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Use of the Charge Transfer Reactions for the Spectrophotometric Determination of Risperidone in Pure and in Dosage Forms.

Deepakumari HN, Revanasiddappa HD - J Pharm (Cairo) (2012)

Bottom Line: Beer's law was obeyed in the concentration range of 0-25 and 0-50 μg/mL with molar absorptivity of 1.29 × 10(4) and 0.48 × 10(4) L/moL/cm for RSP in methods A and B, respectively.The effects of variables such as reagents, time, and stability of the charge transfer complexes were investigated to optimize the procedures.Results indicate that the methods are accurate, precise, and reproducible (relative standard deviation <2 %).

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Mysore, Manasagangothri, Mysore 570006, India.

ABSTRACT
The aim of study was to develop and validate two simple, sensitive, and extraction-free spectrophotometric methods for the estimation of risperidone in both pure and pharmaceutical preparations. They are based on the charge transfer complexation reactions between risperidone (RSP) as n-electron donor and p-chloranilic acid (p-CA) in method A and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in method B as π-acceptors. In method A, RSP reacts with p-CA in methanol to produce a bright pink-colored chromogen measured at 530 nm whereas, in method B, RSP reacts with DDQ in dichloromethane to form orange-colored complex with a maximum absorption at 460 nm. Beer's law was obeyed in the concentration range of 0-25 and 0-50 μg/mL with molar absorptivity of 1.29 × 10(4) and 0.48 × 10(4) L/moL/cm for RSP in methods A and B, respectively. The effects of variables such as reagents, time, and stability of the charge transfer complexes were investigated to optimize the procedures. The proposed methods have been successfully applied to the determination of RSP in pharmaceutical formulations. Results indicate that the methods are accurate, precise, and reproducible (relative standard deviation <2 %).

No MeSH data available.


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Job's plot for stoichiometric ratio for (a) (RSP) and (p-CA) = (1.22 × 10−4 M) and (b) (RSP) and (DDQ) = (2.44 × 10−4 M).
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fig2: Job's plot for stoichiometric ratio for (a) (RSP) and (p-CA) = (1.22 × 10−4 M) and (b) (RSP) and (DDQ) = (2.44 × 10−4 M).

Mentions: Job's method of continuous variation [15] was employed to establish the stoichiometry of the colored products. The solutions equivalent to 1.22 × 10−4 and 2.44 × 10−4 M RSP were prepared. Further, 1.22 × 10−4 M p-CA and 2.44 × 10−4 M DDQ solutions were prepared in acetone and acetonitrile, respectively. A series of solutions were mixed in complimentary proportions; in method A, the volume was completed up to the mark using methanol, and with dichloromethane in method B. The absorbances of the resulting solutions were measured at their respective wavelengths (λmax) against the reagent blank under the similar conditions. Job's method of continuous variations graph for the reaction between RSP and p-CA or DDQ (Figure 2) shows that the interaction occurs on an equimolar basis via the formation of charge-transfer complexes in the ratio 1 : 1 (RSP : p-CA or DDQ).


Use of the Charge Transfer Reactions for the Spectrophotometric Determination of Risperidone in Pure and in Dosage Forms.

Deepakumari HN, Revanasiddappa HD - J Pharm (Cairo) (2012)

Job's plot for stoichiometric ratio for (a) (RSP) and (p-CA) = (1.22 × 10−4 M) and (b) (RSP) and (DDQ) = (2.44 × 10−4 M).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4595964&req=5

fig2: Job's plot for stoichiometric ratio for (a) (RSP) and (p-CA) = (1.22 × 10−4 M) and (b) (RSP) and (DDQ) = (2.44 × 10−4 M).
Mentions: Job's method of continuous variation [15] was employed to establish the stoichiometry of the colored products. The solutions equivalent to 1.22 × 10−4 and 2.44 × 10−4 M RSP were prepared. Further, 1.22 × 10−4 M p-CA and 2.44 × 10−4 M DDQ solutions were prepared in acetone and acetonitrile, respectively. A series of solutions were mixed in complimentary proportions; in method A, the volume was completed up to the mark using methanol, and with dichloromethane in method B. The absorbances of the resulting solutions were measured at their respective wavelengths (λmax) against the reagent blank under the similar conditions. Job's method of continuous variations graph for the reaction between RSP and p-CA or DDQ (Figure 2) shows that the interaction occurs on an equimolar basis via the formation of charge-transfer complexes in the ratio 1 : 1 (RSP : p-CA or DDQ).

Bottom Line: Beer's law was obeyed in the concentration range of 0-25 and 0-50 μg/mL with molar absorptivity of 1.29 × 10(4) and 0.48 × 10(4) L/moL/cm for RSP in methods A and B, respectively.The effects of variables such as reagents, time, and stability of the charge transfer complexes were investigated to optimize the procedures.Results indicate that the methods are accurate, precise, and reproducible (relative standard deviation <2 %).

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Mysore, Manasagangothri, Mysore 570006, India.

ABSTRACT
The aim of study was to develop and validate two simple, sensitive, and extraction-free spectrophotometric methods for the estimation of risperidone in both pure and pharmaceutical preparations. They are based on the charge transfer complexation reactions between risperidone (RSP) as n-electron donor and p-chloranilic acid (p-CA) in method A and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in method B as π-acceptors. In method A, RSP reacts with p-CA in methanol to produce a bright pink-colored chromogen measured at 530 nm whereas, in method B, RSP reacts with DDQ in dichloromethane to form orange-colored complex with a maximum absorption at 460 nm. Beer's law was obeyed in the concentration range of 0-25 and 0-50 μg/mL with molar absorptivity of 1.29 × 10(4) and 0.48 × 10(4) L/moL/cm for RSP in methods A and B, respectively. The effects of variables such as reagents, time, and stability of the charge transfer complexes were investigated to optimize the procedures. The proposed methods have been successfully applied to the determination of RSP in pharmaceutical formulations. Results indicate that the methods are accurate, precise, and reproducible (relative standard deviation <2 %).

No MeSH data available.


Related in: MedlinePlus