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Overexpression of an ABA biosynthesis gene using a stress-inducible promoter enhances drought resistance in petunia.

Estrada-Melo AC - Hortic Res (2015)

Bottom Line: The response of plants to drought stress includes reduced transpiration as stomates close in response to increased abscisic acid (ABA) concentrations.Constitutive overexpression of 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in ABA biosynthesis, increases drought resistance, but causes negative pleiotropic effects on plant growth and development.Well-watered transgenic plants grew like non-transformed control plants and there was no effect of the transgene on seed dormancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California Davis , Davis, CA, USA.

ABSTRACT
The response of plants to drought stress includes reduced transpiration as stomates close in response to increased abscisic acid (ABA) concentrations. Constitutive overexpression of 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in ABA biosynthesis, increases drought resistance, but causes negative pleiotropic effects on plant growth and development. We overexpressed the tomato NCED (LeNCED1) in petunia plants under the control of a stress-inducible promoter, rd29A. Under water stress, the transgenic plants had increased transcripts of NCED mRNA, elevated leaf ABA concentrations, increased concentrations of proline, and a significant increase in drought resistance. The transgenic plants also displayed the expected decreases in stomatal conductance, transpiration, and photosynthesis. After 14 days without water, the control plants were dead, but the transgenic plants, though wilted, recovered fully when re-watered. Well-watered transgenic plants grew like non-transformed control plants and there was no effect of the transgene on seed dormancy.

No MeSH data available.


Related in: MedlinePlus

Drought stress resistance of rd29:LeNCED petunia. Plants of non-transformed control and transgenic rd29A:LeNCED1 line 8F were grown in pots filled with same amounts of UC Mix under non-stress conditions for 5 weeks and were subjected to drought stress by withholding water for 14 days. The control plants started wilting at day 3 and were severely wilted by day 10. In comparison, transgenic plants started to show wilting at day 10. The stressed plants were rehydrated to allow recovery and photographs were taken 10 days after re-watering. The number of surviving plants (out of the total tested) was indicated on the upper right of the image.
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fig3: Drought stress resistance of rd29:LeNCED petunia. Plants of non-transformed control and transgenic rd29A:LeNCED1 line 8F were grown in pots filled with same amounts of UC Mix under non-stress conditions for 5 weeks and were subjected to drought stress by withholding water for 14 days. The control plants started wilting at day 3 and were severely wilted by day 10. In comparison, transgenic plants started to show wilting at day 10. The stressed plants were rehydrated to allow recovery and photographs were taken 10 days after re-watering. The number of surviving plants (out of the total tested) was indicated on the upper right of the image.

Mentions: The recovery experiment compared control and rd29A:LeNCED1-8F plants. Control plants started to wilt at 3 days of drought (Figure 3) and were severely wilted by 10 days. At that time the transgenic plants showed wilting and yellowing only of the older (basal) leaves (Figure 3). After 2 weeks without water, the control plants were completely desiccated, and some transgenic plants were severely wilted, although their apices remained turgid. The plants were re-watered after 2 weeks of drought stress (Figure 3). Only 11% of the control plants survived, whereas 94.4% of the rd29A:LeNCED1 transgenic plants recovered completely.


Overexpression of an ABA biosynthesis gene using a stress-inducible promoter enhances drought resistance in petunia.

Estrada-Melo AC - Hortic Res (2015)

Drought stress resistance of rd29:LeNCED petunia. Plants of non-transformed control and transgenic rd29A:LeNCED1 line 8F were grown in pots filled with same amounts of UC Mix under non-stress conditions for 5 weeks and were subjected to drought stress by withholding water for 14 days. The control plants started wilting at day 3 and were severely wilted by day 10. In comparison, transgenic plants started to show wilting at day 10. The stressed plants were rehydrated to allow recovery and photographs were taken 10 days after re-watering. The number of surviving plants (out of the total tested) was indicated on the upper right of the image.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595983&req=5

fig3: Drought stress resistance of rd29:LeNCED petunia. Plants of non-transformed control and transgenic rd29A:LeNCED1 line 8F were grown in pots filled with same amounts of UC Mix under non-stress conditions for 5 weeks and were subjected to drought stress by withholding water for 14 days. The control plants started wilting at day 3 and were severely wilted by day 10. In comparison, transgenic plants started to show wilting at day 10. The stressed plants were rehydrated to allow recovery and photographs were taken 10 days after re-watering. The number of surviving plants (out of the total tested) was indicated on the upper right of the image.
Mentions: The recovery experiment compared control and rd29A:LeNCED1-8F plants. Control plants started to wilt at 3 days of drought (Figure 3) and were severely wilted by 10 days. At that time the transgenic plants showed wilting and yellowing only of the older (basal) leaves (Figure 3). After 2 weeks without water, the control plants were completely desiccated, and some transgenic plants were severely wilted, although their apices remained turgid. The plants were re-watered after 2 weeks of drought stress (Figure 3). Only 11% of the control plants survived, whereas 94.4% of the rd29A:LeNCED1 transgenic plants recovered completely.

Bottom Line: The response of plants to drought stress includes reduced transpiration as stomates close in response to increased abscisic acid (ABA) concentrations.Constitutive overexpression of 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in ABA biosynthesis, increases drought resistance, but causes negative pleiotropic effects on plant growth and development.Well-watered transgenic plants grew like non-transformed control plants and there was no effect of the transgene on seed dormancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California Davis , Davis, CA, USA.

ABSTRACT
The response of plants to drought stress includes reduced transpiration as stomates close in response to increased abscisic acid (ABA) concentrations. Constitutive overexpression of 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in ABA biosynthesis, increases drought resistance, but causes negative pleiotropic effects on plant growth and development. We overexpressed the tomato NCED (LeNCED1) in petunia plants under the control of a stress-inducible promoter, rd29A. Under water stress, the transgenic plants had increased transcripts of NCED mRNA, elevated leaf ABA concentrations, increased concentrations of proline, and a significant increase in drought resistance. The transgenic plants also displayed the expected decreases in stomatal conductance, transpiration, and photosynthesis. After 14 days without water, the control plants were dead, but the transgenic plants, though wilted, recovered fully when re-watered. Well-watered transgenic plants grew like non-transformed control plants and there was no effect of the transgene on seed dormancy.

No MeSH data available.


Related in: MedlinePlus