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Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry.

Ordaz-Ortiz JJ, Foukaraki S, Terry LA - Hortic Res (2015)

Bottom Line: We measured the amount of plant hormones in the flesh and skin of two processing potato cvs.The method achieved a lowest limit of detection of 0.22 ng g(-1) of dry weight and a limit of quantification of 0.74 ng g(-1) dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers.In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

View Article: PubMed Central - PubMed

Affiliation: Plant Science Laboratory, Cranfield University , Bedfordshire, MK43 0AL, UK.

ABSTRACT
Plant hormones are important molecules which at low concentration can regulate various physiological processes. Mass spectrometry has become a powerful technique for the quantification of multiple classes of plant hormones because of its high sensitivity and selectivity. We developed a new ultrahigh pressure liquid chromatography-full-scan high-definition accurate mass spectrometry method, for simultaneous determination of abscisic acid and four metabolites phaseic acid, dihydrophaseic acid, 7'-hydroxy-abscisic acid and abscisic acid glucose ester, cytokinins zeatin, zeatin riboside, gibberellins (GA1, GA3, GA4 and GA7) and indole-3-acetyl-L-aspartic acid. We measured the amount of plant hormones in the flesh and skin of two processing potato cvs. Sylvana and Russet Burbank stored for up to 30 weeks at 6 °C under ambient air conditions. Herein, we report for the first time that abscisic acid glucose ester seems to accumulate in the skin of potato tubers throughout storage time. The method achieved a lowest limit of detection of 0.22 ng g(-1) of dry weight and a limit of quantification of 0.74 ng g(-1) dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers. In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

No MeSH data available.


Related in: MedlinePlus

Abscisic acid, Abscisic acid-glucose ester, dihydrophaseic and phaseic acid content (ng g−1 DW) in flesh and skin of potato cv. Sylvana tubers stored under ambient air and sampled at five (flesh) or four (skin) time points: after harvest, at 6 weeks storage, at first indication of sprouting (red arrow), 4 weeks later and at 30 weeks storage (n=3). LSD bars for flesh (A) and skin (B) (P<0.05) are shown.
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fig2: Abscisic acid, Abscisic acid-glucose ester, dihydrophaseic and phaseic acid content (ng g−1 DW) in flesh and skin of potato cv. Sylvana tubers stored under ambient air and sampled at five (flesh) or four (skin) time points: after harvest, at 6 weeks storage, at first indication of sprouting (red arrow), 4 weeks later and at 30 weeks storage (n=3). LSD bars for flesh (A) and skin (B) (P<0.05) are shown.

Mentions: Potato cvs. Sylvana (Greenvale AP Ltd, Cambs, UK) and Russet Burbank (McCain Foods Ltd, Yorks, UK) were used in this study. The selected potato cultivars are categorized as maincrop (‘Russet Burbank’) and early maincrop (‘Sylvana’) according to the British Potato Variety Database (2009). These varieties arrived at Sutton Bridge Crop Storage Research facilities on 10 October 2008 and were passed over a grading line to remove loose soil, rotten, damaged, green and/or undersized (<45 mm) tubers. Sprout suppressants were not applied to the tubers used in the study. On arrival, tubers underwent a controlled cooling regime from 15 °C (arrival temperature), at a rate of 0.5 °C reduction per day at ambient relative humidity, to a holding temperature of 6 °C, in order to minimize chilling stress and allow time for wound healing. After reaching 6 °C, tubers were stored under continuous ambient air for up to 30 weeks. Tubers (n=3) were collected at five sampling points throughout storage: (i) at harvest; (ii) at 6 weeks storage; (iii) at the time of first indication of sprouting (10% of air-stored tubers showing white sprout tissue development12–14); (iv) 4 weeks later; and (v) at 30 weeks storage. Flesh and skin tissues from both cultivars were analyzed in all sampling points; however due to the insufficient amount of skin sample in potato cv. Sylvana at 30 weeks storage, those skin samples were not analyzed at that time point (Figure 2).


Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry.

