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Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model.

Mittal R, Sharma A, Arora S - J Pharm (Cairo) (2012)

Bottom Line: The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

View Article: PubMed Central - PubMed

Affiliation: Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.

ABSTRACT
The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

No MeSH data available.


Related in: MedlinePlus

SEM photomicrograph (a) control, (b) liposomal formulation, (c) plain gel, and (d) UF treated rat skin.
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Related In: Results  -  Collection


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fig7: SEM photomicrograph (a) control, (b) liposomal formulation, (c) plain gel, and (d) UF treated rat skin.

Mentions: SEM studies using rat skin was conducted in order to explain the effect of oleic acid formulation on the surface morphology of skin. Figure 7 shows the SEM photomicrograph of rat skin treated with PBS (pH 7.4) acting as control, plain ufasomal gel, liposomal formulation, and plain gel. In rat skin incubated with PBS there was absence of intracellular vesicular structures in stratum corneum. However ufasomes were visualized on the surface of stratum corneum. The vesicular suspension formed networks and stacks of lipid bilayers at the interface of the stratum corneum (Figure 7). Intracellular vesicular structures were observed in superficial layers of the stratum corneum and their appearance might be explained by desquamating corneocytes with a leaky membrane, through which oleic acid vesicles penetrate. These vesicular suspensions are more flexible and can easily pass through skin pores. Skin treated with UF complex have rough surface with pore formation. Rough surface is probably due to the lipid perturbation effect of oleic acid.


Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model.

Mittal R, Sharma A, Arora S - J Pharm (Cairo) (2012)

SEM photomicrograph (a) control, (b) liposomal formulation, (c) plain gel, and (d) UF treated rat skin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4595971&req=5

fig7: SEM photomicrograph (a) control, (b) liposomal formulation, (c) plain gel, and (d) UF treated rat skin.
Mentions: SEM studies using rat skin was conducted in order to explain the effect of oleic acid formulation on the surface morphology of skin. Figure 7 shows the SEM photomicrograph of rat skin treated with PBS (pH 7.4) acting as control, plain ufasomal gel, liposomal formulation, and plain gel. In rat skin incubated with PBS there was absence of intracellular vesicular structures in stratum corneum. However ufasomes were visualized on the surface of stratum corneum. The vesicular suspension formed networks and stacks of lipid bilayers at the interface of the stratum corneum (Figure 7). Intracellular vesicular structures were observed in superficial layers of the stratum corneum and their appearance might be explained by desquamating corneocytes with a leaky membrane, through which oleic acid vesicles penetrate. These vesicular suspensions are more flexible and can easily pass through skin pores. Skin treated with UF complex have rough surface with pore formation. Rough surface is probably due to the lipid perturbation effect of oleic acid.

Bottom Line: The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

View Article: PubMed Central - PubMed

Affiliation: Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.

ABSTRACT
The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

No MeSH data available.


Related in: MedlinePlus