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Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model.

Mittal R, Sharma A, Arora S - J Pharm (Cairo) (2012)

Bottom Line: The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

View Article: PubMed Central - PubMed

Affiliation: Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.

ABSTRACT
The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

No MeSH data available.


Related in: MedlinePlus

% Cumulative drug permeation through rat skin of plain drug gel and optimized formulation.
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fig6: % Cumulative drug permeation through rat skin of plain drug gel and optimized formulation.

Mentions: Determination of Amount of Drug Deposited into the Skin. In this method the in vitro drug permeation study was performed in two stages using the same locally fabricated diffusion cell. In the first stage PBS (pH 7.4) was used as the receptor medium and method as described above for skin permeation was carried out. UF-2 (2 mL) was applied to epidermal surface of rat skin. Samples were withdrawn through the sampling port of the diffusion cell at predetermined intervals over 10 hr and analyzed. The receptor phase was immediately replenished with equal volume of fresh buffer. At the end of 10 hr the donor compartment was washed five times with warm receptor fluid. The second stage used 50% v/v ethanol as the receptor solution for a further period of 12 hr and performed without any donor phase. During this stage an ethanolic receptor will diffuse into the skin disrupting the vesicular structure of any UF-2 that may have penetrated and deposited in the tissue and thus releasing both UF bound and free dexamethasone for collection by receptor fluid (Figure 6). Use of 50% ethanol as receptor fluid can slightly reduce the barrier nature of stratum corneum; hence, the second stage was performed after removal of the donor to avoid any excess permeation due to penetration enhancing activity of ethanol [24].


Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model.

Mittal R, Sharma A, Arora S - J Pharm (Cairo) (2012)

% Cumulative drug permeation through rat skin of plain drug gel and optimized formulation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4595971&req=5

fig6: % Cumulative drug permeation through rat skin of plain drug gel and optimized formulation.
Mentions: Determination of Amount of Drug Deposited into the Skin. In this method the in vitro drug permeation study was performed in two stages using the same locally fabricated diffusion cell. In the first stage PBS (pH 7.4) was used as the receptor medium and method as described above for skin permeation was carried out. UF-2 (2 mL) was applied to epidermal surface of rat skin. Samples were withdrawn through the sampling port of the diffusion cell at predetermined intervals over 10 hr and analyzed. The receptor phase was immediately replenished with equal volume of fresh buffer. At the end of 10 hr the donor compartment was washed five times with warm receptor fluid. The second stage used 50% v/v ethanol as the receptor solution for a further period of 12 hr and performed without any donor phase. During this stage an ethanolic receptor will diffuse into the skin disrupting the vesicular structure of any UF-2 that may have penetrated and deposited in the tissue and thus releasing both UF bound and free dexamethasone for collection by receptor fluid (Figure 6). Use of 50% ethanol as receptor fluid can slightly reduce the barrier nature of stratum corneum; hence, the second stage was performed after removal of the donor to avoid any excess permeation due to penetration enhancing activity of ethanol [24].

Bottom Line: The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

View Article: PubMed Central - PubMed

Affiliation: Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.

ABSTRACT
The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

No MeSH data available.


Related in: MedlinePlus