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Water limitation and rootstock genotype interact to alter grape berry metabolism through transcriptome reprogramming.

Berdeja M, Nicolas P, Kappel C, Dai ZW, Hilbert G, Peccoux A, Lafontaine M, Ollat N, Gomès E, Delrot S - Hortic Res (2015)

Bottom Line: Pinot noir) from vines grafted on either 110R (drought-tolerant) or 125AA (drought-sensitive) rootstock were compared.Indeed, under drought stress, berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate (JA), phenylpropanoid metabolism, and pathogenesis-related proteins.These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

View Article: PubMed Central - PubMed

Affiliation: Ecophysiologie et Génomique Fonctionnelle de la Vigne, University Bordeaux, ISVV, UMR 1287 , Villenave d'Ornon F-33140, France.

ABSTRACT
Grapevine is a perennial crop often cultivated by grafting a scion cultivar on a suitable rootstock. Rootstocks influence scions, particularly with regard to water uptake and vigor. Therefore, one of the possibilities to adapt viticulture to the extended drought stress periods is to select rootstocks conferring increased tolerance to drought. However, the molecular mechanisms associated with the ability of rootstock/scion combination to influence grape berry metabolism under drought stress are still poorly understood. The transcriptomic changes induced by drought stress in grape berries (cv. Pinot noir) from vines grafted on either 110R (drought-tolerant) or 125AA (drought-sensitive) rootstock were compared. The experiments were conducted in the vineyard for two years and two grape berry developmental stages (50% and 100% veraison). The genome-wide microarray approach showed that water stress strongly impacts gene expression in the berries, through ontology categories that cover cell wall metabolism, primary and secondary metabolism, signaling, stress, and hormones, and that some of these effects strongly depend on the rootstock genotype. Indeed, under drought stress, berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate (JA), phenylpropanoid metabolism, and pathogenesis-related proteins. The data also suggest a link between JA and secondary metabolism in water-stressed berries. Overall, genes related to secondary metabolism and JA are more induced and/or less repressed by drought stress in the berries grafted on the drought-sensitive rootstock 125AA. These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

No MeSH data available.


Related in: MedlinePlus

Two-way Venn diagram of the genes differentially expressed at E-L 35 and E-L 36 for the log2(110R-S/110R-C) and log2(125AA-S/125AA-C) conditions in 2009 and 2010.
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fig1: Two-way Venn diagram of the genes differentially expressed at E-L 35 and E-L 36 for the log2(110R-S/110R-C) and log2(125AA-S/125AA-C) conditions in 2009 and 2010.

Mentions: Venn diagrams (Figure 1) shows that at E-L 35, more genes were affected in berries grafted in 110R than in berries grafted on 125AA, while the reverse was true at E-L 36. The proportion of upregulated genes shared for both years ranged between 10 and 30% of the total number of upregulated genes, except for E-L 36 125AA, where this figure drops down to less than 10%. Among the genes differentially expressed for both years, more genes were upregulated than downregulated by water stress, except for E-L 36 125AA. The proportion of downregulated genes shared for both years is 10–20% (Figure 1).


Water limitation and rootstock genotype interact to alter grape berry metabolism through transcriptome reprogramming.

Berdeja M, Nicolas P, Kappel C, Dai ZW, Hilbert G, Peccoux A, Lafontaine M, Ollat N, Gomès E, Delrot S - Hortic Res (2015)

Two-way Venn diagram of the genes differentially expressed at E-L 35 and E-L 36 for the log2(110R-S/110R-C) and log2(125AA-S/125AA-C) conditions in 2009 and 2010.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595978&req=5

fig1: Two-way Venn diagram of the genes differentially expressed at E-L 35 and E-L 36 for the log2(110R-S/110R-C) and log2(125AA-S/125AA-C) conditions in 2009 and 2010.
Mentions: Venn diagrams (Figure 1) shows that at E-L 35, more genes were affected in berries grafted in 110R than in berries grafted on 125AA, while the reverse was true at E-L 36. The proportion of upregulated genes shared for both years ranged between 10 and 30% of the total number of upregulated genes, except for E-L 36 125AA, where this figure drops down to less than 10%. Among the genes differentially expressed for both years, more genes were upregulated than downregulated by water stress, except for E-L 36 125AA. The proportion of downregulated genes shared for both years is 10–20% (Figure 1).

Bottom Line: Pinot noir) from vines grafted on either 110R (drought-tolerant) or 125AA (drought-sensitive) rootstock were compared.Indeed, under drought stress, berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate (JA), phenylpropanoid metabolism, and pathogenesis-related proteins.These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

View Article: PubMed Central - PubMed

Affiliation: Ecophysiologie et Génomique Fonctionnelle de la Vigne, University Bordeaux, ISVV, UMR 1287 , Villenave d'Ornon F-33140, France.

ABSTRACT
Grapevine is a perennial crop often cultivated by grafting a scion cultivar on a suitable rootstock. Rootstocks influence scions, particularly with regard to water uptake and vigor. Therefore, one of the possibilities to adapt viticulture to the extended drought stress periods is to select rootstocks conferring increased tolerance to drought. However, the molecular mechanisms associated with the ability of rootstock/scion combination to influence grape berry metabolism under drought stress are still poorly understood. The transcriptomic changes induced by drought stress in grape berries (cv. Pinot noir) from vines grafted on either 110R (drought-tolerant) or 125AA (drought-sensitive) rootstock were compared. The experiments were conducted in the vineyard for two years and two grape berry developmental stages (50% and 100% veraison). The genome-wide microarray approach showed that water stress strongly impacts gene expression in the berries, through ontology categories that cover cell wall metabolism, primary and secondary metabolism, signaling, stress, and hormones, and that some of these effects strongly depend on the rootstock genotype. Indeed, under drought stress, berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate (JA), phenylpropanoid metabolism, and pathogenesis-related proteins. The data also suggest a link between JA and secondary metabolism in water-stressed berries. Overall, genes related to secondary metabolism and JA are more induced and/or less repressed by drought stress in the berries grafted on the drought-sensitive rootstock 125AA. These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

No MeSH data available.


Related in: MedlinePlus