DW at the end of 12 weeks storage corresponding to first indication of sprouting (Figure 2). These levels were slightly different for cv. Russet Burbank which varied from 24 ng g−1 DW at 6 weeks storage to 14 ng g−1 DW at 14 weeks of storage at time of first indication of sprouting (Figure 3). For both cultivars, no significant differences were observed in the content of ABA between flesh tissue compared to skin tissue throughout storage (P<0.05).


Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry.

Ordaz-Ortiz JJ, Foukaraki S, Terry LA - Hortic Res (2015)

Abscisic acid, Abscisic acid-glucose ester, dihydrophaseic and phaseic acid content (ng g−1 DW) in flesh and skin of potato cv. Russet Burbank tubers stored under ambient air and sampled at five time points: after harvest, at 6 weeks storage, at first indication of sprouting (red arrow), 4 weeks later and at 30 weeks storage (n=3). LSD bars for flesh (A), skin (B) and flesh and skin (C) (P<0.05) are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595984&req=5

fig3: Abscisic acid, Abscisic acid-glucose ester, dihydrophaseic and phaseic acid content (ng g−1 DW) in flesh and skin of potato cv. Russet Burbank tubers stored under ambient air and sampled at five time points: after harvest, at 6 weeks storage, at first indication of sprouting (red arrow), 4 weeks later and at 30 weeks storage (n=3). LSD bars for flesh (A), skin (B) and flesh and skin (C) (P<0.05) are shown.
Mentions: The endogenous ABA content in skin tissue of cv. Sylvana varied from 24 ng g−1 dry weight (DW) at harvest to less than 1 ng g−1 DW at the end of 12 weeks storage corresponding to first indication of sprouting (Figure 2). These levels were slightly different for cv. Russet Burbank which varied from 24 ng g−1 DW at 6 weeks storage to 14 ng g−1 DW at 14 weeks of storage at time of first indication of sprouting (Figure 3). For both cultivars, no significant differences were observed in the content of ABA between flesh tissue compared to skin tissue throughout storage (P<0.05).

Bottom Line: Sylvana and Russet Burbank stored for up to 30 weeks at 6 °C under ambient air conditions.The method achieved a lowest limit of detection of 0.22 ng g(-1) of dry weight and a limit of quantification of 0.74 ng g(-1) dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers.In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

View Article: PubMed Central - PubMed

Affiliation: Plant Science Laboratory, Cranfield University , Bedfordshire, MK43 0AL, UK.

ABSTRACT
Plant hormones are important molecules which at low concentration can regulate various physiological processes. Mass spectrometry has become a powerful technique for the quantification of multiple classes of plant hormones because of its high sensitivity and selectivity. We developed a new ultrahigh pressure liquid chromatography-full-scan high-definition accurate mass spectrometry method, for simultaneous determination of abscisic acid and four metabolites phaseic acid, dihydrophaseic acid, 7'-hydroxy-abscisic acid and abscisic acid glucose ester, cytokinins zeatin, zeatin riboside, gibberellins (GA1, GA3, GA4 and GA7) and indole-3-acetyl-L-aspartic acid. We measured the amount of plant hormones in the flesh and skin of two processing potato cvs. Sylvana and Russet Burbank stored for up to 30 weeks at 6 °C under ambient air conditions. Herein, we report for the first time that abscisic acid glucose ester seems to accumulate in the skin of potato tubers throughout storage time. The method achieved a lowest limit of detection of 0.22 ng g(-1) of dry weight and a limit of quantification of 0.74 ng g(-1) dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers. In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

No MeSH data available.