Ordaz-Ortiz JJ, Foukaraki S, Terry LA - Hortic Res (2015)

Abscisic acid, Abscisic acid-glucose ester, dihydrophaseic and phaseic acid content (ng g−1 DW) in flesh and skin of potato cv. Sylvana tubers stored under ambient air and sampled at five (flesh) or four (skin) time points: after harvest, at 6 weeks storage, at first indication of sprouting (red arrow), 4 weeks later and at 30 weeks storage (n=3). LSD bars for flesh (A) and skin (B) (P<0.05) are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595984&req=5

fig2: Abscisic acid, Abscisic acid-glucose ester, dihydrophaseic and phaseic acid content (ng g−1 DW) in flesh and skin of potato cv. Sylvana tubers stored under ambient air and sampled at five (flesh) or four (skin) time points: after harvest, at 6 weeks storage, at first indication of sprouting (red arrow), 4 weeks later and at 30 weeks storage (n=3). LSD bars for flesh (A) and skin (B) (P<0.05) are shown.
Mentions: Potato cvs. Sylvana (Greenvale AP Ltd, Cambs, UK) and Russet Burbank (McCain Foods Ltd, Yorks, UK) were used in this study. The selected potato cultivars are categorized as maincrop (‘Russet Burbank’) and early maincrop (‘Sylvana’) according to the British Potato Variety Database (2009). These varieties arrived at Sutton Bridge Crop Storage Research facilities on 10 October 2008 and were passed over a grading line to remove loose soil, rotten, damaged, green and/or undersized (<45 mm) tubers. Sprout suppressants were not applied to the tubers used in the study. On arrival, tubers underwent a controlled cooling regime from 15 °C (arrival temperature), at a rate of 0.5 °C reduction per day at ambient relative humidity, to a holding temperature of 6 °C, in order to minimize chilling stress and allow time for wound healing. After reaching 6 °C, tubers were stored under continuous ambient air for up to 30 weeks. Tubers (n=3) were collected at five sampling points throughout storage: (i) at harvest; (ii) at 6 weeks storage; (iii) at the time of first indication of sprouting (10% of air-stored tubers showing white sprout tissue development12–14); (iv) 4 weeks later; and (v) at 30 weeks storage. Flesh and skin tissues from both cultivars were analyzed in all sampling points; however due to the insufficient amount of skin sample in potato cv. Sylvana at 30 weeks storage, those skin samples were not analyzed at that time point (Figure 2).

Bottom Line: We measured the amount of plant hormones in the flesh and skin of two processing potato cvs.The method achieved a lowest limit of detection of 0.22 ng g(-1) of dry weight and a limit of quantification of 0.74 ng g(-1) dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers.In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

View Article: PubMed Central - PubMed

Affiliation: Plant Science Laboratory, Cranfield University , Bedfordshire, MK43 0AL, UK.

ABSTRACT
Plant hormones are important molecules which at low concentration can regulate various physiological processes. Mass spectrometry has become a powerful technique for the quantification of multiple classes of plant hormones because of its high sensitivity and selectivity. We developed a new ultrahigh pressure liquid chromatography-full-scan high-definition accurate mass spectrometry method, for simultaneous determination of abscisic acid and four metabolites phaseic acid, dihydrophaseic acid, 7'-hydroxy-abscisic acid and abscisic acid glucose ester, cytokinins zeatin, zeatin riboside, gibberellins (GA1, GA3, GA4 and GA7) and indole-3-acetyl-L-aspartic acid. We measured the amount of plant hormones in the flesh and skin of two processing potato cvs. Sylvana and Russet Burbank stored for up to 30 weeks at 6 °C under ambient air conditions. Herein, we report for the first time that abscisic acid glucose ester seems to accumulate in the skin of potato tubers throughout storage time. The method achieved a lowest limit of detection of 0.22 ng g(-1) of dry weight and a limit of quantification of 0.74 ng g(-1) dry weight (zeatin riboside), and was able to recover, detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato tubers. In addition, the mass accuracy for all compounds (<5 ppm) was evaluated.

No MeSH data available.


Related in: MedlinePlus