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Water limitation and rootstock genotype interact to alter grape berry metabolism through transcriptome reprogramming.

Berdeja M, Nicolas P, Kappel C, Dai ZW, Hilbert G, Peccoux A, Lafontaine M, Ollat N, Gomès E, Delrot S - Hortic Res (2015)

Bottom Line: Pinot noir) from vines grafted on either 110R (drought-tolerant) or 125AA (drought-sensitive) rootstock were compared.Indeed, under drought stress, berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate (JA), phenylpropanoid metabolism, and pathogenesis-related proteins.These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

View Article: PubMed Central - PubMed

Affiliation: Ecophysiologie et Génomique Fonctionnelle de la Vigne, University Bordeaux, ISVV, UMR 1287 , Villenave d'Ornon F-33140, France.

ABSTRACT
Grapevine is a perennial crop often cultivated by grafting a scion cultivar on a suitable rootstock. Rootstocks influence scions, particularly with regard to water uptake and vigor. Therefore, one of the possibilities to adapt viticulture to the extended drought stress periods is to select rootstocks conferring increased tolerance to drought. However, the molecular mechanisms associated with the ability of rootstock/scion combination to influence grape berry metabolism under drought stress are still poorly understood. The transcriptomic changes induced by drought stress in grape berries (cv. Pinot noir) from vines grafted on either 110R (drought-tolerant) or 125AA (drought-sensitive) rootstock were compared. The experiments were conducted in the vineyard for two years and two grape berry developmental stages (50% and 100% veraison). The genome-wide microarray approach showed that water stress strongly impacts gene expression in the berries, through ontology categories that cover cell wall metabolism, primary and secondary metabolism, signaling, stress, and hormones, and that some of these effects strongly depend on the rootstock genotype. Indeed, under drought stress, berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate (JA), phenylpropanoid metabolism, and pathogenesis-related proteins. The data also suggest a link between JA and secondary metabolism in water-stressed berries. Overall, genes related to secondary metabolism and JA are more induced and/or less repressed by drought stress in the berries grafted on the drought-sensitive rootstock 125AA. These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

No MeSH data available.


Related in: MedlinePlus

Pageman visualization of MapMan functional categories: secondary metabolism (A), hormone metabolism (B), stress (C), and amino acid metabolism (D), enriched in the genes differentially expressed between berries grafted on 110R and berries grafted on 125AA, under drought stress. The degree of enrichment of functional categories in up- and downregulated genes for the log2(110R-S/110R-C) – log2(125AA-S/125AA-C) condition is given by shades of red and blue, respectively.
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fig2: Pageman visualization of MapMan functional categories: secondary metabolism (A), hormone metabolism (B), stress (C), and amino acid metabolism (D), enriched in the genes differentially expressed between berries grafted on 110R and berries grafted on 125AA, under drought stress. The degree of enrichment of functional categories in up- and downregulated genes for the log2(110R-S/110R-C) – log2(125AA-S/125AA-C) condition is given by shades of red and blue, respectively.

Mentions: Our transcriptomic data agree with previous observations according to which water deficit affects phenylpropanoid metabolism and increases the berry concentration in anthocyanins and other phenolics.10,11,40 Furthermore, we show that the effects of water deficit on the expression of genes of this family in the berries depend on the rootstock. Indeed, the Pageman representation shows that, overall, genes from the functional category ‘secondary metabolism’ (more particularly phenylpropanoids and flavonoids) are more induced or less repressed by drought stress in berries grown on the drought-sensitive rootstock 125AA compared to the drought-tolerant rootstock 110R, except for E-L 36 developmental stage in 2009 (Figure 2A).


Water limitation and rootstock genotype interact to alter grape berry metabolism through transcriptome reprogramming.

Berdeja M, Nicolas P, Kappel C, Dai ZW, Hilbert G, Peccoux A, Lafontaine M, Ollat N, Gomès E, Delrot S - Hortic Res (2015)

Pageman visualization of MapMan functional categories: secondary metabolism (A), hormone metabolism (B), stress (C), and amino acid metabolism (D), enriched in the genes differentially expressed between berries grafted on 110R and berries grafted on 125AA, under drought stress. The degree of enrichment of functional categories in up- and downregulated genes for the log2(110R-S/110R-C) – log2(125AA-S/125AA-C) condition is given by shades of red and blue, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595978&req=5

fig2: Pageman visualization of MapMan functional categories: secondary metabolism (A), hormone metabolism (B), stress (C), and amino acid metabolism (D), enriched in the genes differentially expressed between berries grafted on 110R and berries grafted on 125AA, under drought stress. The degree of enrichment of functional categories in up- and downregulated genes for the log2(110R-S/110R-C) – log2(125AA-S/125AA-C) condition is given by shades of red and blue, respectively.
Mentions: Our transcriptomic data agree with previous observations according to which water deficit affects phenylpropanoid metabolism and increases the berry concentration in anthocyanins and other phenolics.10,11,40 Furthermore, we show that the effects of water deficit on the expression of genes of this family in the berries depend on the rootstock. Indeed, the Pageman representation shows that, overall, genes from the functional category ‘secondary metabolism’ (more particularly phenylpropanoids and flavonoids) are more induced or less repressed by drought stress in berries grown on the drought-sensitive rootstock 125AA compared to the drought-tolerant rootstock 110R, except for E-L 36 developmental stage in 2009 (Figure 2A).

Bottom Line: Pinot noir) from vines grafted on either 110R (drought-tolerant) or 125AA (drought-sensitive) rootstock were compared.Indeed, under drought stress, berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate (JA), phenylpropanoid metabolism, and pathogenesis-related proteins.These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

View Article: PubMed Central - PubMed

Affiliation: Ecophysiologie et Génomique Fonctionnelle de la Vigne, University Bordeaux, ISVV, UMR 1287 , Villenave d'Ornon F-33140, France.

ABSTRACT
Grapevine is a perennial crop often cultivated by grafting a scion cultivar on a suitable rootstock. Rootstocks influence scions, particularly with regard to water uptake and vigor. Therefore, one of the possibilities to adapt viticulture to the extended drought stress periods is to select rootstocks conferring increased tolerance to drought. However, the molecular mechanisms associated with the ability of rootstock/scion combination to influence grape berry metabolism under drought stress are still poorly understood. The transcriptomic changes induced by drought stress in grape berries (cv. Pinot noir) from vines grafted on either 110R (drought-tolerant) or 125AA (drought-sensitive) rootstock were compared. The experiments were conducted in the vineyard for two years and two grape berry developmental stages (50% and 100% veraison). The genome-wide microarray approach showed that water stress strongly impacts gene expression in the berries, through ontology categories that cover cell wall metabolism, primary and secondary metabolism, signaling, stress, and hormones, and that some of these effects strongly depend on the rootstock genotype. Indeed, under drought stress, berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate (JA), phenylpropanoid metabolism, and pathogenesis-related proteins. The data also suggest a link between JA and secondary metabolism in water-stressed berries. Overall, genes related to secondary metabolism and JA are more induced and/or less repressed by drought stress in the berries grafted on the drought-sensitive rootstock 125AA. These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

No MeSH data available.


Related in: MedlinePlus