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Analysis of Hydroxy Fatty Acids from the Pollen of Brassica campestris L. var. oleifera DC. by UPLC-MS/MS.

Yang NY, Yang YF, Li K - J Pharm (Cairo) (2012)

Bottom Line: Ultraperformance liquid chromatography coupled with negative electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) was used to determine 7 hydroxy fatty acids in the pollen of Brassica campestris L. var. oleifera DC.All the investigated hydroxy fatty acids showed strong deprotonated molecular ions [M-H](-), which underwent two major fragment pathways of the allyl scission and the β-fission of the alcoholic hydroxyl group.Compounds 3, 5, and 7 are reported for the first time.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China.

ABSTRACT
Ultraperformance liquid chromatography coupled with negative electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) was used to determine 7 hydroxy fatty acids in the pollen of Brassica campestris L. var. oleifera DC. All the investigated hydroxy fatty acids showed strong deprotonated molecular ions [M-H](-), which underwent two major fragment pathways of the allyl scission and the β-fission of the alcoholic hydroxyl group. By comparison of their molecular ions and abundant fragment ions with those of reference compounds, they were tentatively assigned as 15,16-dihydroxy-9Z,12Z-octadecadienoic acid (1), 10,11,12-trihydroxy-(7Z,14Z)-heptadecadienoic acid (2), 7,15,16-trihydroxy-9Z,12Z-octadecadienoic acid (3), 15,16-dihydroxy-9Z,12Z-octadecadienoic acid (4), 15-hydroxy-6Z,9Z,12Z-octadecatrienoic acid (5), 15-hydroxy-9Z,12Z- octadecadienoic acid (6), and 15-hydroxy-12Z-octadecaenoic acid (7), respectively. Compounds 3, 5, and 7 are reported for the first time.

No MeSH data available.


Total ion chromatogram of supercritical fluid extract of pollen of Brassica campestris L. var. oleifera DC.
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fig2: Total ion chromatogram of supercritical fluid extract of pollen of Brassica campestris L. var. oleifera DC.

Mentions: UPLC-ESI-MS was used to analyze the chemical constituents of the supercritical fluid extract of pollen of B. campestris L. var. oleifera DC. (Figure 2), and the fragmentation behaviour of 7 hydroxy fatty acids was analyzed by UPLC-ESI-MS/MS. In the total ion chromatograms (Figure 2), the peaks at 9.21 min and 9.97 min showed a molecular ion [M–H]− at m/z 311 and 313, respectively (Figure 3), which were identical to compounds 1-2, respectively and confirmed by the same characteristic data of UPLC analysis. In the total ion chromatograms, peak at 8.15 min showed a molecular ion [M–H]− at m/z 327, the peak at 8.60 min showed a molecular ion [M–H]− at m/z 309, the peak at 11.10 min showed a molecular ion [M–H]− at m/z 293, the peak at 11.80 min showed a molecular ion [M–H]− at m/z 295, and the peak at 12.71 min showed a molecular ion [M–H]− at m/z 297, which were identified by UHPLC-MS/MS analysis. From the product ion spectra (Figure 3) of compound 3 at 8.15 min, compound 4 at 8.60 min, compound 5 at 11.10 min, compound 6 at 11.80 min, and compound 7 at 12.71 min, it was found that they showed almost the same fragment pattern with compounds 1-2.


Analysis of Hydroxy Fatty Acids from the Pollen of Brassica campestris L. var. oleifera DC. by UPLC-MS/MS.

Yang NY, Yang YF, Li K - J Pharm (Cairo) (2012)

Total ion chromatogram of supercritical fluid extract of pollen of Brassica campestris L. var. oleifera DC.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4595942&req=5

fig2: Total ion chromatogram of supercritical fluid extract of pollen of Brassica campestris L. var. oleifera DC.
Mentions: UPLC-ESI-MS was used to analyze the chemical constituents of the supercritical fluid extract of pollen of B. campestris L. var. oleifera DC. (Figure 2), and the fragmentation behaviour of 7 hydroxy fatty acids was analyzed by UPLC-ESI-MS/MS. In the total ion chromatograms (Figure 2), the peaks at 9.21 min and 9.97 min showed a molecular ion [M–H]− at m/z 311 and 313, respectively (Figure 3), which were identical to compounds 1-2, respectively and confirmed by the same characteristic data of UPLC analysis. In the total ion chromatograms, peak at 8.15 min showed a molecular ion [M–H]− at m/z 327, the peak at 8.60 min showed a molecular ion [M–H]− at m/z 309, the peak at 11.10 min showed a molecular ion [M–H]− at m/z 293, the peak at 11.80 min showed a molecular ion [M–H]− at m/z 295, and the peak at 12.71 min showed a molecular ion [M–H]− at m/z 297, which were identified by UHPLC-MS/MS analysis. From the product ion spectra (Figure 3) of compound 3 at 8.15 min, compound 4 at 8.60 min, compound 5 at 11.10 min, compound 6 at 11.80 min, and compound 7 at 12.71 min, it was found that they showed almost the same fragment pattern with compounds 1-2.

Bottom Line: Ultraperformance liquid chromatography coupled with negative electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) was used to determine 7 hydroxy fatty acids in the pollen of Brassica campestris L. var. oleifera DC.All the investigated hydroxy fatty acids showed strong deprotonated molecular ions [M-H](-), which underwent two major fragment pathways of the allyl scission and the β-fission of the alcoholic hydroxyl group.Compounds 3, 5, and 7 are reported for the first time.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China.

ABSTRACT
Ultraperformance liquid chromatography coupled with negative electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) was used to determine 7 hydroxy fatty acids in the pollen of Brassica campestris L. var. oleifera DC. All the investigated hydroxy fatty acids showed strong deprotonated molecular ions [M-H](-), which underwent two major fragment pathways of the allyl scission and the β-fission of the alcoholic hydroxyl group. By comparison of their molecular ions and abundant fragment ions with those of reference compounds, they were tentatively assigned as 15,16-dihydroxy-9Z,12Z-octadecadienoic acid (1), 10,11,12-trihydroxy-(7Z,14Z)-heptadecadienoic acid (2), 7,15,16-trihydroxy-9Z,12Z-octadecadienoic acid (3), 15,16-dihydroxy-9Z,12Z-octadecadienoic acid (4), 15-hydroxy-6Z,9Z,12Z-octadecatrienoic acid (5), 15-hydroxy-9Z,12Z- octadecadienoic acid (6), and 15-hydroxy-12Z-octadecaenoic acid (7), respectively. Compounds 3, 5, and 7 are reported for the first time.

No MeSH data available.