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Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model.

Mittal R, Sharma A, Arora S - J Pharm (Cairo) (2012)

Bottom Line: The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

View Article: PubMed Central - PubMed

Affiliation: Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.

ABSTRACT
The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

No MeSH data available.


Related in: MedlinePlus

pH-dependent release behavior of drug from oleic acid vesicles dispersion.
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Related In: Results  -  Collection


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fig4: pH-dependent release behavior of drug from oleic acid vesicles dispersion.

Mentions: The effect of pH on the stability and on the drug release behavior was monitored by incubating optimized vesicular dispersion with buffers of pH 8.5, 7.4, 6.5, and 5.5. At predetermined time intervals the samples were withdrawn and centrifuged at 14,000 rpm for 30 min [22]. The supernatant was analyzed for released free drug. The amount of drug leached was then calculated by the following formula:(2)%Drug  diffused=Amount  of  free  drugTotal  drug×100.Simultaneously, the incubated vesicles were observed for any change in morphology and size using an optical microscope. The studies were performed in triplicate (Figures 4 and 5).


Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model.

Mittal R, Sharma A, Arora S - J Pharm (Cairo) (2012)

pH-dependent release behavior of drug from oleic acid vesicles dispersion.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4595971&req=5

fig4: pH-dependent release behavior of drug from oleic acid vesicles dispersion.
Mentions: The effect of pH on the stability and on the drug release behavior was monitored by incubating optimized vesicular dispersion with buffers of pH 8.5, 7.4, 6.5, and 5.5. At predetermined time intervals the samples were withdrawn and centrifuged at 14,000 rpm for 30 min [22]. The supernatant was analyzed for released free drug. The amount of drug leached was then calculated by the following formula:(2)%Drug  diffused=Amount  of  free  drugTotal  drug×100.Simultaneously, the incubated vesicles were observed for any change in morphology and size using an optical microscope. The studies were performed in triplicate (Figures 4 and 5).

Bottom Line: The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

View Article: PubMed Central - PubMed

Affiliation: Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.

ABSTRACT
The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

No MeSH data available.


Related in: MedlinePlus