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Mapping QTL associated with Verticillium dahliae resistance in the cultivated strawberry (Fragaria × ananassa).

Antanaviciute L, Šurbanovski N, Harrison N, McLeary KJ, Simpson DW, Wilson F, Sargent DJ, Harrison RJ - Hortic Res (2015)

Bottom Line: Average wilt scores were significantly associated with multiple QTL, which were mostly significant across all years.A clear and statistically significant relationship was observed between resistant, tolerant and susceptible material and the total number of markers present in the different resistance classes.These markers are abundant in the cultivated strawberry germplasm indicating that, despite the large number of markers, clear genetic gain is possible through marker-assisted breeding.

View Article: PubMed Central - PubMed

Affiliation: East Malling Research, New Road, East Malling , Kent ME19 6BJ, UK.

ABSTRACT
A biparental cross of octoploid strawberry segregating for resistance to Verticillium dahliae, the causative agent of Verticillium wilt, was screened under field conditions for three seasons. Average wilt scores were significantly associated with multiple QTL, which were mostly significant across all years. Markers significantly associated with the traits were used to screen material with known wilt resistance and susceptibility phenotypes. A clear and statistically significant relationship was observed between resistant, tolerant and susceptible material and the total number of markers present in the different resistance classes. In field situations resistance QTL appear to behave in an additive manner. These markers are abundant in the cultivated strawberry germplasm indicating that, despite the large number of markers, clear genetic gain is possible through marker-assisted breeding.

No MeSH data available.


(a) A histogram depicting resistance to Verticillium dahliae in ‘Redgauntlet’ × ‘Hapil’ progeny in 2009. Overlaid in blue is a normal distribution for the mean and standard deviation of the untransformed phenotypic data. The red arrow indicates the mean phenotypic score of ‘Redgauntlet’, while the blue arrow indicates the mean phenotypic score of ‘Hapil’. (b) As a but data from 2010. (c) As a but data from 2011.
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fig3: (a) A histogram depicting resistance to Verticillium dahliae in ‘Redgauntlet’ × ‘Hapil’ progeny in 2009. Overlaid in blue is a normal distribution for the mean and standard deviation of the untransformed phenotypic data. The red arrow indicates the mean phenotypic score of ‘Redgauntlet’, while the blue arrow indicates the mean phenotypic score of ‘Hapil’. (b) As a but data from 2010. (c) As a but data from 2011.

Mentions: The ‘Redgauntlet’ × ‘Hapil’ mapping population was phenotyped in the same way as the long-term resistance experiment, in the field in a randomized block design, with a minimum of six replicates per year. In 2009, the population was phenotyped extensively, along with a standard variety (‘Elsanta’) in order to determine the optimal time for phenotypic evaluation. Figure 2 illustrates the development of above-ground disease symptoms over the season. Comparison of the tails of the mapping population with the two parents and the additional cultivars from the long-term experiment revealed that development of disease symptoms is approximately linear across the season. From the mapping population, observation of the distributions of resistance revealed that the distribution of disease symptoms was most symmetrical and closest to a normal distribution in the two weeks between the end of July and the start of August in all years (Figure 3).


Mapping QTL associated with Verticillium dahliae resistance in the cultivated strawberry (Fragaria × ananassa).

Antanaviciute L, Šurbanovski N, Harrison N, McLeary KJ, Simpson DW, Wilson F, Sargent DJ, Harrison RJ - Hortic Res (2015)

(a) A histogram depicting resistance to Verticillium dahliae in ‘Redgauntlet’ × ‘Hapil’ progeny in 2009. Overlaid in blue is a normal distribution for the mean and standard deviation of the untransformed phenotypic data. The red arrow indicates the mean phenotypic score of ‘Redgauntlet’, while the blue arrow indicates the mean phenotypic score of ‘Hapil’. (b) As a but data from 2010. (c) As a but data from 2011.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4595976&req=5

fig3: (a) A histogram depicting resistance to Verticillium dahliae in ‘Redgauntlet’ × ‘Hapil’ progeny in 2009. Overlaid in blue is a normal distribution for the mean and standard deviation of the untransformed phenotypic data. The red arrow indicates the mean phenotypic score of ‘Redgauntlet’, while the blue arrow indicates the mean phenotypic score of ‘Hapil’. (b) As a but data from 2010. (c) As a but data from 2011.
Mentions: The ‘Redgauntlet’ × ‘Hapil’ mapping population was phenotyped in the same way as the long-term resistance experiment, in the field in a randomized block design, with a minimum of six replicates per year. In 2009, the population was phenotyped extensively, along with a standard variety (‘Elsanta’) in order to determine the optimal time for phenotypic evaluation. Figure 2 illustrates the development of above-ground disease symptoms over the season. Comparison of the tails of the mapping population with the two parents and the additional cultivars from the long-term experiment revealed that development of disease symptoms is approximately linear across the season. From the mapping population, observation of the distributions of resistance revealed that the distribution of disease symptoms was most symmetrical and closest to a normal distribution in the two weeks between the end of July and the start of August in all years (Figure 3).

Bottom Line: Average wilt scores were significantly associated with multiple QTL, which were mostly significant across all years.A clear and statistically significant relationship was observed between resistant, tolerant and susceptible material and the total number of markers present in the different resistance classes.These markers are abundant in the cultivated strawberry germplasm indicating that, despite the large number of markers, clear genetic gain is possible through marker-assisted breeding.

View Article: PubMed Central - PubMed

Affiliation: East Malling Research, New Road, East Malling , Kent ME19 6BJ, UK.

ABSTRACT
A biparental cross of octoploid strawberry segregating for resistance to Verticillium dahliae, the causative agent of Verticillium wilt, was screened under field conditions for three seasons. Average wilt scores were significantly associated with multiple QTL, which were mostly significant across all years. Markers significantly associated with the traits were used to screen material with known wilt resistance and susceptibility phenotypes. A clear and statistically significant relationship was observed between resistant, tolerant and susceptible material and the total number of markers present in the different resistance classes. In field situations resistance QTL appear to behave in an additive manner. These markers are abundant in the cultivated strawberry germplasm indicating that, despite the large number of markers, clear genetic gain is possible through marker-assisted breeding.

No MeSH data available.