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Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model.

Mittal R, Sharma A, Arora S - J Pharm (Cairo) (2012)

Bottom Line: The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

View Article: PubMed Central - PubMed

Affiliation: Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.

ABSTRACT
The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

No MeSH data available.


Related in: MedlinePlus

Dynamic light scattering (DLS) analysis of UF-2 formulation.
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Related In: Results  -  Collection


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fig2: Dynamic light scattering (DLS) analysis of UF-2 formulation.

Mentions: The size and size distribution of vesicles with different composition shown in Table 1 were determined by optical microscopy using stage eyepiece micrometer calibrated using micrometer scale. After sonication the vesicle size was determined by dynamic light scattering method (DLS), using a computerized inspection system (Malvern Zetamaster, ZEM 5002, Malvern, UK) and Figure 2 shows vesicle size distribution of optimized formulation. For vesicles size measurement the vesicular preparation was mixed with appropriate medium (for ufasomal formulation with 7% v/v ethanol) and filtered through 0.2 μm polycarbonate membrane to minimize interference from particular matter. The measurements were conducted in triplicate in a multimodal mode of 200 and each at a medium stable count rate.


Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model.

Mittal R, Sharma A, Arora S - J Pharm (Cairo) (2012)

Dynamic light scattering (DLS) analysis of UF-2 formulation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4595971&req=5

fig2: Dynamic light scattering (DLS) analysis of UF-2 formulation.
Mentions: The size and size distribution of vesicles with different composition shown in Table 1 were determined by optical microscopy using stage eyepiece micrometer calibrated using micrometer scale. After sonication the vesicle size was determined by dynamic light scattering method (DLS), using a computerized inspection system (Malvern Zetamaster, ZEM 5002, Malvern, UK) and Figure 2 shows vesicle size distribution of optimized formulation. For vesicles size measurement the vesicular preparation was mixed with appropriate medium (for ufasomal formulation with 7% v/v ethanol) and filtered through 0.2 μm polycarbonate membrane to minimize interference from particular matter. The measurements were conducted in triplicate in a multimodal mode of 200 and each at a medium stable count rate.

Bottom Line: The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

View Article: PubMed Central - PubMed

Affiliation: Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India.

ABSTRACT
The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

No MeSH data available.


Related in: MedlinePlus